Protocol for establishing a protein-protein interaction network using tandem affinity purification followed by mass spectrometry in mammalian cells

Identification of protein interactors is fundamental to understanding their functions. Here, we describe a modified protocol for tandem affinity purification coupled with mass spectrometry (TAP/MS), which includes two-step purification. We detail the S-, 2×FLAG-, and Streptavidin-Binding Peptide (SB...

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Detalles Bibliográficos
Autores principales: Bian, Weixiang, Jiang, Hua, Feng, Shan, Chen, Junjie, Wang, Wenqi, Li, Xu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304681/
https://www.ncbi.nlm.nih.gov/pubmed/35874475
http://dx.doi.org/10.1016/j.xpro.2022.101569

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