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Prospects of Coffee Leaf against SARS-CoV-2 Infection
In the current climate, many countries are in dire need of effective preventive methods to curb the Severe Acute Respiratory Syndrome Coronavirus Type 2 (SARS-CoV-2) pandemic. The purpose of this research is to screen and explore natural plant extracts that have the potential to against SARS-CoV-2 a...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305275/ https://www.ncbi.nlm.nih.gov/pubmed/35874948 http://dx.doi.org/10.7150/ijbs.76058 |
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author | Wu, Chen-Shiou Chiang, Hsiu-Mei Chen, Yeh Chen, Chung-Yu Chen, Hsiao-Fan Su, Wen-Chi Wang, Wei-Jan Chou, Yu-Chi Chang, Wei-Chao Wang, Shao-Chun Hung, Mien-Chie |
author_facet | Wu, Chen-Shiou Chiang, Hsiu-Mei Chen, Yeh Chen, Chung-Yu Chen, Hsiao-Fan Su, Wen-Chi Wang, Wei-Jan Chou, Yu-Chi Chang, Wei-Chao Wang, Shao-Chun Hung, Mien-Chie |
author_sort | Wu, Chen-Shiou |
collection | PubMed |
description | In the current climate, many countries are in dire need of effective preventive methods to curb the Severe Acute Respiratory Syndrome Coronavirus Type 2 (SARS-CoV-2) pandemic. The purpose of this research is to screen and explore natural plant extracts that have the potential to against SARS-CoV-2 and provide alternative options for SARS-CoV-2 prevention and hand sanitizer or spray-like disinfectants. We first used Spike-ACE2 ELISA and TMPRSS2 fluorescence resonance energy transfer (FRET) assays to screen extracts from agricultural by-products from Taiwan with the potential to impede SARS-CoV-2 infection. Next, the SARS-CoV-2 pseudo-particles (Vpp) infection assay was tested to validate the effectiveness. We identified an extract from coffee leaf (Coffea Arabica), a natural plant that effectively inhibited wild-type SARS-CoV-2, and five Variants of Concern (Alpha, Beta, Gamma, Delta, and Omicron strain) from entering host cells. In an attempt to apply coffee leaf extract for hand sanitizer or spray-like disinfectants, we designed a skin-like gelatin membrane experiment. We showed that the high concentration of coffee leaf extract on the skin surface could block SARS-CoV-2 into cells more potently than 75% Ethanol, a standard disinfectant to inactivate SARS-CoV-2. Finally, LC-HRMS analysis was used to identify compounds such as caffeine, chlorogenic acid (CGA), quinic acid, and mangiferin that are associated with an anti-SARS-CoV-2 activity. Our results demonstrated that coffee leaf extract, an agricultural by-product effectively inhibits SARS-CoV-2 Vpp infection through an ACE2-dependent mechanism and may be utilized to develop products against SARS-CoV-2 infection. |
format | Online Article Text |
id | pubmed-9305275 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-93052752022-07-22 Prospects of Coffee Leaf against SARS-CoV-2 Infection Wu, Chen-Shiou Chiang, Hsiu-Mei Chen, Yeh Chen, Chung-Yu Chen, Hsiao-Fan Su, Wen-Chi Wang, Wei-Jan Chou, Yu-Chi Chang, Wei-Chao Wang, Shao-Chun Hung, Mien-Chie Int J Biol Sci Research Paper In the current climate, many countries are in dire need of effective preventive methods to curb the Severe Acute Respiratory Syndrome Coronavirus Type 2 (SARS-CoV-2) pandemic. The purpose of this research is to screen and explore natural plant extracts that have the potential to against SARS-CoV-2 and provide alternative options for SARS-CoV-2 prevention and hand sanitizer or spray-like disinfectants. We first used Spike-ACE2 ELISA and TMPRSS2 fluorescence resonance energy transfer (FRET) assays to screen extracts from agricultural by-products from Taiwan with the potential to impede SARS-CoV-2 infection. Next, the SARS-CoV-2 pseudo-particles (Vpp) infection assay was tested to validate the effectiveness. We identified an extract from coffee leaf (Coffea Arabica), a natural plant that effectively inhibited wild-type SARS-CoV-2, and five Variants of Concern (Alpha, Beta, Gamma, Delta, and Omicron strain) from entering host cells. In an attempt to apply coffee leaf extract for hand sanitizer or spray-like disinfectants, we designed a skin-like gelatin membrane experiment. We showed that the high concentration of coffee leaf extract on the skin surface could block SARS-CoV-2 into cells more potently than 75% Ethanol, a standard disinfectant to inactivate SARS-CoV-2. Finally, LC-HRMS analysis was used to identify compounds such as caffeine, chlorogenic acid (CGA), quinic acid, and mangiferin that are associated with an anti-SARS-CoV-2 activity. Our results demonstrated that coffee leaf extract, an agricultural by-product effectively inhibits SARS-CoV-2 Vpp infection through an ACE2-dependent mechanism and may be utilized to develop products against SARS-CoV-2 infection. Ivyspring International Publisher 2022-07-11 /pmc/articles/PMC9305275/ /pubmed/35874948 http://dx.doi.org/10.7150/ijbs.76058 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Wu, Chen-Shiou Chiang, Hsiu-Mei Chen, Yeh Chen, Chung-Yu Chen, Hsiao-Fan Su, Wen-Chi Wang, Wei-Jan Chou, Yu-Chi Chang, Wei-Chao Wang, Shao-Chun Hung, Mien-Chie Prospects of Coffee Leaf against SARS-CoV-2 Infection |
title | Prospects of Coffee Leaf against SARS-CoV-2 Infection |
title_full | Prospects of Coffee Leaf against SARS-CoV-2 Infection |
title_fullStr | Prospects of Coffee Leaf against SARS-CoV-2 Infection |
title_full_unstemmed | Prospects of Coffee Leaf against SARS-CoV-2 Infection |
title_short | Prospects of Coffee Leaf against SARS-CoV-2 Infection |
title_sort | prospects of coffee leaf against sars-cov-2 infection |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305275/ https://www.ncbi.nlm.nih.gov/pubmed/35874948 http://dx.doi.org/10.7150/ijbs.76058 |
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