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Phenotypic Changes of Peripheral γδ T Cell and Its Subsets in Patients With Coronary Artery Disease

Coronary atherosclerotic heart disease (CAD) is a chronic inflammatory cardiovascular disease with high morbidity and mortality. Growing data indicate that many immune cells are involved in the development of atherosclerosis. However, the immunological roles of γδ T cells in the initiation and progr...

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Autores principales: Li, Yan, Jiang, Silin, Li, Jiawei, Yin, Mengzhuo, Yan, Fuxin, Chen, Yuyuan, Chen, Yan, Wu, Tongwei, Cheng, Mengliang, He, Yihua, Liang, Hongbin, Yu, Hang, Qiao, Qingqing, Guo, Zhigang, Xu, Yan, Zhang, Yanan, Xiang, Zheng, Yin, Zhinan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304556/
https://www.ncbi.nlm.nih.gov/pubmed/35874761
http://dx.doi.org/10.3389/fimmu.2022.900334
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author Li, Yan
Jiang, Silin
Li, Jiawei
Yin, Mengzhuo
Yan, Fuxin
Chen, Yuyuan
Chen, Yan
Wu, Tongwei
Cheng, Mengliang
He, Yihua
Liang, Hongbin
Yu, Hang
Qiao, Qingqing
Guo, Zhigang
Xu, Yan
Zhang, Yanan
Xiang, Zheng
Yin, Zhinan
author_facet Li, Yan
Jiang, Silin
Li, Jiawei
Yin, Mengzhuo
Yan, Fuxin
Chen, Yuyuan
Chen, Yan
Wu, Tongwei
Cheng, Mengliang
He, Yihua
Liang, Hongbin
Yu, Hang
Qiao, Qingqing
Guo, Zhigang
Xu, Yan
Zhang, Yanan
Xiang, Zheng
Yin, Zhinan
author_sort Li, Yan
collection PubMed
description Coronary atherosclerotic heart disease (CAD) is a chronic inflammatory cardiovascular disease with high morbidity and mortality. Growing data indicate that many immune cells are involved in the development of atherosclerosis. However, the immunological roles of γδ T cells in the initiation and progression of CAD are not fully understood. Here, we used flow cytometry to determine phenotypical changes of γδ T cells and their subpopulations in peripheral blood samples collected from 37 CAD patients. The Pearson correlation coefficient was used to analyze the relationship between the clinical parameter (serum LDL-C level) and the changes of immunophenotypes of γδ T cells. Our results demonstrated that the frequencies and absolute numbers of total γδ T cells and Vδ2(+) T cells were significantly decreased in CAD patients when compared to healthy individuals. However, the proportion of Vδ1(+) T cells was much lower in CAD patients than that of healthy individuals. Most importantly, a significant alteration of the Vδ1/Vδ2 ratio was found in CAD patients. In addition, a series of surface markers that are associated with costimulatory signals (CD28, CD40L, CD80, CD86), activation levels (CD69, CD25, HLA-DR), activating NK cell receptors (NKp30, NKp46, NKG2D) and inhibitory receptors (PD-1, CTLA-4, PD-1, Tim-3) were determined and then analyzed in the total γδ T cells, Vδ2(+)T cells and Vδ2(-)T cells of CAD patients and healthy individuals. The data demonstrated that immunological activities of total γδ T cells, Vδ2(+)T cells, and Vδ2(-)T cells of CAD patients were much lower than those in healthy individuals. Moreover, we found that there were positive correlations between the serum LDL-C levels and frequencies of CD3(+)γδ(+) T cells, CD69(+)Vδ2(+)T cells, NKG2D(+)Vδ2(+)T cells, and NKp46(+)Vδ2(+)T cells. By contrast, there was an inverse correlation between the levels of serum LDL-C and the frequencies of CD69(+)Vδ2(-)T cells and NKp46(+)Vδ2(-)T cells. Accordingly, these findings could help us to better understand the roles of γδ T cells in the CAD, and shed light on the development of novel diagnostic techniques and therapeutic strategies by targeting γδ T cells for CAD patients.
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spelling pubmed-93045562022-07-23 Phenotypic Changes of Peripheral γδ T Cell and Its Subsets in Patients With Coronary Artery Disease Li, Yan Jiang, Silin Li, Jiawei Yin, Mengzhuo Yan, Fuxin Chen, Yuyuan Chen, Yan Wu, Tongwei Cheng, Mengliang He, Yihua Liang, Hongbin Yu, Hang Qiao, Qingqing Guo, Zhigang Xu, Yan Zhang, Yanan Xiang, Zheng Yin, Zhinan Front Immunol Immunology Coronary atherosclerotic heart disease (CAD) is a chronic inflammatory cardiovascular disease with high morbidity and mortality. Growing data indicate that many immune cells are involved in the development of atherosclerosis. However, the immunological roles of γδ T cells in the initiation and progression of CAD are not fully understood. Here, we used flow cytometry to determine phenotypical changes of γδ T cells and their subpopulations in peripheral blood samples collected from 37 CAD patients. The Pearson correlation coefficient was used to analyze the relationship between the clinical parameter (serum LDL-C level) and the changes of immunophenotypes of γδ T cells. Our results demonstrated that the frequencies and absolute numbers of total γδ T cells and Vδ2(+) T cells were significantly decreased in CAD patients when compared to healthy individuals. However, the proportion of Vδ1(+) T cells was much lower in CAD patients than that of healthy individuals. Most importantly, a significant alteration of the Vδ1/Vδ2 ratio was found in CAD patients. In addition, a series of surface markers that are associated with costimulatory signals (CD28, CD40L, CD80, CD86), activation levels (CD69, CD25, HLA-DR), activating NK cell receptors (NKp30, NKp46, NKG2D) and inhibitory receptors (PD-1, CTLA-4, PD-1, Tim-3) were determined and then analyzed in the total γδ T cells, Vδ2(+)T cells and Vδ2(-)T cells of CAD patients and healthy individuals. The data demonstrated that immunological activities of total γδ T cells, Vδ2(+)T cells, and Vδ2(-)T cells of CAD patients were much lower than those in healthy individuals. Moreover, we found that there were positive correlations between the serum LDL-C levels and frequencies of CD3(+)γδ(+) T cells, CD69(+)Vδ2(+)T cells, NKG2D(+)Vδ2(+)T cells, and NKp46(+)Vδ2(+)T cells. By contrast, there was an inverse correlation between the levels of serum LDL-C and the frequencies of CD69(+)Vδ2(-)T cells and NKp46(+)Vδ2(-)T cells. Accordingly, these findings could help us to better understand the roles of γδ T cells in the CAD, and shed light on the development of novel diagnostic techniques and therapeutic strategies by targeting γδ T cells for CAD patients. Frontiers Media S.A. 2022-07-08 /pmc/articles/PMC9304556/ /pubmed/35874761 http://dx.doi.org/10.3389/fimmu.2022.900334 Text en Copyright © 2022 Li, Jiang, Li, Yin, Yan, Chen, Chen, Wu, Cheng, He, Liang, Yu, Qiao, Guo, Xu, Zhang, Xiang and Yin https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Li, Yan
Jiang, Silin
Li, Jiawei
Yin, Mengzhuo
Yan, Fuxin
Chen, Yuyuan
Chen, Yan
Wu, Tongwei
Cheng, Mengliang
He, Yihua
Liang, Hongbin
Yu, Hang
Qiao, Qingqing
Guo, Zhigang
Xu, Yan
Zhang, Yanan
Xiang, Zheng
Yin, Zhinan
Phenotypic Changes of Peripheral γδ T Cell and Its Subsets in Patients With Coronary Artery Disease
title Phenotypic Changes of Peripheral γδ T Cell and Its Subsets in Patients With Coronary Artery Disease
title_full Phenotypic Changes of Peripheral γδ T Cell and Its Subsets in Patients With Coronary Artery Disease
title_fullStr Phenotypic Changes of Peripheral γδ T Cell and Its Subsets in Patients With Coronary Artery Disease
title_full_unstemmed Phenotypic Changes of Peripheral γδ T Cell and Its Subsets in Patients With Coronary Artery Disease
title_short Phenotypic Changes of Peripheral γδ T Cell and Its Subsets in Patients With Coronary Artery Disease
title_sort phenotypic changes of peripheral γδ t cell and its subsets in patients with coronary artery disease
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304556/
https://www.ncbi.nlm.nih.gov/pubmed/35874761
http://dx.doi.org/10.3389/fimmu.2022.900334
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