Filter trapping protocol to detect aggregated proteins in human cell lines

The loss of protein homeostasis results in cytotoxic protein aggregates, a common hallmark of aging and neurological diseases. Here, we present an adjusted filter-trapping assay protocol to detect global aggregated proteins in human cell lines, via a high-sensitive protein staining method. This prot...

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Detalles Bibliográficos
Autores principales: Chhipi-Shrestha, Jagat K., Yoshida, Minoru, Iwasaki, Shintaro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307672/
https://www.ncbi.nlm.nih.gov/pubmed/35880124
http://dx.doi.org/10.1016/j.xpro.2022.101571
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author Chhipi-Shrestha, Jagat K.
Yoshida, Minoru
Iwasaki, Shintaro
author_facet Chhipi-Shrestha, Jagat K.
Yoshida, Minoru
Iwasaki, Shintaro
author_sort Chhipi-Shrestha, Jagat K.
collection PubMed
description The loss of protein homeostasis results in cytotoxic protein aggregates, a common hallmark of aging and neurological diseases. Here, we present an adjusted filter-trapping assay protocol to detect global aggregated proteins in human cell lines, via a high-sensitive protein staining method. This protocol also details an alternative approach to monitor specific protein aggregates trapped in the filter membrane, by subsequent immunoblotting of ectopically expressed and endogenous proteins. For complete details on the use and execution of this protocol, please refer to Chhipi-Shrestha et al. (2022).
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spelling pubmed-93076722022-07-24 Filter trapping protocol to detect aggregated proteins in human cell lines Chhipi-Shrestha, Jagat K. Yoshida, Minoru Iwasaki, Shintaro STAR Protoc Protocol The loss of protein homeostasis results in cytotoxic protein aggregates, a common hallmark of aging and neurological diseases. Here, we present an adjusted filter-trapping assay protocol to detect global aggregated proteins in human cell lines, via a high-sensitive protein staining method. This protocol also details an alternative approach to monitor specific protein aggregates trapped in the filter membrane, by subsequent immunoblotting of ectopically expressed and endogenous proteins. For complete details on the use and execution of this protocol, please refer to Chhipi-Shrestha et al. (2022). Elsevier 2022-07-19 /pmc/articles/PMC9307672/ /pubmed/35880124 http://dx.doi.org/10.1016/j.xpro.2022.101571 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Chhipi-Shrestha, Jagat K.
Yoshida, Minoru
Iwasaki, Shintaro
Filter trapping protocol to detect aggregated proteins in human cell lines
title Filter trapping protocol to detect aggregated proteins in human cell lines
title_full Filter trapping protocol to detect aggregated proteins in human cell lines
title_fullStr Filter trapping protocol to detect aggregated proteins in human cell lines
title_full_unstemmed Filter trapping protocol to detect aggregated proteins in human cell lines
title_short Filter trapping protocol to detect aggregated proteins in human cell lines
title_sort filter trapping protocol to detect aggregated proteins in human cell lines
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307672/
https://www.ncbi.nlm.nih.gov/pubmed/35880124
http://dx.doi.org/10.1016/j.xpro.2022.101571
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