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Combining DNA Barcoding and Chemical fingerprints to authenticate Lavender raw material

OBJECTIVE: This study was initiated and conducted by several laboratories, 3 of the main cosmetic ingredient suppliers and 4 brands of cosmetics in France. Its objective is to show the interest and robustness of coupling chemical and genetic analyses in the identification of plant species. In this s...

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Autores principales: Philippe, Florian, Dubrulle, Nelly, Marteaux, Benjamin, Bonnet, Brice, Choisy, Patrick, Berthon, Jean‐Yves, Garnier, Laurence, Leconte, Nadine, Milesi, Sandrine, Morvan, Pierre‐Yves, Saunois, Alex, Sun, Jian‐Sheng, Weber, Sandrine, Giraud, Nicole
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305429/
https://www.ncbi.nlm.nih.gov/pubmed/34860432
http://dx.doi.org/10.1111/ics.12757
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author Philippe, Florian
Dubrulle, Nelly
Marteaux, Benjamin
Bonnet, Brice
Choisy, Patrick
Berthon, Jean‐Yves
Garnier, Laurence
Leconte, Nadine
Milesi, Sandrine
Morvan, Pierre‐Yves
Saunois, Alex
Sun, Jian‐Sheng
Weber, Sandrine
Giraud, Nicole
author_facet Philippe, Florian
Dubrulle, Nelly
Marteaux, Benjamin
Bonnet, Brice
Choisy, Patrick
Berthon, Jean‐Yves
Garnier, Laurence
Leconte, Nadine
Milesi, Sandrine
Morvan, Pierre‐Yves
Saunois, Alex
Sun, Jian‐Sheng
Weber, Sandrine
Giraud, Nicole
author_sort Philippe, Florian
collection PubMed
description OBJECTIVE: This study was initiated and conducted by several laboratories, 3 of the main cosmetic ingredient suppliers and 4 brands of cosmetics in France. Its objective is to show the interest and robustness of coupling chemical and genetic analyses in the identification of plant species. In this study, the Lavandula genus was used. METHODS: In this study, we used two analytical methods. Chemical analysis from UHPLC (ultra‐high‐performance liquid chromatography) and genetic analysis from barcoding with genetic markers. RESULTS: Eleven lavender species were selected (botanically authenticated) and analysed. The results show that three chemical compounds (coumaric acid hexoside, ferulic acid hexoside and rosmarinic acid) and three genetic markers (RbcL, trnH‐psbA and ITS) are of interest for the differentiation of species of the genus lavandula. CONCLUSION: The results show that the combination of complementary analytical methods is a relevant system to prove the botanical identification of lavender species. This first study, carried out on a plant of interest for cosmetics, demonstrates the need for authentication using a tool combining genetic and chemical analysis as an advance over traditional investigation methods used alone, in terms of identification and authentication reliability.
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spelling pubmed-93054292022-07-28 Combining DNA Barcoding and Chemical fingerprints to authenticate Lavender raw material Philippe, Florian Dubrulle, Nelly Marteaux, Benjamin Bonnet, Brice Choisy, Patrick Berthon, Jean‐Yves Garnier, Laurence Leconte, Nadine Milesi, Sandrine Morvan, Pierre‐Yves Saunois, Alex Sun, Jian‐Sheng Weber, Sandrine Giraud, Nicole Int J Cosmet Sci Original Articles OBJECTIVE: This study was initiated and conducted by several laboratories, 3 of the main cosmetic ingredient suppliers and 4 brands of cosmetics in France. Its objective is to show the interest and robustness of coupling chemical and genetic analyses in the identification of plant species. In this study, the Lavandula genus was used. METHODS: In this study, we used two analytical methods. Chemical analysis from UHPLC (ultra‐high‐performance liquid chromatography) and genetic analysis from barcoding with genetic markers. RESULTS: Eleven lavender species were selected (botanically authenticated) and analysed. The results show that three chemical compounds (coumaric acid hexoside, ferulic acid hexoside and rosmarinic acid) and three genetic markers (RbcL, trnH‐psbA and ITS) are of interest for the differentiation of species of the genus lavandula. CONCLUSION: The results show that the combination of complementary analytical methods is a relevant system to prove the botanical identification of lavender species. This first study, carried out on a plant of interest for cosmetics, demonstrates the need for authentication using a tool combining genetic and chemical analysis as an advance over traditional investigation methods used alone, in terms of identification and authentication reliability. John Wiley and Sons Inc. 2021-12-23 2022-02 /pmc/articles/PMC9305429/ /pubmed/34860432 http://dx.doi.org/10.1111/ics.12757 Text en © 2021 The Authors. International Journal of Cosmetic Science published by John Wiley & Sons Ltd on behalf of Society of Cosmetic Scientists and Societe Francaise de Cosmetologie. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Philippe, Florian
Dubrulle, Nelly
Marteaux, Benjamin
Bonnet, Brice
Choisy, Patrick
Berthon, Jean‐Yves
Garnier, Laurence
Leconte, Nadine
Milesi, Sandrine
Morvan, Pierre‐Yves
Saunois, Alex
Sun, Jian‐Sheng
Weber, Sandrine
Giraud, Nicole
Combining DNA Barcoding and Chemical fingerprints to authenticate Lavender raw material
title Combining DNA Barcoding and Chemical fingerprints to authenticate Lavender raw material
title_full Combining DNA Barcoding and Chemical fingerprints to authenticate Lavender raw material
title_fullStr Combining DNA Barcoding and Chemical fingerprints to authenticate Lavender raw material
title_full_unstemmed Combining DNA Barcoding and Chemical fingerprints to authenticate Lavender raw material
title_short Combining DNA Barcoding and Chemical fingerprints to authenticate Lavender raw material
title_sort combining dna barcoding and chemical fingerprints to authenticate lavender raw material
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305429/
https://www.ncbi.nlm.nih.gov/pubmed/34860432
http://dx.doi.org/10.1111/ics.12757
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