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Effect of Doxycycline Use in the Early Broiler Production Cycle on the Microbiome

16S rRNA amplicon sequencing was used to investigate changes in the broiler gastrointestinal tract (GIT) microbiota throughout the rearing period and in combination with antibiotic treatment. Thirty birds (from a commercial flock) were removed at multiple points throughout the rearing period on days...

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Autores principales: Greene, Genevieve, Koolman, Leonard, Whyte, Paul, Burgess, Catherine, Lynch, Helen, Coffey, Aidan, Lucey, Brigid, O’Connor, Lisa, Bolton, Declan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301238/
https://www.ncbi.nlm.nih.gov/pubmed/35875529
http://dx.doi.org/10.3389/fmicb.2022.885862
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author Greene, Genevieve
Koolman, Leonard
Whyte, Paul
Burgess, Catherine
Lynch, Helen
Coffey, Aidan
Lucey, Brigid
O’Connor, Lisa
Bolton, Declan
author_facet Greene, Genevieve
Koolman, Leonard
Whyte, Paul
Burgess, Catherine
Lynch, Helen
Coffey, Aidan
Lucey, Brigid
O’Connor, Lisa
Bolton, Declan
author_sort Greene, Genevieve
collection PubMed
description 16S rRNA amplicon sequencing was used to investigate changes in the broiler gastrointestinal tract (GIT) microbiota throughout the rearing period and in combination with antibiotic treatment. Thirty birds (from a commercial flock) were removed at multiple points throughout the rearing period on days 13, 27, and 33, euthanised, and their GIT aseptically removed and divided into upper (the crop, proventriculus, and the gizzard), middle (the duodenum, jejunum, and ileum) and lower (the large intestine, the caeca, and the cloaca) sections. In a separate commercial flock, on the same farm with similar husbandry practices and feed, doxycycline (100 mg/ml per kg body weight) was administered in drinking water between day 8 and 12 (inclusive) of the production cycle. Birds were removed on days, 13, 27, and 33 and GIT samples prepared as above. The contents of three merged samples from each GIT section were pooled (n = 60), the DNA extracted and analysed by 16S rRNA amplicon metagenomic sequencing and analysed. Major changes in the broiler microbiota were observed as the birds aged particularly with the Firmicutes/Bacteroidetes ratio (F:B) of the lower GIT. Moreover, Chao1, ACE, and Shannon indices showed the antibiotic treatment significantly altered the microbiota, and this change persisted throughout the rearing period. Further research is required to investigate the effect of these changes on bird performance, susceptibility to infections and Campylobacter carriage.
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spelling pubmed-93012382022-07-22 Effect of Doxycycline Use in the Early Broiler Production Cycle on the Microbiome Greene, Genevieve Koolman, Leonard Whyte, Paul Burgess, Catherine Lynch, Helen Coffey, Aidan Lucey, Brigid O’Connor, Lisa Bolton, Declan Front Microbiol Microbiology 16S rRNA amplicon sequencing was used to investigate changes in the broiler gastrointestinal tract (GIT) microbiota throughout the rearing period and in combination with antibiotic treatment. Thirty birds (from a commercial flock) were removed at multiple points throughout the rearing period on days 13, 27, and 33, euthanised, and their GIT aseptically removed and divided into upper (the crop, proventriculus, and the gizzard), middle (the duodenum, jejunum, and ileum) and lower (the large intestine, the caeca, and the cloaca) sections. In a separate commercial flock, on the same farm with similar husbandry practices and feed, doxycycline (100 mg/ml per kg body weight) was administered in drinking water between day 8 and 12 (inclusive) of the production cycle. Birds were removed on days, 13, 27, and 33 and GIT samples prepared as above. The contents of three merged samples from each GIT section were pooled (n = 60), the DNA extracted and analysed by 16S rRNA amplicon metagenomic sequencing and analysed. Major changes in the broiler microbiota were observed as the birds aged particularly with the Firmicutes/Bacteroidetes ratio (F:B) of the lower GIT. Moreover, Chao1, ACE, and Shannon indices showed the antibiotic treatment significantly altered the microbiota, and this change persisted throughout the rearing period. Further research is required to investigate the effect of these changes on bird performance, susceptibility to infections and Campylobacter carriage. Frontiers Media S.A. 2022-07-07 /pmc/articles/PMC9301238/ /pubmed/35875529 http://dx.doi.org/10.3389/fmicb.2022.885862 Text en Copyright © 2022 Greene, Koolman, Whyte, Burgess, Lynch, Coffey, Lucey, O’Connor and Bolton. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Greene, Genevieve
Koolman, Leonard
Whyte, Paul
Burgess, Catherine
Lynch, Helen
Coffey, Aidan
Lucey, Brigid
O’Connor, Lisa
Bolton, Declan
Effect of Doxycycline Use in the Early Broiler Production Cycle on the Microbiome
title Effect of Doxycycline Use in the Early Broiler Production Cycle on the Microbiome
title_full Effect of Doxycycline Use in the Early Broiler Production Cycle on the Microbiome
title_fullStr Effect of Doxycycline Use in the Early Broiler Production Cycle on the Microbiome
title_full_unstemmed Effect of Doxycycline Use in the Early Broiler Production Cycle on the Microbiome
title_short Effect of Doxycycline Use in the Early Broiler Production Cycle on the Microbiome
title_sort effect of doxycycline use in the early broiler production cycle on the microbiome
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301238/
https://www.ncbi.nlm.nih.gov/pubmed/35875529
http://dx.doi.org/10.3389/fmicb.2022.885862
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