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Plants utilise ancient conserved peptide upstream open reading frames in stress‐responsive translational regulation
The regulation of protein synthesis plays an important role in the growth and development of all organisms. Upstream open reading frames (uORFs) are commonly found in eukaryotic messenger RNA transcripts and typically attenuate the translation of associated downstream main ORFs (mORFs). Conserved pe...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305500/ https://www.ncbi.nlm.nih.gov/pubmed/35128674 http://dx.doi.org/10.1111/pce.14277 |
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author | Causier, Barry Hopes, Tayah McKay, Mary Paling, Zachary Davies, Brendan |
author_facet | Causier, Barry Hopes, Tayah McKay, Mary Paling, Zachary Davies, Brendan |
author_sort | Causier, Barry |
collection | PubMed |
description | The regulation of protein synthesis plays an important role in the growth and development of all organisms. Upstream open reading frames (uORFs) are commonly found in eukaryotic messenger RNA transcripts and typically attenuate the translation of associated downstream main ORFs (mORFs). Conserved peptide uORFs (CPuORFs) are a rare subset of uORFs, some of which have been shown to conditionally regulate translation by ribosome stalling. Here, we show that Arabidopsis CPuORF19, CPuORF46 and CPuORF47, which are ancient in origin, regulate translation of any downstream ORF, in response to the agriculturally significant environmental signals, heat stress and water limitation. Consequently, these CPuORFs represent a versatile toolkit for inducible gene expression with broad applications. Finally, we note that different classes of CPuORFs may operate during distinct phases of translation, which has implications for the bioengineering of these regulatory factors. |
format | Online Article Text |
id | pubmed-9305500 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93055002022-07-28 Plants utilise ancient conserved peptide upstream open reading frames in stress‐responsive translational regulation Causier, Barry Hopes, Tayah McKay, Mary Paling, Zachary Davies, Brendan Plant Cell Environ Original Articles The regulation of protein synthesis plays an important role in the growth and development of all organisms. Upstream open reading frames (uORFs) are commonly found in eukaryotic messenger RNA transcripts and typically attenuate the translation of associated downstream main ORFs (mORFs). Conserved peptide uORFs (CPuORFs) are a rare subset of uORFs, some of which have been shown to conditionally regulate translation by ribosome stalling. Here, we show that Arabidopsis CPuORF19, CPuORF46 and CPuORF47, which are ancient in origin, regulate translation of any downstream ORF, in response to the agriculturally significant environmental signals, heat stress and water limitation. Consequently, these CPuORFs represent a versatile toolkit for inducible gene expression with broad applications. Finally, we note that different classes of CPuORFs may operate during distinct phases of translation, which has implications for the bioengineering of these regulatory factors. John Wiley and Sons Inc. 2022-02-15 2022-04 /pmc/articles/PMC9305500/ /pubmed/35128674 http://dx.doi.org/10.1111/pce.14277 Text en © 2022 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Causier, Barry Hopes, Tayah McKay, Mary Paling, Zachary Davies, Brendan Plants utilise ancient conserved peptide upstream open reading frames in stress‐responsive translational regulation |
title | Plants utilise ancient conserved peptide upstream open reading frames in stress‐responsive translational regulation |
title_full | Plants utilise ancient conserved peptide upstream open reading frames in stress‐responsive translational regulation |
title_fullStr | Plants utilise ancient conserved peptide upstream open reading frames in stress‐responsive translational regulation |
title_full_unstemmed | Plants utilise ancient conserved peptide upstream open reading frames in stress‐responsive translational regulation |
title_short | Plants utilise ancient conserved peptide upstream open reading frames in stress‐responsive translational regulation |
title_sort | plants utilise ancient conserved peptide upstream open reading frames in stress‐responsive translational regulation |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305500/ https://www.ncbi.nlm.nih.gov/pubmed/35128674 http://dx.doi.org/10.1111/pce.14277 |
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