Cargando…

Immobilization of the Aspartate Ammonia‐Lyase from Pseudomonas fluorescens R124 on Magnetic Nanoparticles: Characterization and Kinetics

Aspartate ammonia‐lyases (AALs) catalyze the non‐oxidative elimination of ammonia from l‐aspartate to give fumarate and ammonia. In this work the AAL coding gene from Pseudomonas fluorescens R124 was identified, isolated, and cloned into the pET‐15b expression vector and expressed in E. coli. The pu...

Descripción completa

Detalles Bibliográficos
Autores principales:	Csuka, Pál, Molnár, Zsófia, Tóth, Veronika, Imarah, Ali Obaid, Balogh‐Weiser, Diána, Vértessy, Beáta G., Poppe, László
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2022
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307013/ https://www.ncbi.nlm.nih.gov/pubmed/35114050 http://dx.doi.org/10.1002/cbic.202100708

Ejemplares similares

“Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media
por: Sánta-Bell, Evelin, et al.
Publicado: (2019)

Immobilization of Phenylalanine Ammonia‐Lyase on Single‐Walled Carbon Nanotubes for Stereoselective Biotransformations in Batch and Continuous‐Flow Modes
por: Bartha‐Vári, Judith H., et al.
Publicado: (2015)

A Methylidene Group in the Phosphonic Acid Analogue of Phenylalanine Reverses the Enantiopreference of Binding to Phenylalanine Ammonia‐Lyases
por: Bata, Zsófia, et al.
Publicado: (2017)

Expression and Properties of the Highly Alkalophilic Phenylalanine Ammonia-Lyase of Thermophilic Rubrobacter xylanophilus
por: Kovács, Klaudia, et al.
Publicado: (2014)

Role of aspartate ammonia-lyase in Pasteurella multocida
por: Wang, Zui, et al.
Publicado: (2020)

Robust, site-specifically immobilized phenylalanine ammonia-lyases for the enantioselective ammonia addition of cinnamic acids
por: Boros, Krisztina, et al.
Publicado: (2021)

Immobilization of Cross-Linked Phenylalanine Ammonia Lyase Aggregates in Microporous Silica Gel
por: Cui, Jian Dong, et al.
Publicado: (2013)

Smart Nanoparticles for Selective Immobilization of Acid Phosphatases
por: Nagy, Flóra, et al.
Publicado: (2018)

Immobilization of Lipase B from Candida antarctica on Magnetic Nanoparticles Enhances Its Selectivity in Kinetic Resolutions of Chiral Amines with Several Acylating Agents
por: Silva, Fausto M. W. G., et al.
Publicado: (2023)

Tailored Mutants of Phenylalanine Ammonia‐Lyase from Petroselinum crispum for the Synthesis of Bulky l‐ and d‐Arylalanines
por: Filip, Alina, et al.
Publicado: (2018)

Green synthesis and in situ immobilization of gold nanoparticles and their application for the reduction of p-nitrophenol in continuous-flow mode
por: Szűcs, Rózsa, et al.
Publicado: (2019)

Fungal and Plant Phenylalanine Ammonia-lyase
por: Hyun, Min Woo, et al.
Publicado: (2011)

The production of l- and d-phenylalanines using engineered phenylalanine ammonia lyases from Petroselinum crispum
por: Tork, Souad Diana, et al.
Publicado: (2019)

Saturation Mutagenesis for Phenylalanine Ammonia Lyases of Enhanced Catalytic Properties
por: Tomoiagă, Raluca Bianca, et al.
Publicado: (2020)

Fluorescent enzyme-coupled activity assay for phenylalanine ammonia-lyases
por: Moisă, Mădălina E., et al.
Publicado: (2020)

Isocitrate Lyase and Succinate Semialdehyde Dehydrogenase Mediate the Synthesis of α-Ketoglutarate in Pseudomonas fluorescens
por: Alhasawi, Azhar A., et al.
Publicado: (2019)

Genomic Characterization of Phenylalanine Ammonia Lyase Gene in Buckwheat
por: Thiyagarajan, Karthikeyan, et al.
Publicado: (2016)

Towards a general approach for tailoring the hydrophobic binding site of phenylalanine ammonia-lyases
por: Tork, Souad Diana, et al.
Publicado: (2022)

Purification and Characterization of Phenylalanine Ammonia Lyase from Trichosporon cutaneum
por: Goldson-Barnaby, Andrea, et al.
Publicado: (2013)

Production of Trans-Cinnamic Acid by Immobilization of the Bambusa oldhamii BoPAL1 and BoPAL2 Phenylalanine Ammonia-Lyases on Electrospun Nanofibers
por: Hong, Pei-Yu, et al.
Publicado: (2021)

Expression of phenylalanine ammonia lyase as an intracellularly free and extracellularly cell surface-immobilized enzyme on a gut microbe as a live biotherapeutic for phenylketonuria
por: Jiang, Yu, et al.
Publicado: (2022)

Immobilization of BoPAL3 Phenylalanine Ammonia-Lyase on Electrospun Nanofibrous Membranes of Polyvinyl Alcohol/Nylon 6/Chitosan Crosslinked with Dextran Polyaldehyde
por: Hsieh, Chun-Yen, et al.
Publicado: (2023)

Characterization of a thermostable methylaspartate ammonia lyase from Carboxydothermus hydrogenoformans
por: Raj, Hans, et al.
Publicado: (2011)

The roles of active site residues in the catalytic mechanism of methylaspartate ammonia-lyase()
por: Raj, Hans, et al.
Publicado: (2013)

Zymophore identification enables the discovery of novel phenylalanine ammonia lyase enzymes
por: Weise, Nicholas J., et al.
Publicado: (2017)

Effects of Enzyme Loading and Immobilization Conditions on the Catalytic Features of Lipase From Pseudomonas fluorescens Immobilized on Octyl-Agarose Beads
por: Arana-Peña, Sara, et al.
Publicado: (2020)

Insights from the analysis of alginate lyase protein model from Pseudomonas fluorescens towards the understanding of mucoid biofilm disruption
por: Singh, Gurjant, et al.
Publicado: (2017)

Formulation and PEGylation optimization of the therapeutic PEGylated phenylalanine ammonia lyase for the treatment of phenylketonuria
por: Bell, Sean M., et al.
Publicado: (2017)

Microreactor equipped with naturally acid-resistant histidine ammonia lyase from an extremophile
por: Ade, Carina, et al.
Publicado: (2022)

Discovery of Novel Tyrosine Ammonia Lyases for the Enzymatic Synthesis of p‐Coumaric Acid
por: Brack, Yannik, et al.
Publicado: (2022)

Functional and kinetics of two efficient phenylalanine ammonia lyase from Pyrus bretschneideri
por: Li, Guohui, et al.
Publicado: (2023)

Transaminase-mediated synthesis of enantiopure drug-like 1-(3′,4′-disubstituted phenyl)propan-2-amines
por: Lakó, Ágnes, et al.
Publicado: (2020)

The phenylalanine ammonia lyase (PAL) gene family shows a gymnosperm-specific lineage
por: Bagal, Ujwal R, et al.
Publicado: (2012)

Molecular Cloning and Sequence Analysis of a Phenylalanine Ammonia-Lyase Gene from Dendrobium
por: Jin, Qing, et al.
Publicado: (2013)

Metabolism of β-valine via a CoA-dependent ammonia lyase pathway
por: Otzen, Marleen, et al.
Publicado: (2015)

Molecular Cloning, Characterization and Expression of the Phenylalanine Ammonia-Lyase Gene from Juglans regia
por: Xu, Feng, et al.
Publicado: (2012)

Improving the Catalytic Performance of Pectate Lyase Through Pectate Lyase/Cu(3)(PO(4))(2) Hybrid Nanoflowers as an Immobilized Enzyme
por: Wu, Pan, et al.
Publicado: (2020)

Efficient Preparation of Enantiopure D-Phenylalanine through Asymmetric Resolution Using Immobilized Phenylalanine Ammonia-Lyase from Rhodotorula glutinis JN-1 in a Recirculating Packed-Bed Reactor
por: Zhu, Longbao, et al.
Publicado: (2014)

Synthesis of d- and l-Phenylalanine Derivatives by Phenylalanine Ammonia Lyases: A Multienzymatic Cascade Process**
por: Parmeggiani, Fabio, et al.
Publicado: (2015)

Synthesis of d‐ and l‐Phenylalanine Derivatives by Phenylalanine Ammonia Lyases: A Multienzymatic Cascade Process
por: Parmeggiani, Fabio, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_obg5ojtjghs5hc4odhqlidd31k