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Detection of neutralizing antibodies against multiple SARS-CoV-2 strains in dried blood spots using cell-free PCR
An easily implementable serological assay to accurately detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) neutralizing antibodies is urgently needed to better track herd immunity, vaccine efficacy and vaccination rates. Herein, we report the Split-Oligonucleotide Neighboring Inhibi...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302227/ https://www.ncbi.nlm.nih.gov/pubmed/35864104 http://dx.doi.org/10.1038/s41467-022-31796-1 |
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| author | Danh, Kenneth Karp, Donna Grace Singhal, Malvika Tankasala, Akshaya Gebhart, David de Jesus Cortez, Felipe Tandel, Devangkumar Robinson, Peter V. Seftel, David Stone, Mars Simmons, Graham Bagri, Anil Schreiber, Martin A. Buser, Andreas Holbro, Andreas Battegay, Manuel Morris, Mary Kate Hanson, Carl Mills, John R. Granger, Dane Theel, Elitza S. Stubbs, James R. Corash, Laurence M. Tsai, Cheng-ting |
| author_facet | Danh, Kenneth Karp, Donna Grace Singhal, Malvika Tankasala, Akshaya Gebhart, David de Jesus Cortez, Felipe Tandel, Devangkumar Robinson, Peter V. Seftel, David Stone, Mars Simmons, Graham Bagri, Anil Schreiber, Martin A. Buser, Andreas Holbro, Andreas Battegay, Manuel Morris, Mary Kate Hanson, Carl Mills, John R. Granger, Dane Theel, Elitza S. Stubbs, James R. Corash, Laurence M. Tsai, Cheng-ting |
| author_sort | Danh, Kenneth |
| collection | PubMed |
| description | An easily implementable serological assay to accurately detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) neutralizing antibodies is urgently needed to better track herd immunity, vaccine efficacy and vaccination rates. Herein, we report the Split-Oligonucleotide Neighboring Inhibition Assay (SONIA) which uses real-time qPCR to measure the ability of neutralizing antibodies to block binding between DNA-barcoded viral spike protein subunit 1 and the human angiotensin-converting enzyme 2 receptor protein. The SONIA neutralizing antibody assay using finger-prick dried blood spots displays 91–97% sensitivity and 100% specificity in comparison to the live-virus neutralization assays using matched serum specimens for multiple SARS-CoV-2 variants-of-concern. The multiplex version of this neutralizing antibody assay, using easily collectable finger-prick dried blood spots, can be a valuable tool to help reveal the impact of age, pre-existing health conditions, waning immunity, different vaccination schemes and the emergence of new variants-of-concern. |
| format | Online Article Text |
| id | pubmed-9302227 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2022 |
| publisher | Nature Publishing Group UK |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-93022272022-07-22 Detection of neutralizing antibodies against multiple SARS-CoV-2 strains in dried blood spots using cell-free PCR Danh, Kenneth Karp, Donna Grace Singhal, Malvika Tankasala, Akshaya Gebhart, David de Jesus Cortez, Felipe Tandel, Devangkumar Robinson, Peter V. Seftel, David Stone, Mars Simmons, Graham Bagri, Anil Schreiber, Martin A. Buser, Andreas Holbro, Andreas Battegay, Manuel Morris, Mary Kate Hanson, Carl Mills, John R. Granger, Dane Theel, Elitza S. Stubbs, James R. Corash, Laurence M. Tsai, Cheng-ting Nat Commun Article An easily implementable serological assay to accurately detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) neutralizing antibodies is urgently needed to better track herd immunity, vaccine efficacy and vaccination rates. Herein, we report the Split-Oligonucleotide Neighboring Inhibition Assay (SONIA) which uses real-time qPCR to measure the ability of neutralizing antibodies to block binding between DNA-barcoded viral spike protein subunit 1 and the human angiotensin-converting enzyme 2 receptor protein. The SONIA neutralizing antibody assay using finger-prick dried blood spots displays 91–97% sensitivity and 100% specificity in comparison to the live-virus neutralization assays using matched serum specimens for multiple SARS-CoV-2 variants-of-concern. The multiplex version of this neutralizing antibody assay, using easily collectable finger-prick dried blood spots, can be a valuable tool to help reveal the impact of age, pre-existing health conditions, waning immunity, different vaccination schemes and the emergence of new variants-of-concern. Nature Publishing Group UK 2022-07-21 /pmc/articles/PMC9302227/ /pubmed/35864104 http://dx.doi.org/10.1038/s41467-022-31796-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
| spellingShingle | Article Danh, Kenneth Karp, Donna Grace Singhal, Malvika Tankasala, Akshaya Gebhart, David de Jesus Cortez, Felipe Tandel, Devangkumar Robinson, Peter V. Seftel, David Stone, Mars Simmons, Graham Bagri, Anil Schreiber, Martin A. Buser, Andreas Holbro, Andreas Battegay, Manuel Morris, Mary Kate Hanson, Carl Mills, John R. Granger, Dane Theel, Elitza S. Stubbs, James R. Corash, Laurence M. Tsai, Cheng-ting Detection of neutralizing antibodies against multiple SARS-CoV-2 strains in dried blood spots using cell-free PCR |
| title | Detection of neutralizing antibodies against multiple SARS-CoV-2 strains in dried blood spots using cell-free PCR |
| title_full | Detection of neutralizing antibodies against multiple SARS-CoV-2 strains in dried blood spots using cell-free PCR |
| title_fullStr | Detection of neutralizing antibodies against multiple SARS-CoV-2 strains in dried blood spots using cell-free PCR |
| title_full_unstemmed | Detection of neutralizing antibodies against multiple SARS-CoV-2 strains in dried blood spots using cell-free PCR |
| title_short | Detection of neutralizing antibodies against multiple SARS-CoV-2 strains in dried blood spots using cell-free PCR |
| title_sort | detection of neutralizing antibodies against multiple sars-cov-2 strains in dried blood spots using cell-free pcr |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302227/ https://www.ncbi.nlm.nih.gov/pubmed/35864104 http://dx.doi.org/10.1038/s41467-022-31796-1 |
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