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The structural role of SARS-CoV-2 genetic background in the emergence and success of spike mutations: The case of the spike A222V mutation
The S:A222V point mutation, within the G clade, was characteristic of the 20E (EU1) SARS-CoV-2 variant identified in Spain in early summer 2020. This mutation has since reappeared in the Delta subvariant AY.4.2, raising questions about its specific effect on viral infection. We report combined serol...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302720/ https://www.ncbi.nlm.nih.gov/pubmed/35816514 http://dx.doi.org/10.1371/journal.ppat.1010631 |
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| author | Ginex, Tiziana Marco-Marín, Clara Wieczór, Miłosz Mata, Carlos P. Krieger, James Ruiz-Rodriguez, Paula López-Redondo, Maria Luisa Francés-Gómez, Clara Melero, Roberto Sánchez-Sorzano, Carlos Óscar Martínez, Marta Gougeard, Nadine Forcada-Nadal, Alicia Zamora-Caballero, Sara Gozalbo-Rovira, Roberto Sanz-Frasquet, Carla Arranz, Rocío Bravo, Jeronimo Rubio, Vicente Marina, Alberto Geller, Ron Comas, Iñaki Gil, Carmen Coscolla, Mireia Orozco, Modesto Llácer, José Luis Carazo, Jose-Maria |
| author_facet | Ginex, Tiziana Marco-Marín, Clara Wieczór, Miłosz Mata, Carlos P. Krieger, James Ruiz-Rodriguez, Paula López-Redondo, Maria Luisa Francés-Gómez, Clara Melero, Roberto Sánchez-Sorzano, Carlos Óscar Martínez, Marta Gougeard, Nadine Forcada-Nadal, Alicia Zamora-Caballero, Sara Gozalbo-Rovira, Roberto Sanz-Frasquet, Carla Arranz, Rocío Bravo, Jeronimo Rubio, Vicente Marina, Alberto Geller, Ron Comas, Iñaki Gil, Carmen Coscolla, Mireia Orozco, Modesto Llácer, José Luis Carazo, Jose-Maria |
| author_sort | Ginex, Tiziana |
| collection | PubMed |
| description | The S:A222V point mutation, within the G clade, was characteristic of the 20E (EU1) SARS-CoV-2 variant identified in Spain in early summer 2020. This mutation has since reappeared in the Delta subvariant AY.4.2, raising questions about its specific effect on viral infection. We report combined serological, functional, structural and computational studies characterizing the impact of this mutation. Our results reveal that S:A222V promotes an increased RBD opening and slightly increases ACE2 binding as compared to the parent S:D614G clade. Finally, S:A222V does not reduce sera neutralization capacity, suggesting it does not affect vaccine effectiveness. |
| format | Online Article Text |
| id | pubmed-9302720 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2022 |
| publisher | Public Library of Science |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-93027202022-07-22 The structural role of SARS-CoV-2 genetic background in the emergence and success of spike mutations: The case of the spike A222V mutation Ginex, Tiziana Marco-Marín, Clara Wieczór, Miłosz Mata, Carlos P. Krieger, James Ruiz-Rodriguez, Paula López-Redondo, Maria Luisa Francés-Gómez, Clara Melero, Roberto Sánchez-Sorzano, Carlos Óscar Martínez, Marta Gougeard, Nadine Forcada-Nadal, Alicia Zamora-Caballero, Sara Gozalbo-Rovira, Roberto Sanz-Frasquet, Carla Arranz, Rocío Bravo, Jeronimo Rubio, Vicente Marina, Alberto Geller, Ron Comas, Iñaki Gil, Carmen Coscolla, Mireia Orozco, Modesto Llácer, José Luis Carazo, Jose-Maria PLoS Pathog Research Article The S:A222V point mutation, within the G clade, was characteristic of the 20E (EU1) SARS-CoV-2 variant identified in Spain in early summer 2020. This mutation has since reappeared in the Delta subvariant AY.4.2, raising questions about its specific effect on viral infection. We report combined serological, functional, structural and computational studies characterizing the impact of this mutation. Our results reveal that S:A222V promotes an increased RBD opening and slightly increases ACE2 binding as compared to the parent S:D614G clade. Finally, S:A222V does not reduce sera neutralization capacity, suggesting it does not affect vaccine effectiveness. Public Library of Science 2022-07-11 /pmc/articles/PMC9302720/ /pubmed/35816514 http://dx.doi.org/10.1371/journal.ppat.1010631 Text en © 2022 Ginex et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| spellingShingle | Research Article Ginex, Tiziana Marco-Marín, Clara Wieczór, Miłosz Mata, Carlos P. Krieger, James Ruiz-Rodriguez, Paula López-Redondo, Maria Luisa Francés-Gómez, Clara Melero, Roberto Sánchez-Sorzano, Carlos Óscar Martínez, Marta Gougeard, Nadine Forcada-Nadal, Alicia Zamora-Caballero, Sara Gozalbo-Rovira, Roberto Sanz-Frasquet, Carla Arranz, Rocío Bravo, Jeronimo Rubio, Vicente Marina, Alberto Geller, Ron Comas, Iñaki Gil, Carmen Coscolla, Mireia Orozco, Modesto Llácer, José Luis Carazo, Jose-Maria The structural role of SARS-CoV-2 genetic background in the emergence and success of spike mutations: The case of the spike A222V mutation |
| title | The structural role of SARS-CoV-2 genetic background in the emergence and success of spike mutations: The case of the spike A222V mutation |
| title_full | The structural role of SARS-CoV-2 genetic background in the emergence and success of spike mutations: The case of the spike A222V mutation |
| title_fullStr | The structural role of SARS-CoV-2 genetic background in the emergence and success of spike mutations: The case of the spike A222V mutation |
| title_full_unstemmed | The structural role of SARS-CoV-2 genetic background in the emergence and success of spike mutations: The case of the spike A222V mutation |
| title_short | The structural role of SARS-CoV-2 genetic background in the emergence and success of spike mutations: The case of the spike A222V mutation |
| title_sort | structural role of sars-cov-2 genetic background in the emergence and success of spike mutations: the case of the spike a222v mutation |
| topic | Research Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302720/ https://www.ncbi.nlm.nih.gov/pubmed/35816514 http://dx.doi.org/10.1371/journal.ppat.1010631 |
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