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Wound-Healing and Antibacterial Activity of the Quercetin–4-Formyl Phenyl Boronic Acid Complex against Bacterial Pathogens of Diabetic Foot Ulcer

[Image: see text] Complications of diabetic foot can be prevented using a naturally occurring, efficient, and newly synthesized antimicrobial agent. The purpose of the study was to improve wound healing and antibacterial effects of quercetin and its esterified complex with 4-formyl phenyl boronic ac...

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Autores principales: Abid, Hafiz Muhammad Usman, Hanif, Muhammad, Mahmood, Khalid, Aziz, Mubashir, Abbas, Ghulam, Latif, Hafsa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301639/
https://www.ncbi.nlm.nih.gov/pubmed/35874257
http://dx.doi.org/10.1021/acsomega.2c01819
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author Abid, Hafiz Muhammad Usman
Hanif, Muhammad
Mahmood, Khalid
Aziz, Mubashir
Abbas, Ghulam
Latif, Hafsa
author_facet Abid, Hafiz Muhammad Usman
Hanif, Muhammad
Mahmood, Khalid
Aziz, Mubashir
Abbas, Ghulam
Latif, Hafsa
author_sort Abid, Hafiz Muhammad Usman
collection PubMed
description [Image: see text] Complications of diabetic foot can be prevented using a naturally occurring, efficient, and newly synthesized antimicrobial agent. The purpose of the study was to improve wound healing and antibacterial effects of quercetin and its esterified complex with 4-formyl phenyl boronic acid (4FPBA–Q) compared with phenytoin. The formation of the 4FPBA–Q complex was confirmed by thin-layer chromatography (TLC), Fourier transform infrared (FTIR) spectroscopy, and mass spectrometry (MS). The prepared 4FPBA–Q complex was used against Gram-positive bacteria along with Gram-negative bacteria, and more than 2-fold decrease in minimum inhibitory concentrations (MIC) was observed compared to pure quercetin. Scanning electron microscopic images of Pseudomonas aeruginosa and Staphylococcus aureus showed their complete destruction after incubation with the 4FPBA–Q complex even after 3 h. Interestingly, wound-healing properties of the 4FPBA–Q complex in infected diabetic rats increased from 64 to 99% as compared to phenytoin, which were increased from those of noninfected diabetic rats. Furthermore, histopathological evaluations showed significantly enhanced wound healing, re-epithelialization, fibroblasts, and angiogenesis in wounds of diabetic rats after 10 days. Conclusively, reduction in the primary irritation index (PDII) and improved antibacterial and wound-healing properties render the 4FPBA–Q complex ideal for diabetic foot ulcer treatment.
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spelling pubmed-93016392022-07-22 Wound-Healing and Antibacterial Activity of the Quercetin–4-Formyl Phenyl Boronic Acid Complex against Bacterial Pathogens of Diabetic Foot Ulcer Abid, Hafiz Muhammad Usman Hanif, Muhammad Mahmood, Khalid Aziz, Mubashir Abbas, Ghulam Latif, Hafsa ACS Omega [Image: see text] Complications of diabetic foot can be prevented using a naturally occurring, efficient, and newly synthesized antimicrobial agent. The purpose of the study was to improve wound healing and antibacterial effects of quercetin and its esterified complex with 4-formyl phenyl boronic acid (4FPBA–Q) compared with phenytoin. The formation of the 4FPBA–Q complex was confirmed by thin-layer chromatography (TLC), Fourier transform infrared (FTIR) spectroscopy, and mass spectrometry (MS). The prepared 4FPBA–Q complex was used against Gram-positive bacteria along with Gram-negative bacteria, and more than 2-fold decrease in minimum inhibitory concentrations (MIC) was observed compared to pure quercetin. Scanning electron microscopic images of Pseudomonas aeruginosa and Staphylococcus aureus showed their complete destruction after incubation with the 4FPBA–Q complex even after 3 h. Interestingly, wound-healing properties of the 4FPBA–Q complex in infected diabetic rats increased from 64 to 99% as compared to phenytoin, which were increased from those of noninfected diabetic rats. Furthermore, histopathological evaluations showed significantly enhanced wound healing, re-epithelialization, fibroblasts, and angiogenesis in wounds of diabetic rats after 10 days. Conclusively, reduction in the primary irritation index (PDII) and improved antibacterial and wound-healing properties render the 4FPBA–Q complex ideal for diabetic foot ulcer treatment. American Chemical Society 2022-07-08 /pmc/articles/PMC9301639/ /pubmed/35874257 http://dx.doi.org/10.1021/acsomega.2c01819 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Abid, Hafiz Muhammad Usman
Hanif, Muhammad
Mahmood, Khalid
Aziz, Mubashir
Abbas, Ghulam
Latif, Hafsa
Wound-Healing and Antibacterial Activity of the Quercetin–4-Formyl Phenyl Boronic Acid Complex against Bacterial Pathogens of Diabetic Foot Ulcer
title Wound-Healing and Antibacterial Activity of the Quercetin–4-Formyl Phenyl Boronic Acid Complex against Bacterial Pathogens of Diabetic Foot Ulcer
title_full Wound-Healing and Antibacterial Activity of the Quercetin–4-Formyl Phenyl Boronic Acid Complex against Bacterial Pathogens of Diabetic Foot Ulcer
title_fullStr Wound-Healing and Antibacterial Activity of the Quercetin–4-Formyl Phenyl Boronic Acid Complex against Bacterial Pathogens of Diabetic Foot Ulcer
title_full_unstemmed Wound-Healing and Antibacterial Activity of the Quercetin–4-Formyl Phenyl Boronic Acid Complex against Bacterial Pathogens of Diabetic Foot Ulcer
title_short Wound-Healing and Antibacterial Activity of the Quercetin–4-Formyl Phenyl Boronic Acid Complex against Bacterial Pathogens of Diabetic Foot Ulcer
title_sort wound-healing and antibacterial activity of the quercetin–4-formyl phenyl boronic acid complex against bacterial pathogens of diabetic foot ulcer
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301639/
https://www.ncbi.nlm.nih.gov/pubmed/35874257
http://dx.doi.org/10.1021/acsomega.2c01819
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