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High Genetic Diversity of Porcine Sapovirus From Diarrheic Piglets in Yunnan Province, China

As one of the most important enteric viruses, sapovirus (SaV) can infect humans and a variety of animals. Until now, 19 SaV genogroups have been identified, among which 4 from human (GI, GII, GIV, and GV) and 8 from swine (GIII, GV–GXI). Porcine sapovirus (PoSaV) GIII has been prevalent in China; ho...

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Autores principales: Liu, Xiao, Song, Chunlian, Liu, Yinghua, Qu, Kaixing, Bi, Junyu, Bi, Junlong, Wang, Yunhua, Yang, Ying, Sun, Junhua, Guo, Zhigang, Li, Ganwu, Liu, Jianping, Yin, Gefen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300989/
https://www.ncbi.nlm.nih.gov/pubmed/35873674
http://dx.doi.org/10.3389/fvets.2022.854905
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author Liu, Xiao
Song, Chunlian
Liu, Yinghua
Qu, Kaixing
Bi, Junyu
Bi, Junlong
Wang, Yunhua
Yang, Ying
Sun, Junhua
Guo, Zhigang
Li, Ganwu
Liu, Jianping
Yin, Gefen
author_facet Liu, Xiao
Song, Chunlian
Liu, Yinghua
Qu, Kaixing
Bi, Junyu
Bi, Junlong
Wang, Yunhua
Yang, Ying
Sun, Junhua
Guo, Zhigang
Li, Ganwu
Liu, Jianping
Yin, Gefen
author_sort Liu, Xiao
collection PubMed
description As one of the most important enteric viruses, sapovirus (SaV) can infect humans and a variety of animals. Until now, 19 SaV genogroups have been identified, among which 4 from human (GI, GII, GIV, and GV) and 8 from swine (GIII, GV–GXI). Porcine sapovirus (PoSaV) GIII has been prevalent in China; however, the status of PoSaV infection in Yunnan province remains unknown. In this study, 202 fecal samples were collected from piglets associated with outbreaks of acute diarrhea in Yunnan between January and May 2020. PoSaV detection revealed that the total PoSaV infection rate in Yunnan was 35.2%, with 21 PoSaV strains determined and phylogenetically analyzed. The phylogenetic tree analyses demonstrated that twenty PoSaV strains belonged to GIII and fell into five genotypes, whereas one PoSaV strain (YNQB) belonged to GV. Sequence alignments revealed deletions in VP2 region in 10 of the 20 GIII strains, as well as deletions and insertions in VP1 region of the GV strain (YNQB). Furthermore, genomic recombination analyses showed that two GIII strains (YNAN and YNJD) were recombinants, closely related to reference sequences MK965898 and LC215880, MK965898 and FJ387164, respectively. In summary, PoSaV-GIII strains were identified in Yunnan in 2020, and for the first time, a PoSaV-GV strain was identified from China, whereas the comprehensive analyses illustrated high genetic diversity of Yunnan PoSaV strains. This study may shed new light on the current PoSaV infections in Yunnan and pave the way toward further control of the PoSaV infections in China.
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spelling pubmed-93009892022-07-22 High Genetic Diversity of Porcine Sapovirus From Diarrheic Piglets in Yunnan Province, China Liu, Xiao Song, Chunlian Liu, Yinghua Qu, Kaixing Bi, Junyu Bi, Junlong Wang, Yunhua Yang, Ying Sun, Junhua Guo, Zhigang Li, Ganwu Liu, Jianping Yin, Gefen Front Vet Sci Veterinary Science As one of the most important enteric viruses, sapovirus (SaV) can infect humans and a variety of animals. Until now, 19 SaV genogroups have been identified, among which 4 from human (GI, GII, GIV, and GV) and 8 from swine (GIII, GV–GXI). Porcine sapovirus (PoSaV) GIII has been prevalent in China; however, the status of PoSaV infection in Yunnan province remains unknown. In this study, 202 fecal samples were collected from piglets associated with outbreaks of acute diarrhea in Yunnan between January and May 2020. PoSaV detection revealed that the total PoSaV infection rate in Yunnan was 35.2%, with 21 PoSaV strains determined and phylogenetically analyzed. The phylogenetic tree analyses demonstrated that twenty PoSaV strains belonged to GIII and fell into five genotypes, whereas one PoSaV strain (YNQB) belonged to GV. Sequence alignments revealed deletions in VP2 region in 10 of the 20 GIII strains, as well as deletions and insertions in VP1 region of the GV strain (YNQB). Furthermore, genomic recombination analyses showed that two GIII strains (YNAN and YNJD) were recombinants, closely related to reference sequences MK965898 and LC215880, MK965898 and FJ387164, respectively. In summary, PoSaV-GIII strains were identified in Yunnan in 2020, and for the first time, a PoSaV-GV strain was identified from China, whereas the comprehensive analyses illustrated high genetic diversity of Yunnan PoSaV strains. This study may shed new light on the current PoSaV infections in Yunnan and pave the way toward further control of the PoSaV infections in China. Frontiers Media S.A. 2022-07-07 /pmc/articles/PMC9300989/ /pubmed/35873674 http://dx.doi.org/10.3389/fvets.2022.854905 Text en Copyright © 2022 Liu, Song, Liu, Qu, Bi, Bi, Wang, Yang, Sun, Guo, Li, Liu and Yin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Liu, Xiao
Song, Chunlian
Liu, Yinghua
Qu, Kaixing
Bi, Junyu
Bi, Junlong
Wang, Yunhua
Yang, Ying
Sun, Junhua
Guo, Zhigang
Li, Ganwu
Liu, Jianping
Yin, Gefen
High Genetic Diversity of Porcine Sapovirus From Diarrheic Piglets in Yunnan Province, China
title High Genetic Diversity of Porcine Sapovirus From Diarrheic Piglets in Yunnan Province, China
title_full High Genetic Diversity of Porcine Sapovirus From Diarrheic Piglets in Yunnan Province, China
title_fullStr High Genetic Diversity of Porcine Sapovirus From Diarrheic Piglets in Yunnan Province, China
title_full_unstemmed High Genetic Diversity of Porcine Sapovirus From Diarrheic Piglets in Yunnan Province, China
title_short High Genetic Diversity of Porcine Sapovirus From Diarrheic Piglets in Yunnan Province, China
title_sort high genetic diversity of porcine sapovirus from diarrheic piglets in yunnan province, china
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300989/
https://www.ncbi.nlm.nih.gov/pubmed/35873674
http://dx.doi.org/10.3389/fvets.2022.854905
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