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A teratocyte‐specific serpin from the endoparasitoid wasp Cotesia vestalis inhibits the prophenoloxidase‐activating system of its host Plutella xylostella
Many endoparasitoids adopt several parasitic factors, such as venom, polydnavirus and teratocytes, to suppress the immune response of their associated hosts including melanization for successful parasitism. A teratocyte‐specific expressed serpin gene, designated as CvT‐serpin6, was identified from t...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Ltd.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303735/ https://www.ncbi.nlm.nih.gov/pubmed/34897868 http://dx.doi.org/10.1111/imb.12751 |
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author | Gu, Qijuan Wu, Zhiwei Zhou, Yuenan Wang, Zhizhi Shi, Min Huang, Jianhua Chen, Xuexin |
author_facet | Gu, Qijuan Wu, Zhiwei Zhou, Yuenan Wang, Zhizhi Shi, Min Huang, Jianhua Chen, Xuexin |
author_sort | Gu, Qijuan |
collection | PubMed |
description | Many endoparasitoids adopt several parasitic factors, such as venom, polydnavirus and teratocytes, to suppress the immune response of their associated hosts including melanization for successful parasitism. A teratocyte‐specific expressed serpin gene, designated as CvT‐serpin6, was identified from the parasitoid Cotesia vestalis. The immunoblot result suggested that CvT‐serpin6 was secreted into extracellular space. qPCR results showed that CvT‐serpin6 was mainly transcribed at later stages of parasitism, and the transcriptional abundance of CvT‐serpin6 in teratocytes was significantly increased in response to the challenge of bacteria. Inhibitory assay indicated that recombinant CvT‐serpin6 (rCvT‐serpin6) could inhibit the activation of Plutella xylostella prophenoloxidase and ultimately resulted in the inhibition of melanization in P. xylostella haemolymph. Furthermore, we confirmed that rCvT‐serpin6 could form SDS‐stable complexes with activated PxPAP1 and PxPAP3 in a dose‐dependent manner. Altogether, our results further shed insight into the molecular mechanisms that teratocytes involved in controlling host immune response. |
format | Online Article Text |
id | pubmed-9303735 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley & Sons, Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93037352022-07-28 A teratocyte‐specific serpin from the endoparasitoid wasp Cotesia vestalis inhibits the prophenoloxidase‐activating system of its host Plutella xylostella Gu, Qijuan Wu, Zhiwei Zhou, Yuenan Wang, Zhizhi Shi, Min Huang, Jianhua Chen, Xuexin Insect Mol Biol Original Articles Many endoparasitoids adopt several parasitic factors, such as venom, polydnavirus and teratocytes, to suppress the immune response of their associated hosts including melanization for successful parasitism. A teratocyte‐specific expressed serpin gene, designated as CvT‐serpin6, was identified from the parasitoid Cotesia vestalis. The immunoblot result suggested that CvT‐serpin6 was secreted into extracellular space. qPCR results showed that CvT‐serpin6 was mainly transcribed at later stages of parasitism, and the transcriptional abundance of CvT‐serpin6 in teratocytes was significantly increased in response to the challenge of bacteria. Inhibitory assay indicated that recombinant CvT‐serpin6 (rCvT‐serpin6) could inhibit the activation of Plutella xylostella prophenoloxidase and ultimately resulted in the inhibition of melanization in P. xylostella haemolymph. Furthermore, we confirmed that rCvT‐serpin6 could form SDS‐stable complexes with activated PxPAP1 and PxPAP3 in a dose‐dependent manner. Altogether, our results further shed insight into the molecular mechanisms that teratocytes involved in controlling host immune response. John Wiley & Sons, Ltd. 2021-12-28 2022-04 /pmc/articles/PMC9303735/ /pubmed/34897868 http://dx.doi.org/10.1111/imb.12751 Text en © 2021 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Gu, Qijuan Wu, Zhiwei Zhou, Yuenan Wang, Zhizhi Shi, Min Huang, Jianhua Chen, Xuexin A teratocyte‐specific serpin from the endoparasitoid wasp Cotesia vestalis inhibits the prophenoloxidase‐activating system of its host Plutella xylostella |
title | A teratocyte‐specific serpin from the endoparasitoid wasp Cotesia vestalis inhibits the prophenoloxidase‐activating system of its host Plutella xylostella
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title_full | A teratocyte‐specific serpin from the endoparasitoid wasp Cotesia vestalis inhibits the prophenoloxidase‐activating system of its host Plutella xylostella
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title_fullStr | A teratocyte‐specific serpin from the endoparasitoid wasp Cotesia vestalis inhibits the prophenoloxidase‐activating system of its host Plutella xylostella
|
title_full_unstemmed | A teratocyte‐specific serpin from the endoparasitoid wasp Cotesia vestalis inhibits the prophenoloxidase‐activating system of its host Plutella xylostella
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title_short | A teratocyte‐specific serpin from the endoparasitoid wasp Cotesia vestalis inhibits the prophenoloxidase‐activating system of its host Plutella xylostella
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title_sort | teratocyte‐specific serpin from the endoparasitoid wasp cotesia vestalis inhibits the prophenoloxidase‐activating system of its host plutella xylostella |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303735/ https://www.ncbi.nlm.nih.gov/pubmed/34897868 http://dx.doi.org/10.1111/imb.12751 |
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