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Alveolar crystal burden in stone workers with artificial stone silicosis

BACKGROUND AND OBJECTIVE: An epidemic of silicosis has emerged due to a failure to control risks associated with exposure to high‐silica content respirable dust generated while working with artificial stone products. Methods for quantification of alveolar crystal burden are needed to advance our und...

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Autores principales: Apte, Simon H., Tan, Maxine E., Lutzky, Viviana P., De Silva, Tharushi A., Fiene, Andreas, Hundloe, Justin, Deller, David, Sullivan, Clair, Bell, Peter T., Chambers, Daniel C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307012/
https://www.ncbi.nlm.nih.gov/pubmed/35176815
http://dx.doi.org/10.1111/resp.14229
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author Apte, Simon H.
Tan, Maxine E.
Lutzky, Viviana P.
De Silva, Tharushi A.
Fiene, Andreas
Hundloe, Justin
Deller, David
Sullivan, Clair
Bell, Peter T.
Chambers, Daniel C.
author_facet Apte, Simon H.
Tan, Maxine E.
Lutzky, Viviana P.
De Silva, Tharushi A.
Fiene, Andreas
Hundloe, Justin
Deller, David
Sullivan, Clair
Bell, Peter T.
Chambers, Daniel C.
author_sort Apte, Simon H.
collection PubMed
description BACKGROUND AND OBJECTIVE: An epidemic of silicosis has emerged due to a failure to control risks associated with exposure to high‐silica content respirable dust generated while working with artificial stone products. Methods for quantification of alveolar crystal burden are needed to advance our understanding of the pathobiology of silica‐related lung injury as well as assisting in the diagnosis, clinical management and prognostication of affected workers. The objective of this study was to develop and validate novel methods to quantify alveolar crystal burden in bronchoalveolar lavage (BAL) fluid from patients with artificial stone silicosis. METHODS: New methods to quantify and analyse alveolar crystal in BAL from patients with artificial stone silicosis were developed. Crystals were isolated and counted by microscopy and alveolar crystal burden was calculated using a standard curve generated by titration of respirable α‐Quartz. The utility of the assay was then assessed in 23 patients with artificial stone silicosis. RESULTS: Alveolar crystal burden was greater in patients with silicosis (0.44 picograms [pg]/cell [0.08–3.49]) compared to patients with other respiratory diagnoses (0.057 pg/cell [0.01–0.34]; p < 0.001). Alveolar crystal burden was positively correlated with years of silica exposure (ρ = 0.49, p = 0.02) and with decline in diffusing capacity of the lungs for carbon monoxide (ρ = −0.50, p = 0.02). CONCLUSION: Alveolar crystal burden quantification differentiates patients with silicosis from patients with other respiratory disorders. Furthermore, crystal burden is correlated with the rate of decline in lung function in patients with artificial stone silicosis.
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spelling pubmed-93070122022-07-28 Alveolar crystal burden in stone workers with artificial stone silicosis Apte, Simon H. Tan, Maxine E. Lutzky, Viviana P. De Silva, Tharushi A. Fiene, Andreas Hundloe, Justin Deller, David Sullivan, Clair Bell, Peter T. Chambers, Daniel C. Respirology Original Articles BACKGROUND AND OBJECTIVE: An epidemic of silicosis has emerged due to a failure to control risks associated with exposure to high‐silica content respirable dust generated while working with artificial stone products. Methods for quantification of alveolar crystal burden are needed to advance our understanding of the pathobiology of silica‐related lung injury as well as assisting in the diagnosis, clinical management and prognostication of affected workers. The objective of this study was to develop and validate novel methods to quantify alveolar crystal burden in bronchoalveolar lavage (BAL) fluid from patients with artificial stone silicosis. METHODS: New methods to quantify and analyse alveolar crystal in BAL from patients with artificial stone silicosis were developed. Crystals were isolated and counted by microscopy and alveolar crystal burden was calculated using a standard curve generated by titration of respirable α‐Quartz. The utility of the assay was then assessed in 23 patients with artificial stone silicosis. RESULTS: Alveolar crystal burden was greater in patients with silicosis (0.44 picograms [pg]/cell [0.08–3.49]) compared to patients with other respiratory diagnoses (0.057 pg/cell [0.01–0.34]; p < 0.001). Alveolar crystal burden was positively correlated with years of silica exposure (ρ = 0.49, p = 0.02) and with decline in diffusing capacity of the lungs for carbon monoxide (ρ = −0.50, p = 0.02). CONCLUSION: Alveolar crystal burden quantification differentiates patients with silicosis from patients with other respiratory disorders. Furthermore, crystal burden is correlated with the rate of decline in lung function in patients with artificial stone silicosis. John Wiley & Sons, Ltd 2022-02-17 2022-06 /pmc/articles/PMC9307012/ /pubmed/35176815 http://dx.doi.org/10.1111/resp.14229 Text en © 2022 The Authors. Respirology published by John Wiley & Sons Australia, Ltd on behalf of Asian Pacific Society of Respirology. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Apte, Simon H.
Tan, Maxine E.
Lutzky, Viviana P.
De Silva, Tharushi A.
Fiene, Andreas
Hundloe, Justin
Deller, David
Sullivan, Clair
Bell, Peter T.
Chambers, Daniel C.
Alveolar crystal burden in stone workers with artificial stone silicosis
title Alveolar crystal burden in stone workers with artificial stone silicosis
title_full Alveolar crystal burden in stone workers with artificial stone silicosis
title_fullStr Alveolar crystal burden in stone workers with artificial stone silicosis
title_full_unstemmed Alveolar crystal burden in stone workers with artificial stone silicosis
title_short Alveolar crystal burden in stone workers with artificial stone silicosis
title_sort alveolar crystal burden in stone workers with artificial stone silicosis
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307012/
https://www.ncbi.nlm.nih.gov/pubmed/35176815
http://dx.doi.org/10.1111/resp.14229
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