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Dependence on clade II bHLH transcription factors for nursing of haploid products by tapetal‐like cells is conserved between moss sporangia and angiosperm anthers
Clade II basic helix–loop–helix transcription factors (bHLH TFs) are essential for pollen production and tapetal nursing functions in angiosperm anthers. As pollen has been suggested to be related to bryophyte spores by descent, we characterized two Physcomitrium (Physcomitrella) patens clade II bHL...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9306660/ https://www.ncbi.nlm.nih.gov/pubmed/35037245 http://dx.doi.org/10.1111/nph.17972 |
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author | Lopez‐Obando, Mauricio Landberg, Katarina Sundberg, Eva Thelander, Mattias |
author_facet | Lopez‐Obando, Mauricio Landberg, Katarina Sundberg, Eva Thelander, Mattias |
author_sort | Lopez‐Obando, Mauricio |
collection | PubMed |
description | Clade II basic helix–loop–helix transcription factors (bHLH TFs) are essential for pollen production and tapetal nursing functions in angiosperm anthers. As pollen has been suggested to be related to bryophyte spores by descent, we characterized two Physcomitrium (Physcomitrella) patens clade II bHLH TFs (PpbHLH092 and PpbHLH098), to test if regulation of sporogenous cells and the nursing cells surrounding them is conserved between angiosperm anthers and bryophyte sporangia. We made CRISPR‐Cas9 reporter and loss‐of‐function lines to address the function of PpbHLH092/098. We sectioned and analyzed WT and mutant sporophytes for a comprehensive stage‐by‐stage comparison of sporangium development. Spore precursors in the P. patens sporangium are surrounded by nursing cells showing striking similarities to tapetal cells in angiosperms. Moss clade II bHLH TFs are essential for the differentiation of these tapetal‐like cells and for the production of functional spores. Clade II bHLH TFs provide a conserved role in controlling the sporophytic somatic cells surrounding and nursing the sporogenous cells in both moss sporangia and angiosperm anthers. This supports the hypothesis that such nursing functions in mosses and angiosperms, lineages separated by c. 450 million years, are related by descent. |
format | Online Article Text |
id | pubmed-9306660 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93066602022-07-28 Dependence on clade II bHLH transcription factors for nursing of haploid products by tapetal‐like cells is conserved between moss sporangia and angiosperm anthers Lopez‐Obando, Mauricio Landberg, Katarina Sundberg, Eva Thelander, Mattias New Phytol Research Clade II basic helix–loop–helix transcription factors (bHLH TFs) are essential for pollen production and tapetal nursing functions in angiosperm anthers. As pollen has been suggested to be related to bryophyte spores by descent, we characterized two Physcomitrium (Physcomitrella) patens clade II bHLH TFs (PpbHLH092 and PpbHLH098), to test if regulation of sporogenous cells and the nursing cells surrounding them is conserved between angiosperm anthers and bryophyte sporangia. We made CRISPR‐Cas9 reporter and loss‐of‐function lines to address the function of PpbHLH092/098. We sectioned and analyzed WT and mutant sporophytes for a comprehensive stage‐by‐stage comparison of sporangium development. Spore precursors in the P. patens sporangium are surrounded by nursing cells showing striking similarities to tapetal cells in angiosperms. Moss clade II bHLH TFs are essential for the differentiation of these tapetal‐like cells and for the production of functional spores. Clade II bHLH TFs provide a conserved role in controlling the sporophytic somatic cells surrounding and nursing the sporogenous cells in both moss sporangia and angiosperm anthers. This supports the hypothesis that such nursing functions in mosses and angiosperms, lineages separated by c. 450 million years, are related by descent. John Wiley and Sons Inc. 2022-02-05 2022-07 /pmc/articles/PMC9306660/ /pubmed/35037245 http://dx.doi.org/10.1111/nph.17972 Text en © 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Lopez‐Obando, Mauricio Landberg, Katarina Sundberg, Eva Thelander, Mattias Dependence on clade II bHLH transcription factors for nursing of haploid products by tapetal‐like cells is conserved between moss sporangia and angiosperm anthers |
title | Dependence on clade II bHLH transcription factors for nursing of haploid products by tapetal‐like cells is conserved between moss sporangia and angiosperm anthers |
title_full | Dependence on clade II bHLH transcription factors for nursing of haploid products by tapetal‐like cells is conserved between moss sporangia and angiosperm anthers |
title_fullStr | Dependence on clade II bHLH transcription factors for nursing of haploid products by tapetal‐like cells is conserved between moss sporangia and angiosperm anthers |
title_full_unstemmed | Dependence on clade II bHLH transcription factors for nursing of haploid products by tapetal‐like cells is conserved between moss sporangia and angiosperm anthers |
title_short | Dependence on clade II bHLH transcription factors for nursing of haploid products by tapetal‐like cells is conserved between moss sporangia and angiosperm anthers |
title_sort | dependence on clade ii bhlh transcription factors for nursing of haploid products by tapetal‐like cells is conserved between moss sporangia and angiosperm anthers |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9306660/ https://www.ncbi.nlm.nih.gov/pubmed/35037245 http://dx.doi.org/10.1111/nph.17972 |
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