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Isolation of tumor-infiltrating lymphocytes from preserved human tumor tissue specimens for downstream characterization

Immunophenotyping of tumor-infiltrating lymphocytes (TILs) by flow cytometry can predict clinical efficacy of immunotherapy. However, several obstacles need to be overcome for developing a flow cytometry assay starting from solid tumor specimens. Here, we show a detailed enzyme-based protocol to iso...

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Detalles Bibliográficos
Autores principales:	Kobayashi, Tamiyo, Kumagai, Shogo, Doi, Rieko, Afonina, Elena, Koyama, Shohei, Nishikawa, Hiroyoshi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304678/ https://www.ncbi.nlm.nih.gov/pubmed/35852944 http://dx.doi.org/10.1016/j.xpro.2022.101557

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