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FLA11 and FLA12 glycoproteins fine‐tune stem secondary wall properties in response to mechanical stresses
Secondary cell walls (SCWs) in stem xylem vessel and fibre cells enable plants to withstand the enormous compressive forces associated with upright growth. It remains unclear if xylem vessel and fibre cells can directly sense mechanical stimuli and modify their SCW during development. We provide evi...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302641/ https://www.ncbi.nlm.nih.gov/pubmed/34862967 http://dx.doi.org/10.1111/nph.17898 |
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author | Ma, Yingxuan MacMillan, Colleen P. de Vries, Lisanne Mansfield, Shawn D. Hao, Pengfei Ratcliffe, Julian Bacic, Antony Johnson, Kim L. |
author_facet | Ma, Yingxuan MacMillan, Colleen P. de Vries, Lisanne Mansfield, Shawn D. Hao, Pengfei Ratcliffe, Julian Bacic, Antony Johnson, Kim L. |
author_sort | Ma, Yingxuan |
collection | PubMed |
description | Secondary cell walls (SCWs) in stem xylem vessel and fibre cells enable plants to withstand the enormous compressive forces associated with upright growth. It remains unclear if xylem vessel and fibre cells can directly sense mechanical stimuli and modify their SCW during development. We provide evidence that Arabidopsis SCW‐specific Fasciclin‐Like Arabinogalactan‐proteins 11 (FLA11) and 12 (FLA12) are possible cell surface sensors regulating SCW development in response to mechanical stimuli. Plants overexpressing FLA11 (OE‐FLA11) showed earlier SCW development compared to the wild‐type (WT) and altered SCW properties that phenocopy WT plants under compression stress. By contrast, OE‐FLA12 stems showed higher cellulose content compared to WT plants, similar to plants experiencing tensile stress. fla11, OE‐FLA11, fla12, and OE‐FLA12 plants showed altered SCW responses to mechanical stress compared to the WT. Quantitative polymerase chain reaction (qPCR) and RNA‐seq analysis revealed the up‐regulation of genes and pathways involved in stress responses and SCW synthesis and regulation. Analysis of OE‐FLA11 nst1 nst3 plants suggests that FLA11 regulation of SCWs is reliant on classical transcriptional networks. Our data support the involvement of FLA11 and FLA12 in SCW sensing complexes to fine‐tune both the initiation of SCW development and the balance of lignin and cellulose synthesis/deposition in SCWs during development and in response to mechanical stimuli. |
format | Online Article Text |
id | pubmed-9302641 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93026412022-07-22 FLA11 and FLA12 glycoproteins fine‐tune stem secondary wall properties in response to mechanical stresses Ma, Yingxuan MacMillan, Colleen P. de Vries, Lisanne Mansfield, Shawn D. Hao, Pengfei Ratcliffe, Julian Bacic, Antony Johnson, Kim L. New Phytol Research Secondary cell walls (SCWs) in stem xylem vessel and fibre cells enable plants to withstand the enormous compressive forces associated with upright growth. It remains unclear if xylem vessel and fibre cells can directly sense mechanical stimuli and modify their SCW during development. We provide evidence that Arabidopsis SCW‐specific Fasciclin‐Like Arabinogalactan‐proteins 11 (FLA11) and 12 (FLA12) are possible cell surface sensors regulating SCW development in response to mechanical stimuli. Plants overexpressing FLA11 (OE‐FLA11) showed earlier SCW development compared to the wild‐type (WT) and altered SCW properties that phenocopy WT plants under compression stress. By contrast, OE‐FLA12 stems showed higher cellulose content compared to WT plants, similar to plants experiencing tensile stress. fla11, OE‐FLA11, fla12, and OE‐FLA12 plants showed altered SCW responses to mechanical stress compared to the WT. Quantitative polymerase chain reaction (qPCR) and RNA‐seq analysis revealed the up‐regulation of genes and pathways involved in stress responses and SCW synthesis and regulation. Analysis of OE‐FLA11 nst1 nst3 plants suggests that FLA11 regulation of SCWs is reliant on classical transcriptional networks. Our data support the involvement of FLA11 and FLA12 in SCW sensing complexes to fine‐tune both the initiation of SCW development and the balance of lignin and cellulose synthesis/deposition in SCWs during development and in response to mechanical stimuli. John Wiley and Sons Inc. 2022-01-04 2022-02 /pmc/articles/PMC9302641/ /pubmed/34862967 http://dx.doi.org/10.1111/nph.17898 Text en © 2021 The Authors. New Phytologist © 2021 New Phytologist Foundation https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Ma, Yingxuan MacMillan, Colleen P. de Vries, Lisanne Mansfield, Shawn D. Hao, Pengfei Ratcliffe, Julian Bacic, Antony Johnson, Kim L. FLA11 and FLA12 glycoproteins fine‐tune stem secondary wall properties in response to mechanical stresses |
title | FLA11 and FLA12 glycoproteins fine‐tune stem secondary wall properties in response to mechanical stresses |
title_full | FLA11 and FLA12 glycoproteins fine‐tune stem secondary wall properties in response to mechanical stresses |
title_fullStr | FLA11 and FLA12 glycoproteins fine‐tune stem secondary wall properties in response to mechanical stresses |
title_full_unstemmed | FLA11 and FLA12 glycoproteins fine‐tune stem secondary wall properties in response to mechanical stresses |
title_short | FLA11 and FLA12 glycoproteins fine‐tune stem secondary wall properties in response to mechanical stresses |
title_sort | fla11 and fla12 glycoproteins fine‐tune stem secondary wall properties in response to mechanical stresses |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302641/ https://www.ncbi.nlm.nih.gov/pubmed/34862967 http://dx.doi.org/10.1111/nph.17898 |
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