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Production of a functionally active recombinant SARS-CoV-2 (COVID-19) 3C-like protease and a soluble inactive 3C-like protease-RBD chimeric in a prokaryotic expression system

During the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) intracellular life-cycle, two large polyproteins, pp1a and pp1ab, are produced. Processing of these by viral cysteine proteases, the papain-like protease (PLpro) and the chymotrypsin-like 3C-like protease (3CL-pro) release non-s...

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Detalles Bibliográficos
Autores principales:	De Marco Verissimo, Carolina, López Corrales, Jesús, Dorey, Amber L., Cwiklinski, Krystyna, Lalor, Richard, Calvani, Nichola Eliza Davies, Jewhurst, Heather L., Flaus, Andrew, Doyle, Sean, Dalton, John P.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cambridge University Press 2022
Materias:	Original Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300977/ https://www.ncbi.nlm.nih.gov/pubmed/35723031 http://dx.doi.org/10.1017/S0950268822001078

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