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Nanoscale regulation of Ca(2+) dependent phase transitions and real-time dynamics of SAP97/hDLG

Synapse associated protein-97/Human Disk Large (SAP97/hDLG) is a conserved, alternatively spliced, modular, scaffolding protein critical in regulating the molecular organization of cell-cell junctions in vertebrates. We confirm that the molecular determinants of first order phase transition of SAP97...

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Autores principales: Rajeev, Premchand, Singh, Nivedita, Kechkar, Adel, Butler, Corey, Ramanan, Narendrakumar, Sibarita, Jean-Baptiste, Jose, Mini, Nair, Deepak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307800/
https://www.ncbi.nlm.nih.gov/pubmed/35869063
http://dx.doi.org/10.1038/s41467-022-31912-1
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author Rajeev, Premchand
Singh, Nivedita
Kechkar, Adel
Butler, Corey
Ramanan, Narendrakumar
Sibarita, Jean-Baptiste
Jose, Mini
Nair, Deepak
author_facet Rajeev, Premchand
Singh, Nivedita
Kechkar, Adel
Butler, Corey
Ramanan, Narendrakumar
Sibarita, Jean-Baptiste
Jose, Mini
Nair, Deepak
author_sort Rajeev, Premchand
collection PubMed
description Synapse associated protein-97/Human Disk Large (SAP97/hDLG) is a conserved, alternatively spliced, modular, scaffolding protein critical in regulating the molecular organization of cell-cell junctions in vertebrates. We confirm that the molecular determinants of first order phase transition of SAP97/hDLG is controlled by morpho-functional changes in its nanoscale organization. Furthermore, the nanoscale molecular signatures of these signalling islands and phase transitions are altered in response to changes in cytosolic Ca(2+). Additionally, exchange kinetics of alternatively spliced isoforms of the intrinsically disordered region in SAP97/hDLG C-terminus shows differential sensitivities to Ca(2+) bound Calmodulin, affirming that the molecular signatures of local phase transitions of SAP97/hDLG depends on their nanoscale heterogeneity and compositionality of isoforms.
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spelling pubmed-93078002022-07-24 Nanoscale regulation of Ca(2+) dependent phase transitions and real-time dynamics of SAP97/hDLG Rajeev, Premchand Singh, Nivedita Kechkar, Adel Butler, Corey Ramanan, Narendrakumar Sibarita, Jean-Baptiste Jose, Mini Nair, Deepak Nat Commun Article Synapse associated protein-97/Human Disk Large (SAP97/hDLG) is a conserved, alternatively spliced, modular, scaffolding protein critical in regulating the molecular organization of cell-cell junctions in vertebrates. We confirm that the molecular determinants of first order phase transition of SAP97/hDLG is controlled by morpho-functional changes in its nanoscale organization. Furthermore, the nanoscale molecular signatures of these signalling islands and phase transitions are altered in response to changes in cytosolic Ca(2+). Additionally, exchange kinetics of alternatively spliced isoforms of the intrinsically disordered region in SAP97/hDLG C-terminus shows differential sensitivities to Ca(2+) bound Calmodulin, affirming that the molecular signatures of local phase transitions of SAP97/hDLG depends on their nanoscale heterogeneity and compositionality of isoforms. Nature Publishing Group UK 2022-07-22 /pmc/articles/PMC9307800/ /pubmed/35869063 http://dx.doi.org/10.1038/s41467-022-31912-1 Text en © The Author(s) 2022, corrected publication 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Rajeev, Premchand
Singh, Nivedita
Kechkar, Adel
Butler, Corey
Ramanan, Narendrakumar
Sibarita, Jean-Baptiste
Jose, Mini
Nair, Deepak
Nanoscale regulation of Ca(2+) dependent phase transitions and real-time dynamics of SAP97/hDLG
title Nanoscale regulation of Ca(2+) dependent phase transitions and real-time dynamics of SAP97/hDLG
title_full Nanoscale regulation of Ca(2+) dependent phase transitions and real-time dynamics of SAP97/hDLG
title_fullStr Nanoscale regulation of Ca(2+) dependent phase transitions and real-time dynamics of SAP97/hDLG
title_full_unstemmed Nanoscale regulation of Ca(2+) dependent phase transitions and real-time dynamics of SAP97/hDLG
title_short Nanoscale regulation of Ca(2+) dependent phase transitions and real-time dynamics of SAP97/hDLG
title_sort nanoscale regulation of ca(2+) dependent phase transitions and real-time dynamics of sap97/hdlg
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307800/
https://www.ncbi.nlm.nih.gov/pubmed/35869063
http://dx.doi.org/10.1038/s41467-022-31912-1
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