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A potential preventive method for scar stenosis after esophageal endoscopic mucosal resection using human amniotic epithelial cells in a porcine model
OBJECTIVES: The current methods employed for esophageal endoscopic mucosal resection (EMR) involve the risk of adverse postprocedural complications. Therefore, this study aimed to develop a new method to prevent stenosis following a resection procedure using human amniotic epithelial cells in a porc...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302276/ https://www.ncbi.nlm.nih.gov/pubmed/35873524 http://dx.doi.org/10.1002/deo2.104 |
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author | Konno, Yuji Sato, Chiaki Tokodai, Kazuaki Saito, Masatoshi Hoshiai, Tetsuro Taniyama, Yusuke Okamoto, Hiroshi Fukutomi, Toshiaki Ozawa, Yohei Ujiie, Naoto Koseki, Ken Ando, Ryohei Takahashi, Kozue Shinozaki, Yasuharu Unno, Michiaki Kamei, Takashi |
author_facet | Konno, Yuji Sato, Chiaki Tokodai, Kazuaki Saito, Masatoshi Hoshiai, Tetsuro Taniyama, Yusuke Okamoto, Hiroshi Fukutomi, Toshiaki Ozawa, Yohei Ujiie, Naoto Koseki, Ken Ando, Ryohei Takahashi, Kozue Shinozaki, Yasuharu Unno, Michiaki Kamei, Takashi |
author_sort | Konno, Yuji |
collection | PubMed |
description | OBJECTIVES: The current methods employed for esophageal endoscopic mucosal resection (EMR) involve the risk of adverse postprocedural complications. Therefore, this study aimed to develop a new method to prevent stenosis following a resection procedure using human amniotic epithelial cells in a porcine model. METHODS: With the consent of a woman who underwent a cesarean section, amniotic epithelial cells were isolated from the amniotic membrane of the delivered placenta. Six swine were used for this study. Under general anesthesia, four EMRs using cap‐fitted microscope ulcers were performed on each porcine esophagus. Of the four ulcers, the two on the oral side were treated by injecting human amniotic epithelial (AE group) cells, and the remaining two on the anal side were left untreated (control group). One week after the procedure, the swine were sacrificed, and the ulcers were evaluated. The epithelialization rate was calculated by dividing the length of the epithelialized portion of each section by the length of the ulcer, which was determined using an optical microscope. Moreover, the mucosal thickening in each section was measured in terms of diameter. RESULTS: The epithelialization rate was significantly higher in the AE group than in the control group. Mucosal thickening was not significantly different between the groups. CONCLUSIONS: Transplanting amniotic epithelial cells into the ulcer promoted ulcer epithelialization. Amniotic epithelial cell transplantation is a potential method for the management of ulcer scar stenosis following esophageal endoscopic submucosal dissection. |
format | Online Article Text |
id | pubmed-9302276 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93022762022-07-22 A potential preventive method for scar stenosis after esophageal endoscopic mucosal resection using human amniotic epithelial cells in a porcine model Konno, Yuji Sato, Chiaki Tokodai, Kazuaki Saito, Masatoshi Hoshiai, Tetsuro Taniyama, Yusuke Okamoto, Hiroshi Fukutomi, Toshiaki Ozawa, Yohei Ujiie, Naoto Koseki, Ken Ando, Ryohei Takahashi, Kozue Shinozaki, Yasuharu Unno, Michiaki Kamei, Takashi DEN Open Original Articles OBJECTIVES: The current methods employed for esophageal endoscopic mucosal resection (EMR) involve the risk of adverse postprocedural complications. Therefore, this study aimed to develop a new method to prevent stenosis following a resection procedure using human amniotic epithelial cells in a porcine model. METHODS: With the consent of a woman who underwent a cesarean section, amniotic epithelial cells were isolated from the amniotic membrane of the delivered placenta. Six swine were used for this study. Under general anesthesia, four EMRs using cap‐fitted microscope ulcers were performed on each porcine esophagus. Of the four ulcers, the two on the oral side were treated by injecting human amniotic epithelial (AE group) cells, and the remaining two on the anal side were left untreated (control group). One week after the procedure, the swine were sacrificed, and the ulcers were evaluated. The epithelialization rate was calculated by dividing the length of the epithelialized portion of each section by the length of the ulcer, which was determined using an optical microscope. Moreover, the mucosal thickening in each section was measured in terms of diameter. RESULTS: The epithelialization rate was significantly higher in the AE group than in the control group. Mucosal thickening was not significantly different between the groups. CONCLUSIONS: Transplanting amniotic epithelial cells into the ulcer promoted ulcer epithelialization. Amniotic epithelial cell transplantation is a potential method for the management of ulcer scar stenosis following esophageal endoscopic submucosal dissection. John Wiley and Sons Inc. 2022-03-09 /pmc/articles/PMC9302276/ /pubmed/35873524 http://dx.doi.org/10.1002/deo2.104 Text en © 2022 The Authors. DEN Open published by John Wiley & Sons Australia, Ltd on behalf of Japan Gastroenterological Endoscopy Society https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Konno, Yuji Sato, Chiaki Tokodai, Kazuaki Saito, Masatoshi Hoshiai, Tetsuro Taniyama, Yusuke Okamoto, Hiroshi Fukutomi, Toshiaki Ozawa, Yohei Ujiie, Naoto Koseki, Ken Ando, Ryohei Takahashi, Kozue Shinozaki, Yasuharu Unno, Michiaki Kamei, Takashi A potential preventive method for scar stenosis after esophageal endoscopic mucosal resection using human amniotic epithelial cells in a porcine model |
title | A potential preventive method for scar stenosis after esophageal endoscopic mucosal resection using human amniotic epithelial cells in a porcine model |
title_full | A potential preventive method for scar stenosis after esophageal endoscopic mucosal resection using human amniotic epithelial cells in a porcine model |
title_fullStr | A potential preventive method for scar stenosis after esophageal endoscopic mucosal resection using human amniotic epithelial cells in a porcine model |
title_full_unstemmed | A potential preventive method for scar stenosis after esophageal endoscopic mucosal resection using human amniotic epithelial cells in a porcine model |
title_short | A potential preventive method for scar stenosis after esophageal endoscopic mucosal resection using human amniotic epithelial cells in a porcine model |
title_sort | potential preventive method for scar stenosis after esophageal endoscopic mucosal resection using human amniotic epithelial cells in a porcine model |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302276/ https://www.ncbi.nlm.nih.gov/pubmed/35873524 http://dx.doi.org/10.1002/deo2.104 |
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