Isolation of tumor-infiltrating lymphocytes from preserved human tumor tissue specimens for downstream characterization

Immunophenotyping of tumor-infiltrating lymphocytes (TILs) by flow cytometry can predict clinical efficacy of immunotherapy. However, several obstacles need to be overcome for developing a flow cytometry assay starting from solid tumor specimens. Here, we show a detailed enzyme-based protocol to iso...

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Detalles Bibliográficos
Autores principales: Kobayashi, Tamiyo, Kumagai, Shogo, Doi, Rieko, Afonina, Elena, Koyama, Shohei, Nishikawa, Hiroyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304678/
https://www.ncbi.nlm.nih.gov/pubmed/35852944
http://dx.doi.org/10.1016/j.xpro.2022.101557
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author Kobayashi, Tamiyo
Kumagai, Shogo
Doi, Rieko
Afonina, Elena
Koyama, Shohei
Nishikawa, Hiroyoshi
author_facet Kobayashi, Tamiyo
Kumagai, Shogo
Doi, Rieko
Afonina, Elena
Koyama, Shohei
Nishikawa, Hiroyoshi
author_sort Kobayashi, Tamiyo
collection PubMed
description Immunophenotyping of tumor-infiltrating lymphocytes (TILs) by flow cytometry can predict clinical efficacy of immunotherapy. However, several obstacles need to be overcome for developing a flow cytometry assay starting from solid tumor specimens. Here, we show a detailed enzyme-based protocol to isolate TILs from human tumor tissues. The protocol was optimized to obtain enough viable TILs from a biopsy tissue specimen for flow cytometry-based TIL immunophenotyping. Additionally, tissue samples could be preserved for up to 72 h for subsequent characterization. For complete details on the use and execution of this protocol, please refer to Kumagai et al. (2020, 2022).
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spelling pubmed-93046782022-07-23 Isolation of tumor-infiltrating lymphocytes from preserved human tumor tissue specimens for downstream characterization Kobayashi, Tamiyo Kumagai, Shogo Doi, Rieko Afonina, Elena Koyama, Shohei Nishikawa, Hiroyoshi STAR Protoc Protocol Immunophenotyping of tumor-infiltrating lymphocytes (TILs) by flow cytometry can predict clinical efficacy of immunotherapy. However, several obstacles need to be overcome for developing a flow cytometry assay starting from solid tumor specimens. Here, we show a detailed enzyme-based protocol to isolate TILs from human tumor tissues. The protocol was optimized to obtain enough viable TILs from a biopsy tissue specimen for flow cytometry-based TIL immunophenotyping. Additionally, tissue samples could be preserved for up to 72 h for subsequent characterization. For complete details on the use and execution of this protocol, please refer to Kumagai et al. (2020, 2022). Elsevier 2022-07-18 /pmc/articles/PMC9304678/ /pubmed/35852944 http://dx.doi.org/10.1016/j.xpro.2022.101557 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Kobayashi, Tamiyo
Kumagai, Shogo
Doi, Rieko
Afonina, Elena
Koyama, Shohei
Nishikawa, Hiroyoshi
Isolation of tumor-infiltrating lymphocytes from preserved human tumor tissue specimens for downstream characterization
title Isolation of tumor-infiltrating lymphocytes from preserved human tumor tissue specimens for downstream characterization
title_full Isolation of tumor-infiltrating lymphocytes from preserved human tumor tissue specimens for downstream characterization
title_fullStr Isolation of tumor-infiltrating lymphocytes from preserved human tumor tissue specimens for downstream characterization
title_full_unstemmed Isolation of tumor-infiltrating lymphocytes from preserved human tumor tissue specimens for downstream characterization
title_short Isolation of tumor-infiltrating lymphocytes from preserved human tumor tissue specimens for downstream characterization
title_sort isolation of tumor-infiltrating lymphocytes from preserved human tumor tissue specimens for downstream characterization
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304678/
https://www.ncbi.nlm.nih.gov/pubmed/35852944
http://dx.doi.org/10.1016/j.xpro.2022.101557
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