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A Novel Transcriptional Regulator HbERF6 Regulates the HbCIPK2-Coordinated Pathway Conferring Salt Tolerance in Halophytic Hordeum brevisubulatum

Halophytic Hordeum brevisubulatum is a perennial grass which has evolved many distinctive salt-adaptive mechanisms. Our previous studies indicated it could thrive under salt stress through maintaining better K(+) and Na(+) homeostasis. Stress-responsive HbCIPK2 can phosphorylate K(+) channel HbVGKC1...

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Detalles Bibliográficos
Autores principales: Jiang, Ying, Zhang, Haiwen, Li, Yang, Chang, Congcong, Wang, Yunxiao, Feng, Hao, Li, Ruifen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302439/
https://www.ncbi.nlm.nih.gov/pubmed/35873960
http://dx.doi.org/10.3389/fpls.2022.927253
Descripción
Sumario:Halophytic Hordeum brevisubulatum is a perennial grass which has evolved many distinctive salt-adaptive mechanisms. Our previous studies indicated it could thrive under salt stress through maintaining better K(+) and Na(+) homeostasis. Stress-responsive HbCIPK2 can phosphorylate K(+) channel HbVGKC1 and Na(+) transporter HbSOS1L to prevent Na(+) accumulation and K(+) reduction, hence pathway was not detected in glycophytic plants. In this study, we cloned the inducible promoter of HbCIPK2 by genome-walking, and identified a novel transcriptional regulator HbERF6 through yeast one-hybrid screening. HbERF6 functioned as a transcription factor which can bind to the GCC-box of the HbCIPK2 promoter to activate its expression. HbERF6 transgenic lines in Arabidopsis improved salt tolerance compared with wild type, and especially induced AtCIPK24 (SOS2) expression, resulting in K(+)/Na(+) homeostasis to enhance salt tolerance. All the results confirmed the inducible function of HbERF6 for CIPK genes during salt tolerance. This regulatory network that integrates transcriptional regulation and post-translation modification will unravel a novel salt stress-responsive mechanism, highlighting the value and utilization of the halophytic resource.