Cargando…
High Genetic Diversity of Porcine Sapovirus From Diarrheic Piglets in Yunnan Province, China
As one of the most important enteric viruses, sapovirus (SaV) can infect humans and a variety of animals. Until now, 19 SaV genogroups have been identified, among which 4 from human (GI, GII, GIV, and GV) and 8 from swine (GIII, GV–GXI). Porcine sapovirus (PoSaV) GIII has been prevalent in China; ho...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300989/ https://www.ncbi.nlm.nih.gov/pubmed/35873674 http://dx.doi.org/10.3389/fvets.2022.854905 |
_version_ | 1784751333897666560 |
---|---|
author | Liu, Xiao Song, Chunlian Liu, Yinghua Qu, Kaixing Bi, Junyu Bi, Junlong Wang, Yunhua Yang, Ying Sun, Junhua Guo, Zhigang Li, Ganwu Liu, Jianping Yin, Gefen |
author_facet | Liu, Xiao Song, Chunlian Liu, Yinghua Qu, Kaixing Bi, Junyu Bi, Junlong Wang, Yunhua Yang, Ying Sun, Junhua Guo, Zhigang Li, Ganwu Liu, Jianping Yin, Gefen |
author_sort | Liu, Xiao |
collection | PubMed |
description | As one of the most important enteric viruses, sapovirus (SaV) can infect humans and a variety of animals. Until now, 19 SaV genogroups have been identified, among which 4 from human (GI, GII, GIV, and GV) and 8 from swine (GIII, GV–GXI). Porcine sapovirus (PoSaV) GIII has been prevalent in China; however, the status of PoSaV infection in Yunnan province remains unknown. In this study, 202 fecal samples were collected from piglets associated with outbreaks of acute diarrhea in Yunnan between January and May 2020. PoSaV detection revealed that the total PoSaV infection rate in Yunnan was 35.2%, with 21 PoSaV strains determined and phylogenetically analyzed. The phylogenetic tree analyses demonstrated that twenty PoSaV strains belonged to GIII and fell into five genotypes, whereas one PoSaV strain (YNQB) belonged to GV. Sequence alignments revealed deletions in VP2 region in 10 of the 20 GIII strains, as well as deletions and insertions in VP1 region of the GV strain (YNQB). Furthermore, genomic recombination analyses showed that two GIII strains (YNAN and YNJD) were recombinants, closely related to reference sequences MK965898 and LC215880, MK965898 and FJ387164, respectively. In summary, PoSaV-GIII strains were identified in Yunnan in 2020, and for the first time, a PoSaV-GV strain was identified from China, whereas the comprehensive analyses illustrated high genetic diversity of Yunnan PoSaV strains. This study may shed new light on the current PoSaV infections in Yunnan and pave the way toward further control of the PoSaV infections in China. |
format | Online Article Text |
id | pubmed-9300989 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93009892022-07-22 High Genetic Diversity of Porcine Sapovirus From Diarrheic Piglets in Yunnan Province, China Liu, Xiao Song, Chunlian Liu, Yinghua Qu, Kaixing Bi, Junyu Bi, Junlong Wang, Yunhua Yang, Ying Sun, Junhua Guo, Zhigang Li, Ganwu Liu, Jianping Yin, Gefen Front Vet Sci Veterinary Science As one of the most important enteric viruses, sapovirus (SaV) can infect humans and a variety of animals. Until now, 19 SaV genogroups have been identified, among which 4 from human (GI, GII, GIV, and GV) and 8 from swine (GIII, GV–GXI). Porcine sapovirus (PoSaV) GIII has been prevalent in China; however, the status of PoSaV infection in Yunnan province remains unknown. In this study, 202 fecal samples were collected from piglets associated with outbreaks of acute diarrhea in Yunnan between January and May 2020. PoSaV detection revealed that the total PoSaV infection rate in Yunnan was 35.2%, with 21 PoSaV strains determined and phylogenetically analyzed. The phylogenetic tree analyses demonstrated that twenty PoSaV strains belonged to GIII and fell into five genotypes, whereas one PoSaV strain (YNQB) belonged to GV. Sequence alignments revealed deletions in VP2 region in 10 of the 20 GIII strains, as well as deletions and insertions in VP1 region of the GV strain (YNQB). Furthermore, genomic recombination analyses showed that two GIII strains (YNAN and YNJD) were recombinants, closely related to reference sequences MK965898 and LC215880, MK965898 and FJ387164, respectively. In summary, PoSaV-GIII strains were identified in Yunnan in 2020, and for the first time, a PoSaV-GV strain was identified from China, whereas the comprehensive analyses illustrated high genetic diversity of Yunnan PoSaV strains. This study may shed new light on the current PoSaV infections in Yunnan and pave the way toward further control of the PoSaV infections in China. Frontiers Media S.A. 2022-07-07 /pmc/articles/PMC9300989/ /pubmed/35873674 http://dx.doi.org/10.3389/fvets.2022.854905 Text en Copyright © 2022 Liu, Song, Liu, Qu, Bi, Bi, Wang, Yang, Sun, Guo, Li, Liu and Yin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Liu, Xiao Song, Chunlian Liu, Yinghua Qu, Kaixing Bi, Junyu Bi, Junlong Wang, Yunhua Yang, Ying Sun, Junhua Guo, Zhigang Li, Ganwu Liu, Jianping Yin, Gefen High Genetic Diversity of Porcine Sapovirus From Diarrheic Piglets in Yunnan Province, China |
title | High Genetic Diversity of Porcine Sapovirus From Diarrheic Piglets in Yunnan Province, China |
title_full | High Genetic Diversity of Porcine Sapovirus From Diarrheic Piglets in Yunnan Province, China |
title_fullStr | High Genetic Diversity of Porcine Sapovirus From Diarrheic Piglets in Yunnan Province, China |
title_full_unstemmed | High Genetic Diversity of Porcine Sapovirus From Diarrheic Piglets in Yunnan Province, China |
title_short | High Genetic Diversity of Porcine Sapovirus From Diarrheic Piglets in Yunnan Province, China |
title_sort | high genetic diversity of porcine sapovirus from diarrheic piglets in yunnan province, china |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300989/ https://www.ncbi.nlm.nih.gov/pubmed/35873674 http://dx.doi.org/10.3389/fvets.2022.854905 |
work_keys_str_mv | AT liuxiao highgeneticdiversityofporcinesapovirusfromdiarrheicpigletsinyunnanprovincechina AT songchunlian highgeneticdiversityofporcinesapovirusfromdiarrheicpigletsinyunnanprovincechina AT liuyinghua highgeneticdiversityofporcinesapovirusfromdiarrheicpigletsinyunnanprovincechina AT qukaixing highgeneticdiversityofporcinesapovirusfromdiarrheicpigletsinyunnanprovincechina AT bijunyu highgeneticdiversityofporcinesapovirusfromdiarrheicpigletsinyunnanprovincechina AT bijunlong highgeneticdiversityofporcinesapovirusfromdiarrheicpigletsinyunnanprovincechina AT wangyunhua highgeneticdiversityofporcinesapovirusfromdiarrheicpigletsinyunnanprovincechina AT yangying highgeneticdiversityofporcinesapovirusfromdiarrheicpigletsinyunnanprovincechina AT sunjunhua highgeneticdiversityofporcinesapovirusfromdiarrheicpigletsinyunnanprovincechina AT guozhigang highgeneticdiversityofporcinesapovirusfromdiarrheicpigletsinyunnanprovincechina AT liganwu highgeneticdiversityofporcinesapovirusfromdiarrheicpigletsinyunnanprovincechina AT liujianping highgeneticdiversityofporcinesapovirusfromdiarrheicpigletsinyunnanprovincechina AT yingefen highgeneticdiversityofporcinesapovirusfromdiarrheicpigletsinyunnanprovincechina |