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Genome-Wide Analysis of CqCrRLK1L and CqRALF Gene Families in Chenopodium quinoa and Their Roles in Salt Stress Response

Chenopodium quinoa is a halophyte with exceptional nutritional qualities, and therefore it is potentially an ideal crop to grow in saline soils, not only addressing the problem of land salinization, but also providing nutrient food for the health of humans. Currently, the molecular mechanisms underl...

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Autores principales: Jiang, Wei, Li, Chao, Li, Leiting, Li, Yali, Wang, Zhihao, Yu, Feiyu, Yi, Feng, Zhang, Jianhan, Zhu, Jian-Kang, Zhang, Heng, Li, Yan, Zhao, Chunzhao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302450/
https://www.ncbi.nlm.nih.gov/pubmed/35873972
http://dx.doi.org/10.3389/fpls.2022.918594
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author Jiang, Wei
Li, Chao
Li, Leiting
Li, Yali
Wang, Zhihao
Yu, Feiyu
Yi, Feng
Zhang, Jianhan
Zhu, Jian-Kang
Zhang, Heng
Li, Yan
Zhao, Chunzhao
author_facet Jiang, Wei
Li, Chao
Li, Leiting
Li, Yali
Wang, Zhihao
Yu, Feiyu
Yi, Feng
Zhang, Jianhan
Zhu, Jian-Kang
Zhang, Heng
Li, Yan
Zhao, Chunzhao
author_sort Jiang, Wei
collection PubMed
description Chenopodium quinoa is a halophyte with exceptional nutritional qualities, and therefore it is potentially an ideal crop to grow in saline soils, not only addressing the problem of land salinization, but also providing nutrient food for the health of humans. Currently, the molecular mechanisms underlying salt tolerance in quinoa are still largely unknown. In Arabidopsis thaliana, Catharanthus roseus receptor-like kinase (CrRLK1Ls) FERONIA (FER) and its ligands rapid alkalinization factors (RALFs) have been reported that participate in the regulation of salt tolerance. Here, we performed a genome-wide analysis and identified 26 CqCrRLK1L and 18 CqRALF family genes in quinoa genome. Transcriptomic profiling of the leaf, root, stamen, and pistil tissues of quinoa reveals that different CqCrRLK1L and CqRALF genes exhibit tissue-specific expression patterns, which is consistent with that observed in other plant species. RNA-seq data show that three CqCrRLK1L genes are highly up-regulated after salt treatment, suggesting that some CqCrRLK1L family genes are transcriptionally responsive to salt stress in quinoa. Biochemical study indicates that CqRALF15, a paralog of Arabidopsis RALF22, is physically associated with CrRLK1L proteins CqFER and AtFER. CqRALF15 and AtRALF22 are functionally conserved in inducing the internalization of AtFER and in triggering root growth inhibition in both quinoa and Arabidopsis. Moreover, overexpression of CqRALF15 in Arabidopsis results in enhanced leaf bleaching under salt stress, indicating that CqRALF15 is involved in salt stress response. Together, our study characterizes CqCrRLK1L and CqRALF family genes in quinoa at genomic, transcriptional, and protein levels, and provides evidence to support their roles in salt stress response.
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spelling pubmed-93024502022-07-22 Genome-Wide Analysis of CqCrRLK1L and CqRALF Gene Families in Chenopodium quinoa and Their Roles in Salt Stress Response Jiang, Wei Li, Chao Li, Leiting Li, Yali Wang, Zhihao Yu, Feiyu Yi, Feng Zhang, Jianhan Zhu, Jian-Kang Zhang, Heng Li, Yan Zhao, Chunzhao Front Plant Sci Plant Science Chenopodium quinoa is a halophyte with exceptional nutritional qualities, and therefore it is potentially an ideal crop to grow in saline soils, not only addressing the problem of land salinization, but also providing nutrient food for the health of humans. Currently, the molecular mechanisms underlying salt tolerance in quinoa are still largely unknown. In Arabidopsis thaliana, Catharanthus roseus receptor-like kinase (CrRLK1Ls) FERONIA (FER) and its ligands rapid alkalinization factors (RALFs) have been reported that participate in the regulation of salt tolerance. Here, we performed a genome-wide analysis and identified 26 CqCrRLK1L and 18 CqRALF family genes in quinoa genome. Transcriptomic profiling of the leaf, root, stamen, and pistil tissues of quinoa reveals that different CqCrRLK1L and CqRALF genes exhibit tissue-specific expression patterns, which is consistent with that observed in other plant species. RNA-seq data show that three CqCrRLK1L genes are highly up-regulated after salt treatment, suggesting that some CqCrRLK1L family genes are transcriptionally responsive to salt stress in quinoa. Biochemical study indicates that CqRALF15, a paralog of Arabidopsis RALF22, is physically associated with CrRLK1L proteins CqFER and AtFER. CqRALF15 and AtRALF22 are functionally conserved in inducing the internalization of AtFER and in triggering root growth inhibition in both quinoa and Arabidopsis. Moreover, overexpression of CqRALF15 in Arabidopsis results in enhanced leaf bleaching under salt stress, indicating that CqRALF15 is involved in salt stress response. Together, our study characterizes CqCrRLK1L and CqRALF family genes in quinoa at genomic, transcriptional, and protein levels, and provides evidence to support their roles in salt stress response. Frontiers Media S.A. 2022-07-07 /pmc/articles/PMC9302450/ /pubmed/35873972 http://dx.doi.org/10.3389/fpls.2022.918594 Text en Copyright © 2022 Jiang, Li, Li, Li, Wang, Yu, Yi, Zhang, Zhu, Zhang, Li and Zhao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Jiang, Wei
Li, Chao
Li, Leiting
Li, Yali
Wang, Zhihao
Yu, Feiyu
Yi, Feng
Zhang, Jianhan
Zhu, Jian-Kang
Zhang, Heng
Li, Yan
Zhao, Chunzhao
Genome-Wide Analysis of CqCrRLK1L and CqRALF Gene Families in Chenopodium quinoa and Their Roles in Salt Stress Response
title Genome-Wide Analysis of CqCrRLK1L and CqRALF Gene Families in Chenopodium quinoa and Their Roles in Salt Stress Response
title_full Genome-Wide Analysis of CqCrRLK1L and CqRALF Gene Families in Chenopodium quinoa and Their Roles in Salt Stress Response
title_fullStr Genome-Wide Analysis of CqCrRLK1L and CqRALF Gene Families in Chenopodium quinoa and Their Roles in Salt Stress Response
title_full_unstemmed Genome-Wide Analysis of CqCrRLK1L and CqRALF Gene Families in Chenopodium quinoa and Their Roles in Salt Stress Response
title_short Genome-Wide Analysis of CqCrRLK1L and CqRALF Gene Families in Chenopodium quinoa and Their Roles in Salt Stress Response
title_sort genome-wide analysis of cqcrrlk1l and cqralf gene families in chenopodium quinoa and their roles in salt stress response
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302450/
https://www.ncbi.nlm.nih.gov/pubmed/35873972
http://dx.doi.org/10.3389/fpls.2022.918594
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