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Quantification of the NA dependent change of shape in the image formation of a z‐polarized fluorescent molecule using vectorial diffraction simulations
The point spread function of a fixed fluorophore with its dipole axis colinear to the optical axis appears donut‐shaped when seen through a microscope, and its light distribution in the pupil plane is radially polarized. Yet other techniques, such as photolithography, report that this same light dis...
Autores principales: | Ströhl, Florian, Bruggeman, Ezra, Rowlands, Christopher J., Wolfson, Deanna L., Ahluwalia, Balpreet S. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304265/ https://www.ncbi.nlm.nih.gov/pubmed/35045219 http://dx.doi.org/10.1002/jemt.24060 |
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