Isolation and adoptive transfer of innate lymphoid cells 2 to a recipient mouse model of PDAC

Innate lymphoid cells 2 (ILC2) play a significant role in the tumorigenesis of pancreatic ductal adenocarcinoma (PDAC). An important aspect of ILC2-mediated tumorigenesis is the expansion of the resident ILC2 and simultaneous recruitment of the peripheral ILC2. Here, we describe a protocol for isola...

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Autores principales: Alam, Aftab, Comer, Sarah, Levanduski, Eric, Dey, Prasenjit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304675/
https://www.ncbi.nlm.nih.gov/pubmed/35874471
http://dx.doi.org/10.1016/j.xpro.2022.101563
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author Alam, Aftab
Comer, Sarah
Levanduski, Eric
Dey, Prasenjit
author_facet Alam, Aftab
Comer, Sarah
Levanduski, Eric
Dey, Prasenjit
author_sort Alam, Aftab
collection PubMed
description Innate lymphoid cells 2 (ILC2) play a significant role in the tumorigenesis of pancreatic ductal adenocarcinoma (PDAC). An important aspect of ILC2-mediated tumorigenesis is the expansion of the resident ILC2 and simultaneous recruitment of the peripheral ILC2. Here, we describe a protocol for isolation, enrichment, and DiD labeling of ILC2 for in vivo tracking of ILC2s in the mouse. Further, we describe steps for the adoptive transfer of ILC2 to a recipient mouse model of PDAC. For complete details on the use and execution of this protocol, please refer to Alam et al. (2022).
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spelling pubmed-93046752022-07-23 Isolation and adoptive transfer of innate lymphoid cells 2 to a recipient mouse model of PDAC Alam, Aftab Comer, Sarah Levanduski, Eric Dey, Prasenjit STAR Protoc Protocol Innate lymphoid cells 2 (ILC2) play a significant role in the tumorigenesis of pancreatic ductal adenocarcinoma (PDAC). An important aspect of ILC2-mediated tumorigenesis is the expansion of the resident ILC2 and simultaneous recruitment of the peripheral ILC2. Here, we describe a protocol for isolation, enrichment, and DiD labeling of ILC2 for in vivo tracking of ILC2s in the mouse. Further, we describe steps for the adoptive transfer of ILC2 to a recipient mouse model of PDAC. For complete details on the use and execution of this protocol, please refer to Alam et al. (2022). Elsevier 2022-07-18 /pmc/articles/PMC9304675/ /pubmed/35874471 http://dx.doi.org/10.1016/j.xpro.2022.101563 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Alam, Aftab
Comer, Sarah
Levanduski, Eric
Dey, Prasenjit
Isolation and adoptive transfer of innate lymphoid cells 2 to a recipient mouse model of PDAC
title Isolation and adoptive transfer of innate lymphoid cells 2 to a recipient mouse model of PDAC
title_full Isolation and adoptive transfer of innate lymphoid cells 2 to a recipient mouse model of PDAC
title_fullStr Isolation and adoptive transfer of innate lymphoid cells 2 to a recipient mouse model of PDAC
title_full_unstemmed Isolation and adoptive transfer of innate lymphoid cells 2 to a recipient mouse model of PDAC
title_short Isolation and adoptive transfer of innate lymphoid cells 2 to a recipient mouse model of PDAC
title_sort isolation and adoptive transfer of innate lymphoid cells 2 to a recipient mouse model of pdac
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304675/
https://www.ncbi.nlm.nih.gov/pubmed/35874471
http://dx.doi.org/10.1016/j.xpro.2022.101563
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