Blind deconvolution in autocorrelation inversion for multiview light‐sheet microscopy

Combining the information coming from multiview acquisitions is a problem of great interest in light‐sheet microscopy. Aligning the views and increasing the resolution of their fusion can be challenging, especially if the setup is not fully calibrated. Here, we tackle these issues by proposing a new reconstruction method based on autocorrelation inversion that avoids alignment procedures. On top of this, we add a blind deconvolution step to improve the resolution of the final reconstruction. Our method permits us to achieve inherently aligned, highly resolved reconstructions while, at the same time, estimating the unknown point‐spread function of the system. RESEARCH HIGHLIGHTS: We tackle the problem of multiview light‐sheet deconvolution with a blind approach of autocorrelation inversion. Our method recovers the object and PSF, requires no alignment and calibration, and enhances the reconstruction of the specimen.