Single-particle detection of native SARS-CoV-2 virions by microfluidic resistive pulse sensing

Microfluidic resistive pulse sensing (MRPS) was used to determine the size –distribution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) based on detecting nearly 30,000 single virions. However, the ultrastructure of SARS-CoV-2 is thoroughly described, but ensemble properties of SARS...

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Autores principales: Varga, Zoltán, Madai, Mónika, Kemenesi, Gábor, Beke-Somfai, Tamás, Jakab, Ferenc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier B.V. 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9306222/
https://www.ncbi.nlm.nih.gov/pubmed/35907357
http://dx.doi.org/10.1016/j.colsurfb.2022.112716
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author Varga, Zoltán
Madai, Mónika
Kemenesi, Gábor
Beke-Somfai, Tamás
Jakab, Ferenc
author_facet Varga, Zoltán
Madai, Mónika
Kemenesi, Gábor
Beke-Somfai, Tamás
Jakab, Ferenc
author_sort Varga, Zoltán
collection PubMed
description Microfluidic resistive pulse sensing (MRPS) was used to determine the size –distribution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) based on detecting nearly 30,000 single virions. However, the ultrastructure of SARS-CoV-2 is thoroughly described, but ensemble properties of SARS-CoV-2, e.g., its particle size distribution, are sparsely reported. According to the MRPS results, the size distribution of SARS-CoV-2 follows a log-normal function with a mean value of 85.1 nm, which corresponds to an approximate diameter of the viral envelope. This result also confirms the low number (< 50) of spike proteins on the surface of the virions.
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spelling pubmed-93062222022-07-22 Single-particle detection of native SARS-CoV-2 virions by microfluidic resistive pulse sensing Varga, Zoltán Madai, Mónika Kemenesi, Gábor Beke-Somfai, Tamás Jakab, Ferenc Colloids Surf B Biointerfaces Article Microfluidic resistive pulse sensing (MRPS) was used to determine the size –distribution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) based on detecting nearly 30,000 single virions. However, the ultrastructure of SARS-CoV-2 is thoroughly described, but ensemble properties of SARS-CoV-2, e.g., its particle size distribution, are sparsely reported. According to the MRPS results, the size distribution of SARS-CoV-2 follows a log-normal function with a mean value of 85.1 nm, which corresponds to an approximate diameter of the viral envelope. This result also confirms the low number (< 50) of spike proteins on the surface of the virions. The Authors. Published by Elsevier B.V. 2022-10 2022-07-22 /pmc/articles/PMC9306222/ /pubmed/35907357 http://dx.doi.org/10.1016/j.colsurfb.2022.112716 Text en © 2022 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Varga, Zoltán
Madai, Mónika
Kemenesi, Gábor
Beke-Somfai, Tamás
Jakab, Ferenc
Single-particle detection of native SARS-CoV-2 virions by microfluidic resistive pulse sensing
title Single-particle detection of native SARS-CoV-2 virions by microfluidic resistive pulse sensing
title_full Single-particle detection of native SARS-CoV-2 virions by microfluidic resistive pulse sensing
title_fullStr Single-particle detection of native SARS-CoV-2 virions by microfluidic resistive pulse sensing
title_full_unstemmed Single-particle detection of native SARS-CoV-2 virions by microfluidic resistive pulse sensing
title_short Single-particle detection of native SARS-CoV-2 virions by microfluidic resistive pulse sensing
title_sort single-particle detection of native sars-cov-2 virions by microfluidic resistive pulse sensing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9306222/
https://www.ncbi.nlm.nih.gov/pubmed/35907357
http://dx.doi.org/10.1016/j.colsurfb.2022.112716
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