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The medial prefrontal cortex to the medial amygdala connections may affect the anxiety level in aged rats

BACKGROUND: Aging changes brain function and behavior differently in male and female individuals. Changes in the medial prefrontal cortex (mPFC)–medial amygdala (MeA) connectivity affect anxiety‐like behavior. OBJECTIVES: Therefore, this study aimed to investigate the effect of aging and sex on the...

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Autores principales: Sotoudeh, Narges, Namavar, Mohammad Reza, Bagheri, Farshid, Zarifkar, Asadollah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304845/
https://www.ncbi.nlm.nih.gov/pubmed/35605044
http://dx.doi.org/10.1002/brb3.2616
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author Sotoudeh, Narges
Namavar, Mohammad Reza
Bagheri, Farshid
Zarifkar, Asadollah
author_facet Sotoudeh, Narges
Namavar, Mohammad Reza
Bagheri, Farshid
Zarifkar, Asadollah
author_sort Sotoudeh, Narges
collection PubMed
description BACKGROUND: Aging changes brain function and behavior differently in male and female individuals. Changes in the medial prefrontal cortex (mPFC)–medial amygdala (MeA) connectivity affect anxiety‐like behavior. OBJECTIVES: Therefore, this study aimed to investigate the effect of aging and sex on the mPFC–MeA connection and its association with the level of anxiety‐like behavior. METHODS: We divided the Wistar rats into the male and female young rats (2‐3‐month‐old) and male and female old rats (18–20 months old). First, the open field test (OFT) was performed, and then 80 nl of Fluoro‐Gold (FG) was injected by stereotaxic surgery in the right or left MeA. After 10 days, the animals were perfused, their brain removed, coronal sections cut, and the number of FG‐labeled cells in the right and left mPFC of each sample was estimated. RESULTS: Based on our results, old animals revealed less anxiety‐like behavior than young ones, and young females were less anxious than young males, too. Interestingly, MeA of old male rats received more fibers from the bilateral mPFC than young ones. Also, this connection was stronger in the young females than young males. Altogether, the present study indicated that old individuals had less anxiety‐like behavior and stronger mPFC–MeA connection, and young female rats were less anxious and had a stronger connection of mPFC–amygdala than males of the same age. CONCLUSION: Thus, it seems that there is a negative relationship between anxiety levels based on the rat's performance in the OFT apparatus and the mPFC–MeA connection.
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spelling pubmed-93048452022-07-26 The medial prefrontal cortex to the medial amygdala connections may affect the anxiety level in aged rats Sotoudeh, Narges Namavar, Mohammad Reza Bagheri, Farshid Zarifkar, Asadollah Brain Behav Original Articles BACKGROUND: Aging changes brain function and behavior differently in male and female individuals. Changes in the medial prefrontal cortex (mPFC)–medial amygdala (MeA) connectivity affect anxiety‐like behavior. OBJECTIVES: Therefore, this study aimed to investigate the effect of aging and sex on the mPFC–MeA connection and its association with the level of anxiety‐like behavior. METHODS: We divided the Wistar rats into the male and female young rats (2‐3‐month‐old) and male and female old rats (18–20 months old). First, the open field test (OFT) was performed, and then 80 nl of Fluoro‐Gold (FG) was injected by stereotaxic surgery in the right or left MeA. After 10 days, the animals were perfused, their brain removed, coronal sections cut, and the number of FG‐labeled cells in the right and left mPFC of each sample was estimated. RESULTS: Based on our results, old animals revealed less anxiety‐like behavior than young ones, and young females were less anxious than young males, too. Interestingly, MeA of old male rats received more fibers from the bilateral mPFC than young ones. Also, this connection was stronger in the young females than young males. Altogether, the present study indicated that old individuals had less anxiety‐like behavior and stronger mPFC–MeA connection, and young female rats were less anxious and had a stronger connection of mPFC–amygdala than males of the same age. CONCLUSION: Thus, it seems that there is a negative relationship between anxiety levels based on the rat's performance in the OFT apparatus and the mPFC–MeA connection. John Wiley and Sons Inc. 2022-05-23 /pmc/articles/PMC9304845/ /pubmed/35605044 http://dx.doi.org/10.1002/brb3.2616 Text en © 2022 The Authors. Brain and Behavior published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Sotoudeh, Narges
Namavar, Mohammad Reza
Bagheri, Farshid
Zarifkar, Asadollah
The medial prefrontal cortex to the medial amygdala connections may affect the anxiety level in aged rats
title The medial prefrontal cortex to the medial amygdala connections may affect the anxiety level in aged rats
title_full The medial prefrontal cortex to the medial amygdala connections may affect the anxiety level in aged rats
title_fullStr The medial prefrontal cortex to the medial amygdala connections may affect the anxiety level in aged rats
title_full_unstemmed The medial prefrontal cortex to the medial amygdala connections may affect the anxiety level in aged rats
title_short The medial prefrontal cortex to the medial amygdala connections may affect the anxiety level in aged rats
title_sort medial prefrontal cortex to the medial amygdala connections may affect the anxiety level in aged rats
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304845/
https://www.ncbi.nlm.nih.gov/pubmed/35605044
http://dx.doi.org/10.1002/brb3.2616
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