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The role of miR-4469 as a tumor suppressor regulating inflammatory cell infiltration in colorectal cancer

BACKGROUND: MicroRNA (miRNA) regulates gene expression posttranscriptionally, and some of them function in tumor suppression and can be used in drug development. As a result, identifying and screening miRNAs that suppress tumors would be a significant addition to tumor treatment. METHODS: In this st...

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Autores principales: Qi, Lu, Wang, Lu, Song, Fuyao, Ding, Zhenhua, Zhang, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research Network of Computational and Structural Biotechnology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304430/
https://www.ncbi.nlm.nih.gov/pubmed/35891783
http://dx.doi.org/10.1016/j.csbj.2022.07.021
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author Qi, Lu
Wang, Lu
Song, Fuyao
Ding, Zhenhua
Zhang, Ying
author_facet Qi, Lu
Wang, Lu
Song, Fuyao
Ding, Zhenhua
Zhang, Ying
author_sort Qi, Lu
collection PubMed
description BACKGROUND: MicroRNA (miRNA) regulates gene expression posttranscriptionally, and some of them function in tumor suppression and can be used in drug development. As a result, identifying and screening miRNAs that suppress tumors would be a significant addition to tumor treatment. METHODS: In this study, we analyzed the miRNA expression profile of colorectal cancer (CRC), constructed a negative regulatory network of the miRNA-target genes, and identified miR-4469 as one of the key tumor suppressors miRNAs. We analyzed the expression and survival of miR-4469 in pan-cancer, experimentally verified the expression level of miR-4469 in CRC cells and the effect on CRC cell proliferation and migration. We screened miR-4469 target genes for enrichment analysis and immune cell infiltration analysis and validated target gene expression to clarify the regulatory mechanisms involved in miR-4469. RESULTS: miR-4469 was more highly expressed in normal colorectum tissues compared to CRC tissues and correlated with survival time in patients with multiple cancers. It was shown that miR-4469 was highly expressed in normal colon cells and miR-4469 expression could inhibit the proliferation and migration of CRC cells. In addition, studies on the mechanism showed that miR-4469 function is mainly related to the regulation of inflammatory cell infiltration, and the key target genes of miR-4469 in this process are SLC2A3, FGR, PLEKHO2, and MYO1F. CONCLUSION: miR-4469 is a tumor suppressor in CRC, and its regulatory mechanism mainly affects the infiltration of inflammatory cells in the cancer tissue.
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spelling pubmed-93044302022-07-25 The role of miR-4469 as a tumor suppressor regulating inflammatory cell infiltration in colorectal cancer Qi, Lu Wang, Lu Song, Fuyao Ding, Zhenhua Zhang, Ying Comput Struct Biotechnol J Research Article BACKGROUND: MicroRNA (miRNA) regulates gene expression posttranscriptionally, and some of them function in tumor suppression and can be used in drug development. As a result, identifying and screening miRNAs that suppress tumors would be a significant addition to tumor treatment. METHODS: In this study, we analyzed the miRNA expression profile of colorectal cancer (CRC), constructed a negative regulatory network of the miRNA-target genes, and identified miR-4469 as one of the key tumor suppressors miRNAs. We analyzed the expression and survival of miR-4469 in pan-cancer, experimentally verified the expression level of miR-4469 in CRC cells and the effect on CRC cell proliferation and migration. We screened miR-4469 target genes for enrichment analysis and immune cell infiltration analysis and validated target gene expression to clarify the regulatory mechanisms involved in miR-4469. RESULTS: miR-4469 was more highly expressed in normal colorectum tissues compared to CRC tissues and correlated with survival time in patients with multiple cancers. It was shown that miR-4469 was highly expressed in normal colon cells and miR-4469 expression could inhibit the proliferation and migration of CRC cells. In addition, studies on the mechanism showed that miR-4469 function is mainly related to the regulation of inflammatory cell infiltration, and the key target genes of miR-4469 in this process are SLC2A3, FGR, PLEKHO2, and MYO1F. CONCLUSION: miR-4469 is a tumor suppressor in CRC, and its regulatory mechanism mainly affects the infiltration of inflammatory cells in the cancer tissue. Research Network of Computational and Structural Biotechnology 2022-07-14 /pmc/articles/PMC9304430/ /pubmed/35891783 http://dx.doi.org/10.1016/j.csbj.2022.07.021 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Qi, Lu
Wang, Lu
Song, Fuyao
Ding, Zhenhua
Zhang, Ying
The role of miR-4469 as a tumor suppressor regulating inflammatory cell infiltration in colorectal cancer
title The role of miR-4469 as a tumor suppressor regulating inflammatory cell infiltration in colorectal cancer
title_full The role of miR-4469 as a tumor suppressor regulating inflammatory cell infiltration in colorectal cancer
title_fullStr The role of miR-4469 as a tumor suppressor regulating inflammatory cell infiltration in colorectal cancer
title_full_unstemmed The role of miR-4469 as a tumor suppressor regulating inflammatory cell infiltration in colorectal cancer
title_short The role of miR-4469 as a tumor suppressor regulating inflammatory cell infiltration in colorectal cancer
title_sort role of mir-4469 as a tumor suppressor regulating inflammatory cell infiltration in colorectal cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304430/
https://www.ncbi.nlm.nih.gov/pubmed/35891783
http://dx.doi.org/10.1016/j.csbj.2022.07.021
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