Cargando…

Asymmetric expression of proteins in the granules of the placentomal Binucleate cells in Giraffa camelopardalis

Mature granulated trophoblast binucleate cells (BNC) have been found in all ruminant placentas examined histologically so far. BNC are normally fairly evenly distributed throughout the fetal villus and all their granules contain a similar variety of hormones and pregnancy associated glycoproteins (P...

Descripción completa

Detalles Bibliográficos
Autores principales: Wooding, F B P, Forhead, A J, Wilsher, S, Allen, W R, Roberts, R M, Green, J A, Beckers, J F, Sousa, N Melo, Charpigny, G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305501/
https://www.ncbi.nlm.nih.gov/pubmed/35040958
http://dx.doi.org/10.1093/biolre/ioab247
_version_ 1784752341162917888
author Wooding, F B P
Forhead, A J
Wilsher, S
Allen, W R
Roberts, R M
Green, J A
Beckers, J F
Sousa, N Melo
Charpigny, G
author_facet Wooding, F B P
Forhead, A J
Wilsher, S
Allen, W R
Roberts, R M
Green, J A
Beckers, J F
Sousa, N Melo
Charpigny, G
author_sort Wooding, F B P
collection PubMed
description Mature granulated trophoblast binucleate cells (BNC) have been found in all ruminant placentas examined histologically so far. BNC are normally fairly evenly distributed throughout the fetal villus and all their granules contain a similar variety of hormones and pregnancy associated glycoproteins (PAGs). Only the Giraffe is reported to show a different BNC protein expression, this paper is designed to investigate that. Gold labelled Lectin histochemistry and protein immunocytochemistry were used on deplasticised 1 μm sections of a wide variety of ruminant placentomes with a wide range of antibodies and lectins. In the Giraffe placentomes, even though the lectin histochemistry shows an even distribution of BNC throughout the trophoblast of the placental villi, the protein expression in the BNC granules is limited to the BNC either in the apex or the base of the villi. Placental lactogens and Prolactin (PRL) are present only in basally situated BNC: PAGs only in the apical BNC. PRL is only found in the Giraffe BNC which react with many fewer of the wide range of antibodies used here to investigate the uniformity of protein expression in ruminant BNC. The possible relevance of these differences to ruminant function and evolution is considered to provide a further example of the versatility of the BNC system.
format Online
Article
Text
id pubmed-9305501
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-93055012022-07-22 Asymmetric expression of proteins in the granules of the placentomal Binucleate cells in Giraffa camelopardalis Wooding, F B P Forhead, A J Wilsher, S Allen, W R Roberts, R M Green, J A Beckers, J F Sousa, N Melo Charpigny, G Biol Reprod Research Article Mature granulated trophoblast binucleate cells (BNC) have been found in all ruminant placentas examined histologically so far. BNC are normally fairly evenly distributed throughout the fetal villus and all their granules contain a similar variety of hormones and pregnancy associated glycoproteins (PAGs). Only the Giraffe is reported to show a different BNC protein expression, this paper is designed to investigate that. Gold labelled Lectin histochemistry and protein immunocytochemistry were used on deplasticised 1 μm sections of a wide variety of ruminant placentomes with a wide range of antibodies and lectins. In the Giraffe placentomes, even though the lectin histochemistry shows an even distribution of BNC throughout the trophoblast of the placental villi, the protein expression in the BNC granules is limited to the BNC either in the apex or the base of the villi. Placental lactogens and Prolactin (PRL) are present only in basally situated BNC: PAGs only in the apical BNC. PRL is only found in the Giraffe BNC which react with many fewer of the wide range of antibodies used here to investigate the uniformity of protein expression in ruminant BNC. The possible relevance of these differences to ruminant function and evolution is considered to provide a further example of the versatility of the BNC system. Oxford University Press 2022-01-17 /pmc/articles/PMC9305501/ /pubmed/35040958 http://dx.doi.org/10.1093/biolre/ioab247 Text en © The Author(s) 2022. Published by Oxford University Press behalf of Society for the Study of Reproduction. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wooding, F B P
Forhead, A J
Wilsher, S
Allen, W R
Roberts, R M
Green, J A
Beckers, J F
Sousa, N Melo
Charpigny, G
Asymmetric expression of proteins in the granules of the placentomal Binucleate cells in Giraffa camelopardalis
title Asymmetric expression of proteins in the granules of the placentomal Binucleate cells in Giraffa camelopardalis
title_full Asymmetric expression of proteins in the granules of the placentomal Binucleate cells in Giraffa camelopardalis
title_fullStr Asymmetric expression of proteins in the granules of the placentomal Binucleate cells in Giraffa camelopardalis
title_full_unstemmed Asymmetric expression of proteins in the granules of the placentomal Binucleate cells in Giraffa camelopardalis
title_short Asymmetric expression of proteins in the granules of the placentomal Binucleate cells in Giraffa camelopardalis
title_sort asymmetric expression of proteins in the granules of the placentomal binucleate cells in giraffa camelopardalis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305501/
https://www.ncbi.nlm.nih.gov/pubmed/35040958
http://dx.doi.org/10.1093/biolre/ioab247
work_keys_str_mv AT woodingfbp asymmetricexpressionofproteinsinthegranulesoftheplacentomalbinucleatecellsingiraffacamelopardalis
AT forheadaj asymmetricexpressionofproteinsinthegranulesoftheplacentomalbinucleatecellsingiraffacamelopardalis
AT wilshers asymmetricexpressionofproteinsinthegranulesoftheplacentomalbinucleatecellsingiraffacamelopardalis
AT allenwr asymmetricexpressionofproteinsinthegranulesoftheplacentomalbinucleatecellsingiraffacamelopardalis
AT robertsrm asymmetricexpressionofproteinsinthegranulesoftheplacentomalbinucleatecellsingiraffacamelopardalis
AT greenja asymmetricexpressionofproteinsinthegranulesoftheplacentomalbinucleatecellsingiraffacamelopardalis
AT beckersjf asymmetricexpressionofproteinsinthegranulesoftheplacentomalbinucleatecellsingiraffacamelopardalis
AT sousanmelo asymmetricexpressionofproteinsinthegranulesoftheplacentomalbinucleatecellsingiraffacamelopardalis
AT charpignyg asymmetricexpressionofproteinsinthegranulesoftheplacentomalbinucleatecellsingiraffacamelopardalis