Endogenous spacing enables co-processing of microRNAs and efficient combinatorial RNAi

We present Multi-miR, a microRNA-embedded shRNA system modeled after endogenous microRNA clusters that enables simultaneous expression of up to three or four short hairpin RNAs (shRNAs) from a single promoter without loss of activity, enabling robust combinatorial RNA interference (RNAi). We further...

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Detalles Bibliográficos
Autores principales: Amen, Alexandra M., Loughran, Ryan M., Huang, Chun-Hao, Lew, Rachel J., Ravi, Archna, Guan, Yuanzhe, Schatoff, Emma M., Dow, Lukas E., Emerling, Brooke M., Fellmann, Christof
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9308131/
https://www.ncbi.nlm.nih.gov/pubmed/35880017
http://dx.doi.org/10.1016/j.crmeth.2022.100239

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9308131/
https://www.ncbi.nlm.nih.gov/pubmed/35880017
http://dx.doi.org/10.1016/j.crmeth.2022.100239

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