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Asymmetric expression of proteins in the granules of the placentomal Binucleate cells in Giraffa camelopardalis
Mature granulated trophoblast binucleate cells (BNC) have been found in all ruminant placentas examined histologically so far. BNC are normally fairly evenly distributed throughout the fetal villus and all their granules contain a similar variety of hormones and pregnancy associated glycoproteins (P...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305501/ https://www.ncbi.nlm.nih.gov/pubmed/35040958 http://dx.doi.org/10.1093/biolre/ioab247 |
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author | Wooding, F B P Forhead, A J Wilsher, S Allen, W R Roberts, R M Green, J A Beckers, J F Sousa, N Melo Charpigny, G |
author_facet | Wooding, F B P Forhead, A J Wilsher, S Allen, W R Roberts, R M Green, J A Beckers, J F Sousa, N Melo Charpigny, G |
author_sort | Wooding, F B P |
collection | PubMed |
description | Mature granulated trophoblast binucleate cells (BNC) have been found in all ruminant placentas examined histologically so far. BNC are normally fairly evenly distributed throughout the fetal villus and all their granules contain a similar variety of hormones and pregnancy associated glycoproteins (PAGs). Only the Giraffe is reported to show a different BNC protein expression, this paper is designed to investigate that. Gold labelled Lectin histochemistry and protein immunocytochemistry were used on deplasticised 1 μm sections of a wide variety of ruminant placentomes with a wide range of antibodies and lectins. In the Giraffe placentomes, even though the lectin histochemistry shows an even distribution of BNC throughout the trophoblast of the placental villi, the protein expression in the BNC granules is limited to the BNC either in the apex or the base of the villi. Placental lactogens and Prolactin (PRL) are present only in basally situated BNC: PAGs only in the apical BNC. PRL is only found in the Giraffe BNC which react with many fewer of the wide range of antibodies used here to investigate the uniformity of protein expression in ruminant BNC. The possible relevance of these differences to ruminant function and evolution is considered to provide a further example of the versatility of the BNC system. |
format | Online Article Text |
id | pubmed-9305501 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-93055012022-07-22 Asymmetric expression of proteins in the granules of the placentomal Binucleate cells in Giraffa camelopardalis Wooding, F B P Forhead, A J Wilsher, S Allen, W R Roberts, R M Green, J A Beckers, J F Sousa, N Melo Charpigny, G Biol Reprod Research Article Mature granulated trophoblast binucleate cells (BNC) have been found in all ruminant placentas examined histologically so far. BNC are normally fairly evenly distributed throughout the fetal villus and all their granules contain a similar variety of hormones and pregnancy associated glycoproteins (PAGs). Only the Giraffe is reported to show a different BNC protein expression, this paper is designed to investigate that. Gold labelled Lectin histochemistry and protein immunocytochemistry were used on deplasticised 1 μm sections of a wide variety of ruminant placentomes with a wide range of antibodies and lectins. In the Giraffe placentomes, even though the lectin histochemistry shows an even distribution of BNC throughout the trophoblast of the placental villi, the protein expression in the BNC granules is limited to the BNC either in the apex or the base of the villi. Placental lactogens and Prolactin (PRL) are present only in basally situated BNC: PAGs only in the apical BNC. PRL is only found in the Giraffe BNC which react with many fewer of the wide range of antibodies used here to investigate the uniformity of protein expression in ruminant BNC. The possible relevance of these differences to ruminant function and evolution is considered to provide a further example of the versatility of the BNC system. Oxford University Press 2022-01-17 /pmc/articles/PMC9305501/ /pubmed/35040958 http://dx.doi.org/10.1093/biolre/ioab247 Text en © The Author(s) 2022. Published by Oxford University Press behalf of Society for the Study of Reproduction. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wooding, F B P Forhead, A J Wilsher, S Allen, W R Roberts, R M Green, J A Beckers, J F Sousa, N Melo Charpigny, G Asymmetric expression of proteins in the granules of the placentomal Binucleate cells in Giraffa camelopardalis |
title | Asymmetric expression of proteins in the granules of the placentomal Binucleate cells in Giraffa camelopardalis |
title_full | Asymmetric expression of proteins in the granules of the placentomal Binucleate cells in Giraffa camelopardalis |
title_fullStr | Asymmetric expression of proteins in the granules of the placentomal Binucleate cells in Giraffa camelopardalis |
title_full_unstemmed | Asymmetric expression of proteins in the granules of the placentomal Binucleate cells in Giraffa camelopardalis |
title_short | Asymmetric expression of proteins in the granules of the placentomal Binucleate cells in Giraffa camelopardalis |
title_sort | asymmetric expression of proteins in the granules of the placentomal binucleate cells in giraffa camelopardalis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305501/ https://www.ncbi.nlm.nih.gov/pubmed/35040958 http://dx.doi.org/10.1093/biolre/ioab247 |
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