Potential pitfalls in analysing a SARS-CoV-2 RT-PCR assay and how to standardise data interpretation

The emergence of SARS-CoV-2 in December 2019 lead to the rapid implementation of assays for virus detection, with real-time RT-PCR arguably considered the gold-standard. In our laboratory Altona RealStar SARS-Cov-2 RT-PCR kits are used with Applied Biosystems QuantStudio 7 Flex thermocyclers. Real-t...

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Detalles Bibliográficos
Autores principales: Smith, Melvyn, El Bouzidi, Kate, Bengen, Simon, Cohen, Aron, Zuckerman, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307283/
https://www.ncbi.nlm.nih.gov/pubmed/35878653
http://dx.doi.org/10.1016/j.jviromet.2022.114589

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