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A feasible and practical (1)H NMR analytical method for the quality control and quantification of bioactive principles in Lycii Fructus

Lycii Fructus, a solanaceous drug, is widely used as functional foods and in Traditional Chinese Medicine. Samples collected from different regions of China have been found to be not identical in chemical compositions which might affect the biological activities. Although many chromatographic and sp...

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Detalles Bibliográficos
Autores principales: Hsieh, Li-Ying, Chan, Hsiu-Hui, Kuo, Ping-Chung, Hung, Hsin-Yi, Li, Yue-Chiun, Kuo, Chao-Lin, Peng, Yong, Zhao, Zhong-Zhen, Kuo, Daih-Huang, Sun, I-Wen, Wu, Tian-Shung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taiwan Food and Drug Administration 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303018/
https://www.ncbi.nlm.nih.gov/pubmed/29976403
http://dx.doi.org/10.1016/j.jfda.2018.01.001
Descripción
Sumario:Lycii Fructus, a solanaceous drug, is widely used as functional foods and in Traditional Chinese Medicine. Samples collected from different regions of China have been found to be not identical in chemical compositions which might affect the biological activities. Although many chromatographic and spectrometric methods have been reported to determine the concentration of betaine and other bioactive amino acids, disturbance resulted from other polar substances with low UV-absorbance and expensive mass facilities reduced the applicability of these techniques. In the present study, the strong cation exchange solid phase extraction procedure incorporated with (1)H NMR was successfully developed as a rapid and reliable method that can simultaneously determine betaine, citric acid, threonine, alanine, and proline in various Lycii Fructus. In addition, ERETIC 2 method based on PULCON principle was also applied and compared with conventional method. This feasible and practical method offers a very powerful tool for the quality control of commercial Lycii Fructus from different sources.