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Effects of Exercise Training on PPARβ/δ Expression in Skeletal Muscle of Rats with Spontaneous Hypertension

PURPOSE: This study aimed to identify the relationship and mechanism between skeletal muscle peroxisome proliferator–activated receptor β/δ (PPARβ/δ) and spontaneous hypertension. METHODS: Rats were divided into four groups (n = 10): spontaneous hypertensive rats exercise group (SHR-E), spontaneous...

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Autores principales: YANG, MINGXUAN, PAN, YANXIA, LI, KUNHUI, CHEN, XIUYUN, LI, MINYAN, LIN, JIANPING, LI, MING, LIN, CHENG
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301982/
https://www.ncbi.nlm.nih.gov/pubmed/35389955
http://dx.doi.org/10.1249/MSS.0000000000002925
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author YANG, MINGXUAN
PAN, YANXIA
LI, KUNHUI
CHEN, XIUYUN
LI, MINYAN
LIN, JIANPING
LI, MING
LIN, CHENG
author_facet YANG, MINGXUAN
PAN, YANXIA
LI, KUNHUI
CHEN, XIUYUN
LI, MINYAN
LIN, JIANPING
LI, MING
LIN, CHENG
author_sort YANG, MINGXUAN
collection PubMed
description PURPOSE: This study aimed to identify the relationship and mechanism between skeletal muscle peroxisome proliferator–activated receptor β/δ (PPARβ/δ) and spontaneous hypertension. METHODS: Rats were divided into four groups (n = 10): spontaneous hypertensive rats exercise group (SHR-E), spontaneous hypertensive rats sedentary group (SHR-S), Wistar-Kyoto control rats exercise group (WKY-E), and Wistar-Kyoto control rats sedentary group (WKY-S). Although the sedentary groups were placed on the treadmill without moving during the training sessions, the exercise groups were forced to run on a treadmill for 8 wk, 1 h·d(−1), 5 d·wk(−1). After training, the density and area of gastrocnemius microvessels were observed. PPARβ/δ, vascular endothelial growth factor A (VEGFA), superoxide dismutase 2 (SOD-2), and nitric oxide synthase in gastrocnemius were measured by real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot. RESULTS: Except the sixth week of age, the systolic blood pressure of SHR-S was significantly higher than that of WKY-S at all time periods. Exercise significantly reduced systolic blood pressure in SHR rats. Compared with the SHR-S group, the WKY-S group had significantly higher PPARβ/δ protein level and density of skeletal muscle microvessels. Eight weeks of exercise increased the PPARβ/δ, SOD-2, VEGFA, and microvessel density and area in the skeletal muscle of SHR. CONCLUSIONS: Exercise training promoted PPARβ/δ mRNA and protein-level expression of PPARβ/δ, SOD-2 and VEGFA in skeletal muscle, thus increasing the density and area of skeletal muscle blood vessels. These regulations contribute to the reduction of peripheral vascular resistance. This may be a potential mechanism of exercise to reduce blood pressure.
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spelling pubmed-93019822022-08-02 Effects of Exercise Training on PPARβ/δ Expression in Skeletal Muscle of Rats with Spontaneous Hypertension YANG, MINGXUAN PAN, YANXIA LI, KUNHUI CHEN, XIUYUN LI, MINYAN LIN, JIANPING LI, MING LIN, CHENG Med Sci Sports Exerc Basic Sciences PURPOSE: This study aimed to identify the relationship and mechanism between skeletal muscle peroxisome proliferator–activated receptor β/δ (PPARβ/δ) and spontaneous hypertension. METHODS: Rats were divided into four groups (n = 10): spontaneous hypertensive rats exercise group (SHR-E), spontaneous hypertensive rats sedentary group (SHR-S), Wistar-Kyoto control rats exercise group (WKY-E), and Wistar-Kyoto control rats sedentary group (WKY-S). Although the sedentary groups were placed on the treadmill without moving during the training sessions, the exercise groups were forced to run on a treadmill for 8 wk, 1 h·d(−1), 5 d·wk(−1). After training, the density and area of gastrocnemius microvessels were observed. PPARβ/δ, vascular endothelial growth factor A (VEGFA), superoxide dismutase 2 (SOD-2), and nitric oxide synthase in gastrocnemius were measured by real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot. RESULTS: Except the sixth week of age, the systolic blood pressure of SHR-S was significantly higher than that of WKY-S at all time periods. Exercise significantly reduced systolic blood pressure in SHR rats. Compared with the SHR-S group, the WKY-S group had significantly higher PPARβ/δ protein level and density of skeletal muscle microvessels. Eight weeks of exercise increased the PPARβ/δ, SOD-2, VEGFA, and microvessel density and area in the skeletal muscle of SHR. CONCLUSIONS: Exercise training promoted PPARβ/δ mRNA and protein-level expression of PPARβ/δ, SOD-2 and VEGFA in skeletal muscle, thus increasing the density and area of skeletal muscle blood vessels. These regulations contribute to the reduction of peripheral vascular resistance. This may be a potential mechanism of exercise to reduce blood pressure. Lippincott Williams & Wilkins 2022-08 2022-04-02 /pmc/articles/PMC9301982/ /pubmed/35389955 http://dx.doi.org/10.1249/MSS.0000000000002925 Text en Copyright © 2022 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American College of Sports Medicine. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) , where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.
spellingShingle Basic Sciences
YANG, MINGXUAN
PAN, YANXIA
LI, KUNHUI
CHEN, XIUYUN
LI, MINYAN
LIN, JIANPING
LI, MING
LIN, CHENG
Effects of Exercise Training on PPARβ/δ Expression in Skeletal Muscle of Rats with Spontaneous Hypertension
title Effects of Exercise Training on PPARβ/δ Expression in Skeletal Muscle of Rats with Spontaneous Hypertension
title_full Effects of Exercise Training on PPARβ/δ Expression in Skeletal Muscle of Rats with Spontaneous Hypertension
title_fullStr Effects of Exercise Training on PPARβ/δ Expression in Skeletal Muscle of Rats with Spontaneous Hypertension
title_full_unstemmed Effects of Exercise Training on PPARβ/δ Expression in Skeletal Muscle of Rats with Spontaneous Hypertension
title_short Effects of Exercise Training on PPARβ/δ Expression in Skeletal Muscle of Rats with Spontaneous Hypertension
title_sort effects of exercise training on pparβ/δ expression in skeletal muscle of rats with spontaneous hypertension
topic Basic Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301982/
https://www.ncbi.nlm.nih.gov/pubmed/35389955
http://dx.doi.org/10.1249/MSS.0000000000002925
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