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Preparation and Structure Characterization of High-Value Laminaria digitata Oligosaccharides

Algae-derived marine oligosaccharides have been reported to be promising bioactive compounds because of their various properties with health benefits and potential significance in numerous applications in industrial biotechnology. In this study, laminaran oligosaccharides (LOs) with varying degrees...

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Autores principales: Cheong, Kit-Leong, Li, Jia-Kang, Zhong, Saiyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301192/
https://www.ncbi.nlm.nih.gov/pubmed/35873409
http://dx.doi.org/10.3389/fnut.2022.945804
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author Cheong, Kit-Leong
Li, Jia-Kang
Zhong, Saiyi
author_facet Cheong, Kit-Leong
Li, Jia-Kang
Zhong, Saiyi
author_sort Cheong, Kit-Leong
collection PubMed
description Algae-derived marine oligosaccharides have been reported to be promising bioactive compounds because of their various properties with health benefits and potential significance in numerous applications in industrial biotechnology. In this study, laminaran oligosaccharides (LOs) with varying degrees of polymerization were obtained through partial acid hydrolysis of laminaran derived from Laminaria digitata. Based on response surface methodology, the optimum LOs yield was obtained for acid hydrolysis laminaran at a hydrolysis time of 55 min, temperature of 71°C, and acid concentration of 1.00 mol/L. The size-exclusion resin Bio-Gel P-2 was considered to be a better option for LOs purification. The structure of the purified oligosaccharides was analyzed through mass spectrometry and nuclear magnetic resonance. They demonstrated the main oligosaccharide structure corresponding to the connection of glucose with β-D-Glcp-(1→3)-β-D-Glcp, which was identified as laminaribiose (DP2), laminaritriose (DP3), laminaritetrose (DP4), and laminaripentaose (DP5). LOs demonstrate excellent antioxidant activities, as evidenced from their reactions with oxidizing free radicals, 1, 1-diphenyl-2-picryl-hydrazyl, and 2, 2′-azino-bis (3-etilbenzotiazoline-6-sulfonic acid) radicals. LOs exhibited a prebiotic effect on the growth of Bifidobacterium adolescentis and Lactobacillus plantarum. Therefore, we propose the development of LOs as natural antioxidants and prebiotics in the functional food and pharmaceutical industries.
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spelling pubmed-93011922022-07-22 Preparation and Structure Characterization of High-Value Laminaria digitata Oligosaccharides Cheong, Kit-Leong Li, Jia-Kang Zhong, Saiyi Front Nutr Nutrition Algae-derived marine oligosaccharides have been reported to be promising bioactive compounds because of their various properties with health benefits and potential significance in numerous applications in industrial biotechnology. In this study, laminaran oligosaccharides (LOs) with varying degrees of polymerization were obtained through partial acid hydrolysis of laminaran derived from Laminaria digitata. Based on response surface methodology, the optimum LOs yield was obtained for acid hydrolysis laminaran at a hydrolysis time of 55 min, temperature of 71°C, and acid concentration of 1.00 mol/L. The size-exclusion resin Bio-Gel P-2 was considered to be a better option for LOs purification. The structure of the purified oligosaccharides was analyzed through mass spectrometry and nuclear magnetic resonance. They demonstrated the main oligosaccharide structure corresponding to the connection of glucose with β-D-Glcp-(1→3)-β-D-Glcp, which was identified as laminaribiose (DP2), laminaritriose (DP3), laminaritetrose (DP4), and laminaripentaose (DP5). LOs demonstrate excellent antioxidant activities, as evidenced from their reactions with oxidizing free radicals, 1, 1-diphenyl-2-picryl-hydrazyl, and 2, 2′-azino-bis (3-etilbenzotiazoline-6-sulfonic acid) radicals. LOs exhibited a prebiotic effect on the growth of Bifidobacterium adolescentis and Lactobacillus plantarum. Therefore, we propose the development of LOs as natural antioxidants and prebiotics in the functional food and pharmaceutical industries. Frontiers Media S.A. 2022-07-07 /pmc/articles/PMC9301192/ /pubmed/35873409 http://dx.doi.org/10.3389/fnut.2022.945804 Text en Copyright © 2022 Cheong, Li and Zhong. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Cheong, Kit-Leong
Li, Jia-Kang
Zhong, Saiyi
Preparation and Structure Characterization of High-Value Laminaria digitata Oligosaccharides
title Preparation and Structure Characterization of High-Value Laminaria digitata Oligosaccharides
title_full Preparation and Structure Characterization of High-Value Laminaria digitata Oligosaccharides
title_fullStr Preparation and Structure Characterization of High-Value Laminaria digitata Oligosaccharides
title_full_unstemmed Preparation and Structure Characterization of High-Value Laminaria digitata Oligosaccharides
title_short Preparation and Structure Characterization of High-Value Laminaria digitata Oligosaccharides
title_sort preparation and structure characterization of high-value laminaria digitata oligosaccharides
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301192/
https://www.ncbi.nlm.nih.gov/pubmed/35873409
http://dx.doi.org/10.3389/fnut.2022.945804
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