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Detection methods targeting the positive‐ and negative‐sense RNA transcripts from plus‐stranded RNA viruses
The largest group of viruses in the Baltimore classification system comprises viruses with a positive‐sense, single‐stranded RNA genome. Once the viral genome is released into the cytoplasm of a specific host cell following virus entry, it functions directly as an mRNA, and the virus‐encoded protein...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9306919/ https://www.ncbi.nlm.nih.gov/pubmed/34939239 http://dx.doi.org/10.1111/apm.13202 |
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author | Warncke, Signe Ravn Knudsen, Charlotte Rohde |
author_facet | Warncke, Signe Ravn Knudsen, Charlotte Rohde |
author_sort | Warncke, Signe Ravn |
collection | PubMed |
description | The largest group of viruses in the Baltimore classification system comprises viruses with a positive‐sense, single‐stranded RNA genome. Once the viral genome is released into the cytoplasm of a specific host cell following virus entry, it functions directly as an mRNA, and the virus‐encoded proteins that are essential for genome replication are produced by the translation apparatus of the host cell. The positive‐sense genome is replicated in two stages, initially the positive strand is copied to make a negative‐sense RNA, which then functions as the template for transcription of many new positive‐sense genomes. Virus infections can be detected at different stages throughout the infection cycle for diagnostic and scientific purposes. Here, the advantages and disadvantages of some of the relevant methods for genome detection will be briefly reviewed with special emphasis on techniques allowing strand‐specific RNA detection. Furthermore, tools of the future are considered. |
format | Online Article Text |
id | pubmed-9306919 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93069192022-07-28 Detection methods targeting the positive‐ and negative‐sense RNA transcripts from plus‐stranded RNA viruses Warncke, Signe Ravn Knudsen, Charlotte Rohde APMIS Original Articles The largest group of viruses in the Baltimore classification system comprises viruses with a positive‐sense, single‐stranded RNA genome. Once the viral genome is released into the cytoplasm of a specific host cell following virus entry, it functions directly as an mRNA, and the virus‐encoded proteins that are essential for genome replication are produced by the translation apparatus of the host cell. The positive‐sense genome is replicated in two stages, initially the positive strand is copied to make a negative‐sense RNA, which then functions as the template for transcription of many new positive‐sense genomes. Virus infections can be detected at different stages throughout the infection cycle for diagnostic and scientific purposes. Here, the advantages and disadvantages of some of the relevant methods for genome detection will be briefly reviewed with special emphasis on techniques allowing strand‐specific RNA detection. Furthermore, tools of the future are considered. John Wiley and Sons Inc. 2022-01-26 2022-05 /pmc/articles/PMC9306919/ /pubmed/34939239 http://dx.doi.org/10.1111/apm.13202 Text en © 2021 The Authors. APMIS published by John Wiley & Sons Ltd on behalf of Scandinavian Societies for Medical Microbiology and Pathology. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Warncke, Signe Ravn Knudsen, Charlotte Rohde Detection methods targeting the positive‐ and negative‐sense RNA transcripts from plus‐stranded RNA viruses |
title | Detection methods targeting the positive‐ and negative‐sense RNA transcripts from plus‐stranded RNA viruses |
title_full | Detection methods targeting the positive‐ and negative‐sense RNA transcripts from plus‐stranded RNA viruses |
title_fullStr | Detection methods targeting the positive‐ and negative‐sense RNA transcripts from plus‐stranded RNA viruses |
title_full_unstemmed | Detection methods targeting the positive‐ and negative‐sense RNA transcripts from plus‐stranded RNA viruses |
title_short | Detection methods targeting the positive‐ and negative‐sense RNA transcripts from plus‐stranded RNA viruses |
title_sort | detection methods targeting the positive‐ and negative‐sense rna transcripts from plus‐stranded rna viruses |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9306919/ https://www.ncbi.nlm.nih.gov/pubmed/34939239 http://dx.doi.org/10.1111/apm.13202 |
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