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Targeting early proximal-rod component substrate FlgB to FlhB for flagellar-type III secretion in Salmonella

The Salmonella flagellar secretion apparatus is a member of the type III secretion (T3S) family of export systems in bacteria. After completion of the flagellar motor structure, the hook-basal body (HBB), the flagellar T3S system undergoes a switch from early to late substrate secretion, which resul...

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Autores principales: Qu, Daofeng, Jiang, Mengxue, Duffin, Calder, Hughes, Kelly T., Chevance, Fabienne F. V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307174/
https://www.ncbi.nlm.nih.gov/pubmed/35819991
http://dx.doi.org/10.1371/journal.pgen.1010313
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author Qu, Daofeng
Jiang, Mengxue
Duffin, Calder
Hughes, Kelly T.
Chevance, Fabienne F. V.
author_facet Qu, Daofeng
Jiang, Mengxue
Duffin, Calder
Hughes, Kelly T.
Chevance, Fabienne F. V.
author_sort Qu, Daofeng
collection PubMed
description The Salmonella flagellar secretion apparatus is a member of the type III secretion (T3S) family of export systems in bacteria. After completion of the flagellar motor structure, the hook-basal body (HBB), the flagellar T3S system undergoes a switch from early to late substrate secretion, which results in the expression and assembly of the external, filament propeller-like structure. In order to characterize early substrate secretion-signals in the flagellar T3S system, the FlgB, and FlgC components of the flagellar rod, which acts as the drive-shaft within the HBB, were subject to deletion mutagenesis to identify regions of these proteins that were important for secretion. The β-lactamase protein lacking its Sec-dependent secretion signal (Bla) was fused to the C-terminus of FlgB and FlgC and used as a reporter to select for and quantify the secretion of FlgB and FlgC into the periplasm. Secretion of Bla into the periplasm confers resistance to ampicillin. In-frame deletions of amino acids 9 through 18 and amino acids 39 through 58 of FlgB decreased FlgB secretion levels while deleting amino acid 6 through 14 diminished FlgC secretion levels. Further PCR-directed mutagenesis indicated that amino acid F45 of FlgB was critical for secretion. Single amino acid mutagenesis revealed that all amino acid substitutions at F45 of FlgB position impaired rod assembly, which was due to a defect of FlgB secretion. An equivalent F49 position in FlgC was essential for assembly but not for secretion. This study also revealed that a hydrophobic patch in the cleaved C-terminal domain of FlhB is critical for recognition of FlgB at F45.
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spelling pubmed-93071742022-07-23 Targeting early proximal-rod component substrate FlgB to FlhB for flagellar-type III secretion in Salmonella Qu, Daofeng Jiang, Mengxue Duffin, Calder Hughes, Kelly T. Chevance, Fabienne F. V. PLoS Genet Research Article The Salmonella flagellar secretion apparatus is a member of the type III secretion (T3S) family of export systems in bacteria. After completion of the flagellar motor structure, the hook-basal body (HBB), the flagellar T3S system undergoes a switch from early to late substrate secretion, which results in the expression and assembly of the external, filament propeller-like structure. In order to characterize early substrate secretion-signals in the flagellar T3S system, the FlgB, and FlgC components of the flagellar rod, which acts as the drive-shaft within the HBB, were subject to deletion mutagenesis to identify regions of these proteins that were important for secretion. The β-lactamase protein lacking its Sec-dependent secretion signal (Bla) was fused to the C-terminus of FlgB and FlgC and used as a reporter to select for and quantify the secretion of FlgB and FlgC into the periplasm. Secretion of Bla into the periplasm confers resistance to ampicillin. In-frame deletions of amino acids 9 through 18 and amino acids 39 through 58 of FlgB decreased FlgB secretion levels while deleting amino acid 6 through 14 diminished FlgC secretion levels. Further PCR-directed mutagenesis indicated that amino acid F45 of FlgB was critical for secretion. Single amino acid mutagenesis revealed that all amino acid substitutions at F45 of FlgB position impaired rod assembly, which was due to a defect of FlgB secretion. An equivalent F49 position in FlgC was essential for assembly but not for secretion. This study also revealed that a hydrophobic patch in the cleaved C-terminal domain of FlhB is critical for recognition of FlgB at F45. Public Library of Science 2022-07-12 /pmc/articles/PMC9307174/ /pubmed/35819991 http://dx.doi.org/10.1371/journal.pgen.1010313 Text en © 2022 Qu et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Qu, Daofeng
Jiang, Mengxue
Duffin, Calder
Hughes, Kelly T.
Chevance, Fabienne F. V.
Targeting early proximal-rod component substrate FlgB to FlhB for flagellar-type III secretion in Salmonella
title Targeting early proximal-rod component substrate FlgB to FlhB for flagellar-type III secretion in Salmonella
title_full Targeting early proximal-rod component substrate FlgB to FlhB for flagellar-type III secretion in Salmonella
title_fullStr Targeting early proximal-rod component substrate FlgB to FlhB for flagellar-type III secretion in Salmonella
title_full_unstemmed Targeting early proximal-rod component substrate FlgB to FlhB for flagellar-type III secretion in Salmonella
title_short Targeting early proximal-rod component substrate FlgB to FlhB for flagellar-type III secretion in Salmonella
title_sort targeting early proximal-rod component substrate flgb to flhb for flagellar-type iii secretion in salmonella
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307174/
https://www.ncbi.nlm.nih.gov/pubmed/35819991
http://dx.doi.org/10.1371/journal.pgen.1010313
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