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Flagellar Motility During E. coli Biofilm Formation Provides a Competitive Disadvantage Which Recedes in the Presence of Co-Colonizers

In nature, bacteria form biofilms in very diverse environments, involving a range of specific properties and exhibiting competitive advantages for surface colonization. However, the underlying mechanisms are difficult to decipher. In particular, the contribution of cell flagellar motility to biofilm...

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Autores principales: Benyoussef, Wafa, Deforet, Maxime, Monmeyran, Amaury, Henry, Nelly
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307998/
https://www.ncbi.nlm.nih.gov/pubmed/35880077
http://dx.doi.org/10.3389/fcimb.2022.896898
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author Benyoussef, Wafa
Deforet, Maxime
Monmeyran, Amaury
Henry, Nelly
author_facet Benyoussef, Wafa
Deforet, Maxime
Monmeyran, Amaury
Henry, Nelly
author_sort Benyoussef, Wafa
collection PubMed
description In nature, bacteria form biofilms in very diverse environments, involving a range of specific properties and exhibiting competitive advantages for surface colonization. However, the underlying mechanisms are difficult to decipher. In particular, the contribution of cell flagellar motility to biofilm formation remains unclear. Here, we examined the ability of motile and nonmotile E. coli cells to form a biofilm in a well-controlled geometry, both in a simple situation involving a single-species biofilm and in the presence of co-colonizers. Using a millifluidic channel, we determined that motile cells have a clear disadvantage in forming a biofilm, exhibiting a long delay as compared to nonmotile cells. By monitoring biofilm development in real time, we observed that the decisive impact of flagellar motility on biofilm formation consists in the alteration of surface access time potentially highly dependent on the geometry of the environment to be colonized. We also report that the difference between motile and nonmotile cells in the ability to form a biofilm diminishes in the presence of co-colonizers, which could be due to motility inhibition through the consumption of key resources by the co-colonizers. We conclude that the impact of flagellar motility on surface colonization closely depends on the environment properties and the population features, suggesting a unifying vision of the role of cell motility in surface colonization and biofilm formation.
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spelling pubmed-93079982022-07-24 Flagellar Motility During E. coli Biofilm Formation Provides a Competitive Disadvantage Which Recedes in the Presence of Co-Colonizers Benyoussef, Wafa Deforet, Maxime Monmeyran, Amaury Henry, Nelly Front Cell Infect Microbiol Cellular and Infection Microbiology In nature, bacteria form biofilms in very diverse environments, involving a range of specific properties and exhibiting competitive advantages for surface colonization. However, the underlying mechanisms are difficult to decipher. In particular, the contribution of cell flagellar motility to biofilm formation remains unclear. Here, we examined the ability of motile and nonmotile E. coli cells to form a biofilm in a well-controlled geometry, both in a simple situation involving a single-species biofilm and in the presence of co-colonizers. Using a millifluidic channel, we determined that motile cells have a clear disadvantage in forming a biofilm, exhibiting a long delay as compared to nonmotile cells. By monitoring biofilm development in real time, we observed that the decisive impact of flagellar motility on biofilm formation consists in the alteration of surface access time potentially highly dependent on the geometry of the environment to be colonized. We also report that the difference between motile and nonmotile cells in the ability to form a biofilm diminishes in the presence of co-colonizers, which could be due to motility inhibition through the consumption of key resources by the co-colonizers. We conclude that the impact of flagellar motility on surface colonization closely depends on the environment properties and the population features, suggesting a unifying vision of the role of cell motility in surface colonization and biofilm formation. Frontiers Media S.A. 2022-07-08 /pmc/articles/PMC9307998/ /pubmed/35880077 http://dx.doi.org/10.3389/fcimb.2022.896898 Text en Copyright © 2022 Benyoussef, Deforet, Monmeyran and Henry https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Benyoussef, Wafa
Deforet, Maxime
Monmeyran, Amaury
Henry, Nelly
Flagellar Motility During E. coli Biofilm Formation Provides a Competitive Disadvantage Which Recedes in the Presence of Co-Colonizers
title Flagellar Motility During E. coli Biofilm Formation Provides a Competitive Disadvantage Which Recedes in the Presence of Co-Colonizers
title_full Flagellar Motility During E. coli Biofilm Formation Provides a Competitive Disadvantage Which Recedes in the Presence of Co-Colonizers
title_fullStr Flagellar Motility During E. coli Biofilm Formation Provides a Competitive Disadvantage Which Recedes in the Presence of Co-Colonizers
title_full_unstemmed Flagellar Motility During E. coli Biofilm Formation Provides a Competitive Disadvantage Which Recedes in the Presence of Co-Colonizers
title_short Flagellar Motility During E. coli Biofilm Formation Provides a Competitive Disadvantage Which Recedes in the Presence of Co-Colonizers
title_sort flagellar motility during e. coli biofilm formation provides a competitive disadvantage which recedes in the presence of co-colonizers
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307998/
https://www.ncbi.nlm.nih.gov/pubmed/35880077
http://dx.doi.org/10.3389/fcimb.2022.896898
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