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Self-Assembling Protein Surfaces for In Situ Capture of Cell-Free-Synthesized Proteins

We present a new method for the surface capture of proteins in cell-free protein synthesis (CFPS). We demonstrate the spontaneous self-assembly of the protein BslA into functionalizable surfaces on the surface of a CFPS reaction chamber. We show that proteins can be covalently captured by such surfa...

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Detalles Bibliográficos
Autores principales:	Thornton, Ella Lucille, Paterson, Sarah Maria, Gidden, Zoe, Horrocks, Mathew H., Laohakunakorn, Nadanai, Regan, Lynne
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2022
Materias:	Bioengineering and Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300835/ https://www.ncbi.nlm.nih.gov/pubmed/35875503 http://dx.doi.org/10.3389/fbioe.2022.915035

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