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A CMV-induced adaptive human Vδ1(+) γδ T cell clone recognizes HLA-DR
The innate and adaptive roles of γδ T cells and their clonal γδ T cell receptors (TCRs) in immune responses are still unclear. Recent studies of γδ TCR repertoire dynamics showed massive expansion of individual Vδ1(+) γδ T cell clones during viral infection. To judge whether such expansion is random...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301659/ https://www.ncbi.nlm.nih.gov/pubmed/35852466 http://dx.doi.org/10.1084/jem.20212525 |
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author | Deseke, Malte Rampoldi, Francesca Sandrock, Inga Borst, Eva Böning, Heike Ssebyatika, George Liam Jürgens, Carina Plückebaum, Nina Beck, Maleen Hassan, Ahmed Tan, Likai Demera, Abdi Janssen, Anika Steinberger, Peter Koenecke, Christian Viejo-Borbolla, Abel Messerle, Martin Krey, Thomas Prinz, Immo |
author_facet | Deseke, Malte Rampoldi, Francesca Sandrock, Inga Borst, Eva Böning, Heike Ssebyatika, George Liam Jürgens, Carina Plückebaum, Nina Beck, Maleen Hassan, Ahmed Tan, Likai Demera, Abdi Janssen, Anika Steinberger, Peter Koenecke, Christian Viejo-Borbolla, Abel Messerle, Martin Krey, Thomas Prinz, Immo |
author_sort | Deseke, Malte |
collection | PubMed |
description | The innate and adaptive roles of γδ T cells and their clonal γδ T cell receptors (TCRs) in immune responses are still unclear. Recent studies of γδ TCR repertoire dynamics showed massive expansion of individual Vδ1(+) γδ T cell clones during viral infection. To judge whether such expansion is random or actually represents TCR-dependent adaptive immune responses, information about their cognate TCR ligands is required. Here, we used CRISPR/Cas9-mediated screening to identify HLA-DRA, RFXAP, RFX5, and CIITA as required for target cell recognition of a CMV-induced Vγ3Vδ1(+) TCR, and further characterization revealed a direct interaction of this Vδ1(+) TCR with the MHC II complex HLA-DR. Since MHC II is strongly upregulated by interferon-γ, these results suggest an inflammation-induced MHC-dependent immune response of γδ T cells. |
format | Online Article Text |
id | pubmed-9301659 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-93016592023-01-19 A CMV-induced adaptive human Vδ1(+) γδ T cell clone recognizes HLA-DR Deseke, Malte Rampoldi, Francesca Sandrock, Inga Borst, Eva Böning, Heike Ssebyatika, George Liam Jürgens, Carina Plückebaum, Nina Beck, Maleen Hassan, Ahmed Tan, Likai Demera, Abdi Janssen, Anika Steinberger, Peter Koenecke, Christian Viejo-Borbolla, Abel Messerle, Martin Krey, Thomas Prinz, Immo J Exp Med Brief Definitive Report The innate and adaptive roles of γδ T cells and their clonal γδ T cell receptors (TCRs) in immune responses are still unclear. Recent studies of γδ TCR repertoire dynamics showed massive expansion of individual Vδ1(+) γδ T cell clones during viral infection. To judge whether such expansion is random or actually represents TCR-dependent adaptive immune responses, information about their cognate TCR ligands is required. Here, we used CRISPR/Cas9-mediated screening to identify HLA-DRA, RFXAP, RFX5, and CIITA as required for target cell recognition of a CMV-induced Vγ3Vδ1(+) TCR, and further characterization revealed a direct interaction of this Vδ1(+) TCR with the MHC II complex HLA-DR. Since MHC II is strongly upregulated by interferon-γ, these results suggest an inflammation-induced MHC-dependent immune response of γδ T cells. Rockefeller University Press 2022-07-19 /pmc/articles/PMC9301659/ /pubmed/35852466 http://dx.doi.org/10.1084/jem.20212525 Text en © 2022 Deseke et al. https://creativecommons.org/licenses/by-nc-sa/4.0/http://www.rupress.org/terms/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Brief Definitive Report Deseke, Malte Rampoldi, Francesca Sandrock, Inga Borst, Eva Böning, Heike Ssebyatika, George Liam Jürgens, Carina Plückebaum, Nina Beck, Maleen Hassan, Ahmed Tan, Likai Demera, Abdi Janssen, Anika Steinberger, Peter Koenecke, Christian Viejo-Borbolla, Abel Messerle, Martin Krey, Thomas Prinz, Immo A CMV-induced adaptive human Vδ1(+) γδ T cell clone recognizes HLA-DR |
title | A CMV-induced adaptive human Vδ1(+) γδ T cell clone recognizes HLA-DR |
title_full | A CMV-induced adaptive human Vδ1(+) γδ T cell clone recognizes HLA-DR |
title_fullStr | A CMV-induced adaptive human Vδ1(+) γδ T cell clone recognizes HLA-DR |
title_full_unstemmed | A CMV-induced adaptive human Vδ1(+) γδ T cell clone recognizes HLA-DR |
title_short | A CMV-induced adaptive human Vδ1(+) γδ T cell clone recognizes HLA-DR |
title_sort | cmv-induced adaptive human vδ1(+) γδ t cell clone recognizes hla-dr |
topic | Brief Definitive Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301659/ https://www.ncbi.nlm.nih.gov/pubmed/35852466 http://dx.doi.org/10.1084/jem.20212525 |
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