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Controlled Induction of Parthenogenesis in Transgenic Rice via Post-translational Activation of PsASGR-BBML

Modern plant breeding programs rely heavily on the generation of homozygous lines, with the traditional process requiring the inbreeding of a heterozygous cross for five to six generations. Doubled haploid (DH) technology, a process of generating haploid plants from an initial heterozygote, followed...

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Autores principales: Sidhu, Gurjot Singh, Conner, Joann A., Ozias-Akins, Peggy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305695/
https://www.ncbi.nlm.nih.gov/pubmed/35873991
http://dx.doi.org/10.3389/fpls.2022.925467
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author Sidhu, Gurjot Singh
Conner, Joann A.
Ozias-Akins, Peggy
author_facet Sidhu, Gurjot Singh
Conner, Joann A.
Ozias-Akins, Peggy
author_sort Sidhu, Gurjot Singh
collection PubMed
description Modern plant breeding programs rely heavily on the generation of homozygous lines, with the traditional process requiring the inbreeding of a heterozygous cross for five to six generations. Doubled haploid (DH) technology, a process of generating haploid plants from an initial heterozygote, followed by chromosome doubling, reduces the process to two generations. Currently established in vitro methods of haploid induction include androgenesis and gynogenesis, while in vivo methods are based on uni-parental genome elimination. Parthenogenesis, embryogenesis from unfertilized egg cells, presents another potential method of haploid induction. PsASGR-BABY BOOM-like, an AP2 transcription factor, induces parthenogenesis in a natural apomictic species, Pennisetum squamulatum (Cenchrus squamulatus) and PsASGR-BBML transgenes promote parthenogenesis in several crop plants, including rice, maize, and pearl millet. The dominant nature of PsASGR-BBML transgenes impedes their use in DH technology. Using a glucocorticoid-based post-translational regulation system and watering with a 100 μM DEX solution before anthesis, PsASGR-BBML can be regulated at the flowering stage to promote parthenogenesis. Conditional expression presents a novel opportunity to use parthenogenetic genes in DH production technology and to elucidate the molecular mechanism underlying parthenogenetic embryogenesis.
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spelling pubmed-93056952022-07-23 Controlled Induction of Parthenogenesis in Transgenic Rice via Post-translational Activation of PsASGR-BBML Sidhu, Gurjot Singh Conner, Joann A. Ozias-Akins, Peggy Front Plant Sci Plant Science Modern plant breeding programs rely heavily on the generation of homozygous lines, with the traditional process requiring the inbreeding of a heterozygous cross for five to six generations. Doubled haploid (DH) technology, a process of generating haploid plants from an initial heterozygote, followed by chromosome doubling, reduces the process to two generations. Currently established in vitro methods of haploid induction include androgenesis and gynogenesis, while in vivo methods are based on uni-parental genome elimination. Parthenogenesis, embryogenesis from unfertilized egg cells, presents another potential method of haploid induction. PsASGR-BABY BOOM-like, an AP2 transcription factor, induces parthenogenesis in a natural apomictic species, Pennisetum squamulatum (Cenchrus squamulatus) and PsASGR-BBML transgenes promote parthenogenesis in several crop plants, including rice, maize, and pearl millet. The dominant nature of PsASGR-BBML transgenes impedes their use in DH technology. Using a glucocorticoid-based post-translational regulation system and watering with a 100 μM DEX solution before anthesis, PsASGR-BBML can be regulated at the flowering stage to promote parthenogenesis. Conditional expression presents a novel opportunity to use parthenogenetic genes in DH production technology and to elucidate the molecular mechanism underlying parthenogenetic embryogenesis. Frontiers Media S.A. 2022-07-08 /pmc/articles/PMC9305695/ /pubmed/35873991 http://dx.doi.org/10.3389/fpls.2022.925467 Text en Copyright © 2022 Sidhu, Conner and Ozias-Akins. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Sidhu, Gurjot Singh
Conner, Joann A.
Ozias-Akins, Peggy
Controlled Induction of Parthenogenesis in Transgenic Rice via Post-translational Activation of PsASGR-BBML
title Controlled Induction of Parthenogenesis in Transgenic Rice via Post-translational Activation of PsASGR-BBML
title_full Controlled Induction of Parthenogenesis in Transgenic Rice via Post-translational Activation of PsASGR-BBML
title_fullStr Controlled Induction of Parthenogenesis in Transgenic Rice via Post-translational Activation of PsASGR-BBML
title_full_unstemmed Controlled Induction of Parthenogenesis in Transgenic Rice via Post-translational Activation of PsASGR-BBML
title_short Controlled Induction of Parthenogenesis in Transgenic Rice via Post-translational Activation of PsASGR-BBML
title_sort controlled induction of parthenogenesis in transgenic rice via post-translational activation of psasgr-bbml
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305695/
https://www.ncbi.nlm.nih.gov/pubmed/35873991
http://dx.doi.org/10.3389/fpls.2022.925467
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