Cargando…

Artemisia annua L. Aqueous Extract Promotes Intestine Immunity and Antioxidant Function in Broilers

This study was conducted to investigate the effects of Artemisia annua L. aqueous extract (AAE) on intestinal immune and antioxidative function of broilers. A total of 200 one-day-old Arbor Acre broilers were randomly allotted into five dietary treatment groups, with five replicates per treatment an...

Descripción completa

Detalles Bibliográficos
Autores principales: Guo, Shiwei, Ma, Jiaxin, Xing, Yuanyuan, Shi, Lulu, Zhang, Linghui, Xu, Yuanqing, Jin, Xiao, Yan, Sumei, Shi, Binlin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304935/
https://www.ncbi.nlm.nih.gov/pubmed/35873687
http://dx.doi.org/10.3389/fvets.2022.934021
_version_ 1784752203513200640
author Guo, Shiwei
Ma, Jiaxin
Xing, Yuanyuan
Shi, Lulu
Zhang, Linghui
Xu, Yuanqing
Jin, Xiao
Yan, Sumei
Shi, Binlin
author_facet Guo, Shiwei
Ma, Jiaxin
Xing, Yuanyuan
Shi, Lulu
Zhang, Linghui
Xu, Yuanqing
Jin, Xiao
Yan, Sumei
Shi, Binlin
author_sort Guo, Shiwei
collection PubMed
description This study was conducted to investigate the effects of Artemisia annua L. aqueous extract (AAE) on intestinal immune and antioxidative function of broilers. A total of 200 one-day-old Arbor Acre broilers were randomly allotted into five dietary treatment groups, with five replicates per treatment and eight broilers per replicate. The five treatment diets were formulated by adding, respectively, 0 (control group), 0.5, 1.0, 1.5, and 2.0 g/kg AAE in the basal diet. The results showed that dietary inclusion of AAE quadratically decreased interleukin (IL)-1β content, linearly decreased IL-6 content in the small intestine through regulating the nuclear factor-kappa B signal pathway, and quadratically increased immunoglobulin (Ig)M and sIgA content in ileum and jejunum. Besides, there was a quadratic decrease in the gene expression of IL-1β, IL-6, and toll like receptor 4 (TLR4) in ileum on day 21, and the gene expression of IL-6 and TLR4 in duodenum on day 42, thereby improving small intestinal immune function in broilers. Additionally, dietary inclusion of AAE improves antioxidative function through the nuclear factor-erythroid 2-related factor 2 (Nrf2) signal pathway in the small intestinal mucosa of broilers, especially, quadratically increased catalase (CAT) and superoxidase dismutase activity in ileum, and total antioxidant capacity and glutathione peroxidase activity in duodenum, and quadratically decreased malondialdehyde concentration in ileum, besides, linearly increased heme oxygenase-1 and Nrf2 gene expression in jejunum and ileum on day 42, quadratically increased CAT gene expression in the small intestine. Furthermore, regression analyses of the above parameters showed that the optimal dose range of AAE in the diet of broilers was 1.12–1.38 g/kg.
format Online
Article
Text
id pubmed-9304935
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-93049352022-07-23 Artemisia annua L. Aqueous Extract Promotes Intestine Immunity and Antioxidant Function in Broilers Guo, Shiwei Ma, Jiaxin Xing, Yuanyuan Shi, Lulu Zhang, Linghui Xu, Yuanqing Jin, Xiao Yan, Sumei Shi, Binlin Front Vet Sci Veterinary Science This study was conducted to investigate the effects of Artemisia annua L. aqueous extract (AAE) on intestinal immune and antioxidative function of broilers. A total of 200 one-day-old Arbor Acre broilers were randomly allotted into five dietary treatment groups, with five replicates per treatment and eight broilers per replicate. The five treatment diets were formulated by adding, respectively, 0 (control group), 0.5, 1.0, 1.5, and 2.0 g/kg AAE in the basal diet. The results showed that dietary inclusion of AAE quadratically decreased interleukin (IL)-1β content, linearly decreased IL-6 content in the small intestine through regulating the nuclear factor-kappa B signal pathway, and quadratically increased immunoglobulin (Ig)M and sIgA content in ileum and jejunum. Besides, there was a quadratic decrease in the gene expression of IL-1β, IL-6, and toll like receptor 4 (TLR4) in ileum on day 21, and the gene expression of IL-6 and TLR4 in duodenum on day 42, thereby improving small intestinal immune function in broilers. Additionally, dietary inclusion of AAE improves antioxidative function through the nuclear factor-erythroid 2-related factor 2 (Nrf2) signal pathway in the small intestinal mucosa of broilers, especially, quadratically increased catalase (CAT) and superoxidase dismutase activity in ileum, and total antioxidant capacity and glutathione peroxidase activity in duodenum, and quadratically decreased malondialdehyde concentration in ileum, besides, linearly increased heme oxygenase-1 and Nrf2 gene expression in jejunum and ileum on day 42, quadratically increased CAT gene expression in the small intestine. Furthermore, regression analyses of the above parameters showed that the optimal dose range of AAE in the diet of broilers was 1.12–1.38 g/kg. Frontiers Media S.A. 2022-07-08 /pmc/articles/PMC9304935/ /pubmed/35873687 http://dx.doi.org/10.3389/fvets.2022.934021 Text en Copyright © 2022 Guo, Ma, Xing, Shi, Zhang, Xu, Jin, Yan and Shi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Guo, Shiwei
Ma, Jiaxin
Xing, Yuanyuan
Shi, Lulu
Zhang, Linghui
Xu, Yuanqing
Jin, Xiao
Yan, Sumei
Shi, Binlin
Artemisia annua L. Aqueous Extract Promotes Intestine Immunity and Antioxidant Function in Broilers
title Artemisia annua L. Aqueous Extract Promotes Intestine Immunity and Antioxidant Function in Broilers
title_full Artemisia annua L. Aqueous Extract Promotes Intestine Immunity and Antioxidant Function in Broilers
title_fullStr Artemisia annua L. Aqueous Extract Promotes Intestine Immunity and Antioxidant Function in Broilers
title_full_unstemmed Artemisia annua L. Aqueous Extract Promotes Intestine Immunity and Antioxidant Function in Broilers
title_short Artemisia annua L. Aqueous Extract Promotes Intestine Immunity and Antioxidant Function in Broilers
title_sort artemisia annua l. aqueous extract promotes intestine immunity and antioxidant function in broilers
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304935/
https://www.ncbi.nlm.nih.gov/pubmed/35873687
http://dx.doi.org/10.3389/fvets.2022.934021
work_keys_str_mv AT guoshiwei artemisiaannualaqueousextractpromotesintestineimmunityandantioxidantfunctioninbroilers
AT majiaxin artemisiaannualaqueousextractpromotesintestineimmunityandantioxidantfunctioninbroilers
AT xingyuanyuan artemisiaannualaqueousextractpromotesintestineimmunityandantioxidantfunctioninbroilers
AT shilulu artemisiaannualaqueousextractpromotesintestineimmunityandantioxidantfunctioninbroilers
AT zhanglinghui artemisiaannualaqueousextractpromotesintestineimmunityandantioxidantfunctioninbroilers
AT xuyuanqing artemisiaannualaqueousextractpromotesintestineimmunityandantioxidantfunctioninbroilers
AT jinxiao artemisiaannualaqueousextractpromotesintestineimmunityandantioxidantfunctioninbroilers
AT yansumei artemisiaannualaqueousextractpromotesintestineimmunityandantioxidantfunctioninbroilers
AT shibinlin artemisiaannualaqueousextractpromotesintestineimmunityandantioxidantfunctioninbroilers