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Estimating the genetic structure of Triatoma dimidiata (Hemiptera: Reduviidae) and the transmission dynamics of Trypanosoma cruzi in Boyacá, eastern Colombia
Chagas disease is considered a public health issue in Colombia, where many regions are endemic. Triatoma dimidiata is an important vector after Rhodnius prolixus, and it is gaining importance in Boyacá, eastern Colombia. Following the recent elimination of R. prolixus in the region, it is pivotal to...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302734/ https://www.ncbi.nlm.nih.gov/pubmed/35816541 http://dx.doi.org/10.1371/journal.pntd.0010534 |
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author | Velásquez-Ortiz, Natalia Hernández, Carolina Cantillo-Barraza, Omar Medina, Manuel Medina-Alfonso, Mabel Suescún-Carrero, Sandra Muñoz, Marina Vega, Laura Castañeda, Sergio Cruz-Saavedra, Lissa Ballesteros, Nathalia Ramírez, Juan David |
author_facet | Velásquez-Ortiz, Natalia Hernández, Carolina Cantillo-Barraza, Omar Medina, Manuel Medina-Alfonso, Mabel Suescún-Carrero, Sandra Muñoz, Marina Vega, Laura Castañeda, Sergio Cruz-Saavedra, Lissa Ballesteros, Nathalia Ramírez, Juan David |
author_sort | Velásquez-Ortiz, Natalia |
collection | PubMed |
description | Chagas disease is considered a public health issue in Colombia, where many regions are endemic. Triatoma dimidiata is an important vector after Rhodnius prolixus, and it is gaining importance in Boyacá, eastern Colombia. Following the recent elimination of R. prolixus in the region, it is pivotal to understand the behavior of T. dimidiata and the transmission dynamics of T. cruzi. We used qPCR and Next Generation Sequencing (NGS) to evaluate T. cruzi infection, parasite load, feeding profiles, and T. cruzi genotyping for T. dimidiata specimens collected in nine municipalities in Boyacá and explored T. dimidiata population genetics. We found that T. dimidiata populations are composed by a single population with similar genetic characteristics that present infection rates up to 70%, high parasite loads up to 1.46 × 10(9) parasite-equivalents/mL, a feeding behavior that comprises at least 17 domestic, synanthropic and sylvatic species, and a wide diversity of TcI genotypes even within a single specimen. These results imply that T. dimidiata behavior is similar to other successful vectors, having a wide variety of blood sources and contributing to the circulation of different genotypes of the parasite, highlighting its importance for T. cruzi transmission and risk for humans. In the light of the elimination of R. prolixus in Boyacá and the results we found, we suggest that T. dimidiata should become a new target for vector control programs. We hope this study provides enough information to enhance surveillance programs and a future effective interruption of T. cruzi vector transmission in endemic regions. |
format | Online Article Text |
id | pubmed-9302734 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-93027342022-07-22 Estimating the genetic structure of Triatoma dimidiata (Hemiptera: Reduviidae) and the transmission dynamics of Trypanosoma cruzi in Boyacá, eastern Colombia Velásquez-Ortiz, Natalia Hernández, Carolina Cantillo-Barraza, Omar Medina, Manuel Medina-Alfonso, Mabel Suescún-Carrero, Sandra Muñoz, Marina Vega, Laura Castañeda, Sergio Cruz-Saavedra, Lissa Ballesteros, Nathalia Ramírez, Juan David PLoS Negl Trop Dis Research Article Chagas disease is considered a public health issue in Colombia, where many regions are endemic. Triatoma dimidiata is an important vector after Rhodnius prolixus, and it is gaining importance in Boyacá, eastern Colombia. Following the recent elimination of R. prolixus in the region, it is pivotal to understand the behavior of T. dimidiata and the transmission dynamics of T. cruzi. We used qPCR and Next Generation Sequencing (NGS) to evaluate T. cruzi infection, parasite load, feeding profiles, and T. cruzi genotyping for T. dimidiata specimens collected in nine municipalities in Boyacá and explored T. dimidiata population genetics. We found that T. dimidiata populations are composed by a single population with similar genetic characteristics that present infection rates up to 70%, high parasite loads up to 1.46 × 10(9) parasite-equivalents/mL, a feeding behavior that comprises at least 17 domestic, synanthropic and sylvatic species, and a wide diversity of TcI genotypes even within a single specimen. These results imply that T. dimidiata behavior is similar to other successful vectors, having a wide variety of blood sources and contributing to the circulation of different genotypes of the parasite, highlighting its importance for T. cruzi transmission and risk for humans. In the light of the elimination of R. prolixus in Boyacá and the results we found, we suggest that T. dimidiata should become a new target for vector control programs. We hope this study provides enough information to enhance surveillance programs and a future effective interruption of T. cruzi vector transmission in endemic regions. Public Library of Science 2022-07-11 /pmc/articles/PMC9302734/ /pubmed/35816541 http://dx.doi.org/10.1371/journal.pntd.0010534 Text en © 2022 Velásquez-Ortiz et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Velásquez-Ortiz, Natalia Hernández, Carolina Cantillo-Barraza, Omar Medina, Manuel Medina-Alfonso, Mabel Suescún-Carrero, Sandra Muñoz, Marina Vega, Laura Castañeda, Sergio Cruz-Saavedra, Lissa Ballesteros, Nathalia Ramírez, Juan David Estimating the genetic structure of Triatoma dimidiata (Hemiptera: Reduviidae) and the transmission dynamics of Trypanosoma cruzi in Boyacá, eastern Colombia |
title | Estimating the genetic structure of Triatoma dimidiata (Hemiptera: Reduviidae) and the transmission dynamics of Trypanosoma cruzi in Boyacá, eastern Colombia |
title_full | Estimating the genetic structure of Triatoma dimidiata (Hemiptera: Reduviidae) and the transmission dynamics of Trypanosoma cruzi in Boyacá, eastern Colombia |
title_fullStr | Estimating the genetic structure of Triatoma dimidiata (Hemiptera: Reduviidae) and the transmission dynamics of Trypanosoma cruzi in Boyacá, eastern Colombia |
title_full_unstemmed | Estimating the genetic structure of Triatoma dimidiata (Hemiptera: Reduviidae) and the transmission dynamics of Trypanosoma cruzi in Boyacá, eastern Colombia |
title_short | Estimating the genetic structure of Triatoma dimidiata (Hemiptera: Reduviidae) and the transmission dynamics of Trypanosoma cruzi in Boyacá, eastern Colombia |
title_sort | estimating the genetic structure of triatoma dimidiata (hemiptera: reduviidae) and the transmission dynamics of trypanosoma cruzi in boyacá, eastern colombia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302734/ https://www.ncbi.nlm.nih.gov/pubmed/35816541 http://dx.doi.org/10.1371/journal.pntd.0010534 |
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