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Filter trapping protocol to detect aggregated proteins in human cell lines

The loss of protein homeostasis results in cytotoxic protein aggregates, a common hallmark of aging and neurological diseases. Here, we present an adjusted filter-trapping assay protocol to detect global aggregated proteins in human cell lines, via a high-sensitive protein staining method. This prot...

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Detalles Bibliográficos
Autores principales: Chhipi-Shrestha, Jagat K., Yoshida, Minoru, Iwasaki, Shintaro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307672/
https://www.ncbi.nlm.nih.gov/pubmed/35880124
http://dx.doi.org/10.1016/j.xpro.2022.101571