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Survey of tick species and molecular detection of selected tick-borne pathogens in Yanbian, China
Ticks and tick-borne diseases pose a significant threat to public health. In this study, we aimed to determine the tick species distribution and pathogens carried by ticks in Yanbian, China. A total of 2673 questing ticks were collected from eight counties and cities in Yanbian and were morphologica...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
EDP Sciences
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302104/ https://www.ncbi.nlm.nih.gov/pubmed/35861542 http://dx.doi.org/10.1051/parasite/2022039 |
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author | Li, Jixu Zhang, Shuang Liang, Wanfeng Zhao, Shaowei Wang, Zhenyu Li, Hang Yang, Bingyi Zhang, Zhen Li, Jialin Jia, Lijun |
author_facet | Li, Jixu Zhang, Shuang Liang, Wanfeng Zhao, Shaowei Wang, Zhenyu Li, Hang Yang, Bingyi Zhang, Zhen Li, Jialin Jia, Lijun |
author_sort | Li, Jixu |
collection | PubMed |
description | Ticks and tick-borne diseases pose a significant threat to public health. In this study, we aimed to determine the tick species distribution and pathogens carried by ticks in Yanbian, China. A total of 2673 questing ticks were collected from eight counties and cities in Yanbian and were morphologically identified. The presence of Candidatus Rickettsia tarasevichiae (CRT), spotted fever group Rickettsia (SFGR), severe fever thrombocytopenia syndrome virus (SFTSV), Theileria, and other pathogens was confirmed using polymerase chain reaction (PCR) and real-time quantitative PCR assays, followed by phylogenetic and genotypic analyses. According to the morphological identification, the tick species in Yanbian consisted of Haemaphysalis longicornis, Ixodes persulcatus, Dermacentor silvarum, H. japonica, and H. concinna. In H. longicornis, CRT, SFGR, SFTSV and Theileria orientalis were detected, while CRT, SFGR, and SFTSV were detected in I. persulcatus, H. japonica, and D. silvarum. Only SFTSV was detected in H. concinna. Mixed infection with CRT and SFTSV was observed in I. persulcatus and H. japonica. The gene sequences of all tested pathogens exhibited 95.7%–100% identity with the corresponding sequences deposited in GenBank. Phylogenetic analysis showed that different SFGR and SFTSV genotypes were closely related to the Korean strains. This study is the first to describe the genetic diversity of SFGR Candidatus Rickettsia longicornii in H. longicornis in Yanbian, China, using the ompA, ompB, sca4, and rrs genes. These results provide epidemiological data to support the prevention and control of ticks and tick-borne diseases in the border areas of China, North Korea, and Russia. |
format | Online Article Text |
id | pubmed-9302104 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | EDP Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-93021042022-08-01 Survey of tick species and molecular detection of selected tick-borne pathogens in Yanbian, China Li, Jixu Zhang, Shuang Liang, Wanfeng Zhao, Shaowei Wang, Zhenyu Li, Hang Yang, Bingyi Zhang, Zhen Li, Jialin Jia, Lijun Parasite Research Article Ticks and tick-borne diseases pose a significant threat to public health. In this study, we aimed to determine the tick species distribution and pathogens carried by ticks in Yanbian, China. A total of 2673 questing ticks were collected from eight counties and cities in Yanbian and were morphologically identified. The presence of Candidatus Rickettsia tarasevichiae (CRT), spotted fever group Rickettsia (SFGR), severe fever thrombocytopenia syndrome virus (SFTSV), Theileria, and other pathogens was confirmed using polymerase chain reaction (PCR) and real-time quantitative PCR assays, followed by phylogenetic and genotypic analyses. According to the morphological identification, the tick species in Yanbian consisted of Haemaphysalis longicornis, Ixodes persulcatus, Dermacentor silvarum, H. japonica, and H. concinna. In H. longicornis, CRT, SFGR, SFTSV and Theileria orientalis were detected, while CRT, SFGR, and SFTSV were detected in I. persulcatus, H. japonica, and D. silvarum. Only SFTSV was detected in H. concinna. Mixed infection with CRT and SFTSV was observed in I. persulcatus and H. japonica. The gene sequences of all tested pathogens exhibited 95.7%–100% identity with the corresponding sequences deposited in GenBank. Phylogenetic analysis showed that different SFGR and SFTSV genotypes were closely related to the Korean strains. This study is the first to describe the genetic diversity of SFGR Candidatus Rickettsia longicornii in H. longicornis in Yanbian, China, using the ompA, ompB, sca4, and rrs genes. These results provide epidemiological data to support the prevention and control of ticks and tick-borne diseases in the border areas of China, North Korea, and Russia. EDP Sciences 2022-07-21 /pmc/articles/PMC9302104/ /pubmed/35861542 http://dx.doi.org/10.1051/parasite/2022039 Text en © J. Li et al., published by EDP Sciences, 2022 https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Li, Jixu Zhang, Shuang Liang, Wanfeng Zhao, Shaowei Wang, Zhenyu Li, Hang Yang, Bingyi Zhang, Zhen Li, Jialin Jia, Lijun Survey of tick species and molecular detection of selected tick-borne pathogens in Yanbian, China |
title | Survey of tick species and molecular detection of selected tick-borne pathogens in Yanbian, China |
title_full | Survey of tick species and molecular detection of selected tick-borne pathogens in Yanbian, China |
title_fullStr | Survey of tick species and molecular detection of selected tick-borne pathogens in Yanbian, China |
title_full_unstemmed | Survey of tick species and molecular detection of selected tick-borne pathogens in Yanbian, China |
title_short | Survey of tick species and molecular detection of selected tick-borne pathogens in Yanbian, China |
title_sort | survey of tick species and molecular detection of selected tick-borne pathogens in yanbian, china |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302104/ https://www.ncbi.nlm.nih.gov/pubmed/35861542 http://dx.doi.org/10.1051/parasite/2022039 |
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