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Organohalide respiration potential in marine sediments from Aarhus Bay

Organohalide respiration (OHR), catalysed by reductive dehalogenases (RDases), plays an important role in halogen cycling. Natural organohalides and putative RDase-encoding genes have been reported in Aarhus Bay sediments, however, OHR has not been experimentally verified. Here we show that sediment...

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Autores principales: Zhang, Chen, Atashgahi, Siavash, Bosma, Tom N P, Peng, Peng, Smidt, Hauke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303371/
https://www.ncbi.nlm.nih.gov/pubmed/35689665
http://dx.doi.org/10.1093/femsec/fiac073
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author Zhang, Chen
Atashgahi, Siavash
Bosma, Tom N P
Peng, Peng
Smidt, Hauke
author_facet Zhang, Chen
Atashgahi, Siavash
Bosma, Tom N P
Peng, Peng
Smidt, Hauke
author_sort Zhang, Chen
collection PubMed
description Organohalide respiration (OHR), catalysed by reductive dehalogenases (RDases), plays an important role in halogen cycling. Natural organohalides and putative RDase-encoding genes have been reported in Aarhus Bay sediments, however, OHR has not been experimentally verified. Here we show that sediments of Aarhus Bay can dehalogenate a range of organohalides, and different organohalides differentially affected microbial community compositions. PCE-dechlorinating cultures were further examined by 16S rRNA gene-targeted quantitative PCR and amplicon sequencing. Known organohalide-respiring bacteria (OHRB) including Dehalococcoides, Dehalobacter and Desulfitobacterium decreased in abundance during transfers and serial dilutions, suggesting the importance of yet uncharacterized OHRB in these cultures. Switching from PCE to 2,6-DBP led to its complete debromination to phenol in cultures with and without sulfate. 2,6-DBP debrominating cultures differed in microbial composition from PCE-dechlorinating cultures. Desulfobacterota genera recently verified to include OHRB, including Desulfovibrio and Desulfuromusa, were enriched in all microcosms, whereas Halodesulfovibrio was only enriched in cultures without sulfate. Hydrogen and methane were detected in cultures without sulfate. Hydrogen likely served as electron donor for OHR and methanogenesis. This study shows that OHR can occur in marine environments mediated by yet unknown OHRB, suggesting their role in natural halogen cycling.
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spelling pubmed-93033712022-07-22 Organohalide respiration potential in marine sediments from Aarhus Bay Zhang, Chen Atashgahi, Siavash Bosma, Tom N P Peng, Peng Smidt, Hauke FEMS Microbiol Ecol Research Article Organohalide respiration (OHR), catalysed by reductive dehalogenases (RDases), plays an important role in halogen cycling. Natural organohalides and putative RDase-encoding genes have been reported in Aarhus Bay sediments, however, OHR has not been experimentally verified. Here we show that sediments of Aarhus Bay can dehalogenate a range of organohalides, and different organohalides differentially affected microbial community compositions. PCE-dechlorinating cultures were further examined by 16S rRNA gene-targeted quantitative PCR and amplicon sequencing. Known organohalide-respiring bacteria (OHRB) including Dehalococcoides, Dehalobacter and Desulfitobacterium decreased in abundance during transfers and serial dilutions, suggesting the importance of yet uncharacterized OHRB in these cultures. Switching from PCE to 2,6-DBP led to its complete debromination to phenol in cultures with and without sulfate. 2,6-DBP debrominating cultures differed in microbial composition from PCE-dechlorinating cultures. Desulfobacterota genera recently verified to include OHRB, including Desulfovibrio and Desulfuromusa, were enriched in all microcosms, whereas Halodesulfovibrio was only enriched in cultures without sulfate. Hydrogen and methane were detected in cultures without sulfate. Hydrogen likely served as electron donor for OHR and methanogenesis. This study shows that OHR can occur in marine environments mediated by yet unknown OHRB, suggesting their role in natural halogen cycling. Oxford University Press 2022-06-11 /pmc/articles/PMC9303371/ /pubmed/35689665 http://dx.doi.org/10.1093/femsec/fiac073 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of FEMS. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Chen
Atashgahi, Siavash
Bosma, Tom N P
Peng, Peng
Smidt, Hauke
Organohalide respiration potential in marine sediments from Aarhus Bay
title Organohalide respiration potential in marine sediments from Aarhus Bay
title_full Organohalide respiration potential in marine sediments from Aarhus Bay
title_fullStr Organohalide respiration potential in marine sediments from Aarhus Bay
title_full_unstemmed Organohalide respiration potential in marine sediments from Aarhus Bay
title_short Organohalide respiration potential in marine sediments from Aarhus Bay
title_sort organohalide respiration potential in marine sediments from aarhus bay
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303371/
https://www.ncbi.nlm.nih.gov/pubmed/35689665
http://dx.doi.org/10.1093/femsec/fiac073
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