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Efficient golden gate assembly of DNA constructs for single molecule force spectroscopy and imaging

Single-molecule techniques such as optical tweezers and fluorescence imaging are powerful tools for probing the biophysics of DNA and DNA-protein interactions. The application of these methods requires efficient approaches for creating designed DNA structures with labels for binding to a surface or...

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Detalles Bibliográficos
Autores principales:	Bell, Nicholas A W, Molloy, Justin E
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2022
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303394/ https://www.ncbi.nlm.nih.gov/pubmed/35489063 http://dx.doi.org/10.1093/nar/gkac300

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