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Role of cryopreserved multipotent mesenchymal stromal cells in modulation of some indices of cell immunity in adjuvant arthritis

INRODUCTION: The results of experimental and clinical studies in recent years indicate that the transplantation of multipotent mesenchymal stromal cells (MMSCs) is a possible approach for the “restoration” of the immune system of patients with autoimmune diseases, in particular, rheumatoid arthritis...

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Autores principales: Vvedenskyi, Dmytro, Volkova, Nataliia, Babenko, Natalya, Gaevska, Yulia, Yukhta, Mariia, Goltsev, Anatoliy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Narodowy Instytut Geriatrii, Reumatologii i Rehabilitacji w Warszawie 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301669/
https://www.ncbi.nlm.nih.gov/pubmed/35875720
http://dx.doi.org/10.5114/reum.2022.117842
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author Vvedenskyi, Dmytro
Volkova, Nataliia
Babenko, Natalya
Gaevska, Yulia
Yukhta, Mariia
Goltsev, Anatoliy
author_facet Vvedenskyi, Dmytro
Volkova, Nataliia
Babenko, Natalya
Gaevska, Yulia
Yukhta, Mariia
Goltsev, Anatoliy
author_sort Vvedenskyi, Dmytro
collection PubMed
description INRODUCTION: The results of experimental and clinical studies in recent years indicate that the transplantation of multipotent mesenchymal stromal cells (MMSCs) is a possible approach for the “restoration” of the immune system of patients with autoimmune diseases, in particular, rheumatoid arthritis. However, the strength and duration of the effect vary greatly, which indicates incomplete correction of the tested parameters, thereby opening up the prospect of improving this method of treatment by choosing dose-time parameters and methods of their administration. The aim of this research was to determine the indices of cellular immunity in animals with adjuvant arthritis and therapy with cryopreserved MMSCs derived from adipose and cartilage tissues. MATERIAL AND METHODS: Adjuvant arthritis in male rats was modeled by subplantar administration of Freund’s complete adjuvant. On day 7 of modeling, experimental animals were administered with saline (control group) or cryopreserved MMSCs from adipose or cartilaginous tissue locally or generalized. On day 28 after therapy the body weight, spleen index and cellularity, and content of CD3+, CD4+, CD8+, CD4+CD25+ cells in the spleen were determined. RESULTS: In the control group of animals, the inflammation was pronounced, as evidenced by a significant increase in the studied parameters throughout the observation period. The use of cryopreserved MMSCs from adipose and cartilaginous tissues led to the restoration of T regulatory cells (Treg) on day 28. Generalized administration of cells had a more pronounced therapeutic effect compared to the animals with local administration. These data can be used to justify and develop a therapeutic approach to rheumatoid arthritis in clinical practice. CONCLUSIONS: Cell therapy with cryopreserved MMSCs from investigated sources provided by both local and generalized administration to animals with adjuvant arthritis has a correcting effect on the cellular immunity.
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spelling pubmed-93016692022-07-22 Role of cryopreserved multipotent mesenchymal stromal cells in modulation of some indices of cell immunity in adjuvant arthritis Vvedenskyi, Dmytro Volkova, Nataliia Babenko, Natalya Gaevska, Yulia Yukhta, Mariia Goltsev, Anatoliy Reumatologia Original Paper INRODUCTION: The results of experimental and clinical studies in recent years indicate that the transplantation of multipotent mesenchymal stromal cells (MMSCs) is a possible approach for the “restoration” of the immune system of patients with autoimmune diseases, in particular, rheumatoid arthritis. However, the strength and duration of the effect vary greatly, which indicates incomplete correction of the tested parameters, thereby opening up the prospect of improving this method of treatment by choosing dose-time parameters and methods of their administration. The aim of this research was to determine the indices of cellular immunity in animals with adjuvant arthritis and therapy with cryopreserved MMSCs derived from adipose and cartilage tissues. MATERIAL AND METHODS: Adjuvant arthritis in male rats was modeled by subplantar administration of Freund’s complete adjuvant. On day 7 of modeling, experimental animals were administered with saline (control group) or cryopreserved MMSCs from adipose or cartilaginous tissue locally or generalized. On day 28 after therapy the body weight, spleen index and cellularity, and content of CD3+, CD4+, CD8+, CD4+CD25+ cells in the spleen were determined. RESULTS: In the control group of animals, the inflammation was pronounced, as evidenced by a significant increase in the studied parameters throughout the observation period. The use of cryopreserved MMSCs from adipose and cartilaginous tissues led to the restoration of T regulatory cells (Treg) on day 28. Generalized administration of cells had a more pronounced therapeutic effect compared to the animals with local administration. These data can be used to justify and develop a therapeutic approach to rheumatoid arthritis in clinical practice. CONCLUSIONS: Cell therapy with cryopreserved MMSCs from investigated sources provided by both local and generalized administration to animals with adjuvant arthritis has a correcting effect on the cellular immunity. Narodowy Instytut Geriatrii, Reumatologii i Rehabilitacji w Warszawie 2022-07-13 2022 /pmc/articles/PMC9301669/ /pubmed/35875720 http://dx.doi.org/10.5114/reum.2022.117842 Text en Copyright: © 2022 Narodowy Instytut Geriatrii, Reumatologii i Rehabilitacji w Warszawie https://creativecommons.org/licenses/by-nc-sa/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
spellingShingle Original Paper
Vvedenskyi, Dmytro
Volkova, Nataliia
Babenko, Natalya
Gaevska, Yulia
Yukhta, Mariia
Goltsev, Anatoliy
Role of cryopreserved multipotent mesenchymal stromal cells in modulation of some indices of cell immunity in adjuvant arthritis
title Role of cryopreserved multipotent mesenchymal stromal cells in modulation of some indices of cell immunity in adjuvant arthritis
title_full Role of cryopreserved multipotent mesenchymal stromal cells in modulation of some indices of cell immunity in adjuvant arthritis
title_fullStr Role of cryopreserved multipotent mesenchymal stromal cells in modulation of some indices of cell immunity in adjuvant arthritis
title_full_unstemmed Role of cryopreserved multipotent mesenchymal stromal cells in modulation of some indices of cell immunity in adjuvant arthritis
title_short Role of cryopreserved multipotent mesenchymal stromal cells in modulation of some indices of cell immunity in adjuvant arthritis
title_sort role of cryopreserved multipotent mesenchymal stromal cells in modulation of some indices of cell immunity in adjuvant arthritis
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301669/
https://www.ncbi.nlm.nih.gov/pubmed/35875720
http://dx.doi.org/10.5114/reum.2022.117842
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