Cargando…

Endogenous spacing enables co-processing of microRNAs and efficient combinatorial RNAi

We present Multi-miR, a microRNA-embedded shRNA system modeled after endogenous microRNA clusters that enables simultaneous expression of up to three or four short hairpin RNAs (shRNAs) from a single promoter without loss of activity, enabling robust combinatorial RNA interference (RNAi). We further...

Descripción completa

Detalles Bibliográficos
Autores principales:	Amen, Alexandra M., Loughran, Ryan M., Huang, Chun-Hao, Lew, Rachel J., Ravi, Archna, Guan, Yuanzhe, Schatoff, Emma M., Dow, Lukas E., Emerling, Brooke M., Fellmann, Christof
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9308131/ https://www.ncbi.nlm.nih.gov/pubmed/35880017 http://dx.doi.org/10.1016/j.crmeth.2022.100239

Ejemplares similares

Crucial Players for Inter-Organelle Communication: PI5P4Ks and Their Lipid Product PI-4,5-P(2) Come to the Surface
por: Ravi, Archna, et al.
Publicado: (2022)

Targeting phosphoinositide signaling in cancer: relevant techniques to study lipids and novel avenues for therapeutic intervention
por: Llorente, Alicia, et al.
Publicado: (2023)

Combinatorial Pooling Enables Selective Sequencing of the Barley Gene Space
por: Lonardi, Stefano, et al.
Publicado: (2013)

A comparison of multiple shRNA expression methods for combinatorial RNAi
por: Mcintyre, Glen J, et al.
Publicado: (2011)

Extracting quantitative genetic interaction phenotypes from matrix combinatorial RNAi
por: Axelsson, Elin, et al.
Publicado: (2011)

Combinatorial RNAi: A Winning Strategy for the Race Against Evolving Targets?
por: Grimm, Dirk, et al.
Publicado: (2007)

Endogenous RNAi and adaptation to environment in C. elegans
por: Grishok, Alla
Publicado: (2012)

Combinatorial RNAi Against HIV-1 Using Extended Short Hairpin RNAs
por: Liu, Ying Poi, et al.
Publicado: (2009)

Combinatorial delivery of small interfering RNAs reduces RNAi efficacy by selective incorporation into RISC
por: Castanotto, Daniela, et al.
Publicado: (2007)

AAV-mediated in vivo knockdown of luciferase using combinatorial RNAi and U1i
por: Koornneef, A, et al.
Publicado: (2011)

Transplantation of engineered organoids enables rapid generation of metastatic mouse models of colorectal cancer
por: O’Rourke, Kevin P, et al.
Publicado: (2017)

Maternal and zygotic gene regulatory effects of endogenous RNAi pathways
por: Almeida, Miguel Vasconcelos, et al.
Publicado: (2019)

Dynamic control of endogenous metabolism with combinatorial logic circuits
por: Moser, Felix, et al.
Publicado: (2018)

R-Spondin chromosome rearrangements drive Wnt-dependent tumour initiation and maintenance in the intestine
por: Han, Teng, et al.
Publicado: (2017)

PSMA brings new flavors to PI3K signaling: A role for glutamate in prostate cancer
por: Palamiuc, Lavinia, et al.
Publicado: (2018)

Regulatory logic of endogenous RNAi in silencing de novo genomic conflicts
por: Vedanayagam, Jeffrey, et al.
Publicado: (2023)

Host-directed combinatorial RNAi improves inhibition of diverse strains of influenza A virus in human respiratory epithelial cells
por: Estrin, Michael A., et al.
Publicado: (2018)

Evaluation of a combinatorial RNAi lentivirus vector targeting foot-and-mouth disease virus in vitro and in vivo
por: ZHANG, XIAOXI, et al.
Publicado: (2015)

Ex vivo and in vivo suppression of SARS-CoV-2 with combinatorial AAV/RNAi expression vectors
por: Becker, Jonas, et al.
Publicado: (2022)

Large scale statistical inference of signaling pathways from RNAi and microarray data
por: Froehlich, Holger, et al.
Publicado: (2007)

ORP5 regulates PI(4)P on the lipid droplet: Novel players on the monolayer
por: Renne, Mike F., et al.
Publicado: (2019)

Optimized base editors enable efficient editing in cells, organoids and mice
por: Zafra, Maria Paz, et al.
Publicado: (2018)

Endogenous RNAi pathway evolutionarily shapes the destiny of the antisense lncRNAs transcriptome
por: Szachnowski, Ugo, et al.
Publicado: (2019)

Combinatorial assembly platform enabling engineering of genetically stable metabolic pathways in cyanobacteria
por: Taylor, George M, et al.
Publicado: (2021)

A Conserved PHD Finger Protein and Endogenous RNAi Modulate Insulin Signaling in Caenorhabditis elegans
por: Mansisidor, Andres R., et al.
Publicado: (2011)

Moment maps and combinatorial invariants of Hamiltonian Tn-spaces
por: Guillemin, Victor
Publicado: (1994)

Combinatorial Neural Inhibition for Stimulus Selection across Space
por: Mahajan, Nagaraj R., et al.
Publicado: (2018)

Spaces of Phylogenetic Diversity Indices: Combinatorial and Geometric Properties
por: Manson, Kerry, et al.
Publicado: (2023)

Guanidinium-Functionalized Interpolyelectrolyte Complexes Enabling RNAi in Resistant Insect Pests
por: Parsons, Keith H., et al.
Publicado: (2018)

Combinatorial Antimicrobial Susceptibility Testing Enabled by Non-Contact Printing
por: Opalski, Adam S., et al.
Publicado: (2020)

RNAi Development
por: Gerstein, Mark, et al.
Publicado: (2007)

Developmental programming modulates olfactory behavior in C. elegans via endogenous RNAi pathways
por: Sims, Jennie R, et al.
Publicado: (2016)

A Combinatorial Cell and Drug Delivery Strategy for Huntington’s Disease Using Pharmacologically Active Microcarriers and RNAi Neuronally-Committed Mesenchymal Stromal Cells
por: André, Emilie M., et al.
Publicado: (2019)

RNAi2013: RNAi at Oxford
por: Mulcahy, Laura A, et al.
Publicado: (2013)

In silico optimization of a guava antimicrobial peptide enables combinatorial exploration for peptide design
por: Porto, William F., et al.
Publicado: (2018)

A novel platform to enable inhaled naked RNAi medicine for lung cancer
por: Fujita, Yu, et al.
Publicado: (2013)

PI5P4Kα supports prostate cancer metabolism and exposes a survival vulnerability during androgen receptor inhibition
por: Triscott, Joanna, et al.
Publicado: (2023)

Combinatorial synthesis enables scalable designer detergents for membrane protein studies
por: Urner, Leonhard H., et al.
Publicado: (2022)

Combinatorial protein dimerization enables precise multi-input synthetic computations
por: Bertschi, Adrian, et al.
Publicado: (2023)

The surveillance of pre-mRNA splicing is an early step in C. elegans RNAi of endogenous genes
por: Newman, Martin A., et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_i73stsfrijfnqo6iuk67uce0mp