Cargando…

Boosting corrects a memory B cell defect in SARS-CoV-2 mRNA–vaccinated patients with inflammatory bowel disease

Immunosuppressed patients with inflammatory bowel disease (IBD) generate lower amounts of SARS-CoV-2 spike antibodies after mRNA vaccination than healthy controls. We assessed SARS-CoV-2 spike S1 receptor binding domain–specific (S1-RBD–specific) B lymphocytes to identify the underlying cellular def...

Descripción completa

Detalles Bibliográficos
Autores principales: Pape, Kathryn A., Dileepan, Thamotharampillai, Matchett, William E., Ellwood, Charles, Stresemann, Samuel, Kabage, Amanda J., Kozysa, Daria, Evert, Clayton, Matson, Michael, Lopez, Sharon, Krueger, Peter D., Graiziger, Carolyn T., Vaughn, Byron P., Shmidt, Eugenia, Rhein, Joshua, Schacker, Timothy W., Bold, Tyler D., Langlois, Ryan A., Khoruts, Alexander, Jenkins, Marc K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Clinical Investigation 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309052/
https://www.ncbi.nlm.nih.gov/pubmed/35730567
http://dx.doi.org/10.1172/jci.insight.159618
_version_ 1784753072856104960
author Pape, Kathryn A.
Dileepan, Thamotharampillai
Matchett, William E.
Ellwood, Charles
Stresemann, Samuel
Kabage, Amanda J.
Kozysa, Daria
Evert, Clayton
Matson, Michael
Lopez, Sharon
Krueger, Peter D.
Graiziger, Carolyn T.
Vaughn, Byron P.
Shmidt, Eugenia
Rhein, Joshua
Schacker, Timothy W.
Bold, Tyler D.
Langlois, Ryan A.
Khoruts, Alexander
Jenkins, Marc K.
author_facet Pape, Kathryn A.
Dileepan, Thamotharampillai
Matchett, William E.
Ellwood, Charles
Stresemann, Samuel
Kabage, Amanda J.
Kozysa, Daria
Evert, Clayton
Matson, Michael
Lopez, Sharon
Krueger, Peter D.
Graiziger, Carolyn T.
Vaughn, Byron P.
Shmidt, Eugenia
Rhein, Joshua
Schacker, Timothy W.
Bold, Tyler D.
Langlois, Ryan A.
Khoruts, Alexander
Jenkins, Marc K.
author_sort Pape, Kathryn A.
collection PubMed
description Immunosuppressed patients with inflammatory bowel disease (IBD) generate lower amounts of SARS-CoV-2 spike antibodies after mRNA vaccination than healthy controls. We assessed SARS-CoV-2 spike S1 receptor binding domain–specific (S1-RBD–specific) B lymphocytes to identify the underlying cellular defects. Patients with IBD produced fewer anti–S1-RBD antibody–secreting B cells than controls after the first mRNA vaccination and lower amounts of total and neutralizing antibodies after the second. S1-RBD–specific memory B cells were generated to the same degree in IBD and control groups and were numerically stable for 5 months. However, the memory B cells in patients with IBD had a lower S1-RBD–binding capacity than those in controls, which is indicative of a defect in antibody affinity maturation. Administration of a third shot to patients with IBD elevated serum antibodies and generated memory B cells with a normal antigen-binding capacity. These results show that patients with IBD have defects in the formation of antibody-secreting B cells and affinity-matured memory B cells that are corrected by a third vaccination.
format Online
Article
Text
id pubmed-9309052
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher American Society for Clinical Investigation
record_format MEDLINE/PubMed
spelling pubmed-93090522022-07-27 Boosting corrects a memory B cell defect in SARS-CoV-2 mRNA–vaccinated patients with inflammatory bowel disease Pape, Kathryn A. Dileepan, Thamotharampillai Matchett, William E. Ellwood, Charles Stresemann, Samuel Kabage, Amanda J. Kozysa, Daria Evert, Clayton Matson, Michael Lopez, Sharon Krueger, Peter D. Graiziger, Carolyn T. Vaughn, Byron P. Shmidt, Eugenia Rhein, Joshua Schacker, Timothy W. Bold, Tyler D. Langlois, Ryan A. Khoruts, Alexander Jenkins, Marc K. JCI Insight Research Article Immunosuppressed patients with inflammatory bowel disease (IBD) generate lower amounts of SARS-CoV-2 spike antibodies after mRNA vaccination than healthy controls. We assessed SARS-CoV-2 spike S1 receptor binding domain–specific (S1-RBD–specific) B lymphocytes to identify the underlying cellular defects. Patients with IBD produced fewer anti–S1-RBD antibody–secreting B cells than controls after the first mRNA vaccination and lower amounts of total and neutralizing antibodies after the second. S1-RBD–specific memory B cells were generated to the same degree in IBD and control groups and were numerically stable for 5 months. However, the memory B cells in patients with IBD had a lower S1-RBD–binding capacity than those in controls, which is indicative of a defect in antibody affinity maturation. Administration of a third shot to patients with IBD elevated serum antibodies and generated memory B cells with a normal antigen-binding capacity. These results show that patients with IBD have defects in the formation of antibody-secreting B cells and affinity-matured memory B cells that are corrected by a third vaccination. American Society for Clinical Investigation 2022-06-22 /pmc/articles/PMC9309052/ /pubmed/35730567 http://dx.doi.org/10.1172/jci.insight.159618 Text en © 2022 Pape et al. https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Pape, Kathryn A.
Dileepan, Thamotharampillai
Matchett, William E.
Ellwood, Charles
Stresemann, Samuel
Kabage, Amanda J.
Kozysa, Daria
Evert, Clayton
Matson, Michael
Lopez, Sharon
Krueger, Peter D.
Graiziger, Carolyn T.
Vaughn, Byron P.
Shmidt, Eugenia
Rhein, Joshua
Schacker, Timothy W.
Bold, Tyler D.
Langlois, Ryan A.
Khoruts, Alexander
Jenkins, Marc K.
Boosting corrects a memory B cell defect in SARS-CoV-2 mRNA–vaccinated patients with inflammatory bowel disease
title Boosting corrects a memory B cell defect in SARS-CoV-2 mRNA–vaccinated patients with inflammatory bowel disease
title_full Boosting corrects a memory B cell defect in SARS-CoV-2 mRNA–vaccinated patients with inflammatory bowel disease
title_fullStr Boosting corrects a memory B cell defect in SARS-CoV-2 mRNA–vaccinated patients with inflammatory bowel disease
title_full_unstemmed Boosting corrects a memory B cell defect in SARS-CoV-2 mRNA–vaccinated patients with inflammatory bowel disease
title_short Boosting corrects a memory B cell defect in SARS-CoV-2 mRNA–vaccinated patients with inflammatory bowel disease
title_sort boosting corrects a memory b cell defect in sars-cov-2 mrna–vaccinated patients with inflammatory bowel disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309052/
https://www.ncbi.nlm.nih.gov/pubmed/35730567
http://dx.doi.org/10.1172/jci.insight.159618
work_keys_str_mv AT papekathryna boostingcorrectsamemorybcelldefectinsarscov2mrnavaccinatedpatientswithinflammatoryboweldisease
AT dileepanthamotharampillai boostingcorrectsamemorybcelldefectinsarscov2mrnavaccinatedpatientswithinflammatoryboweldisease
AT matchettwilliame boostingcorrectsamemorybcelldefectinsarscov2mrnavaccinatedpatientswithinflammatoryboweldisease
AT ellwoodcharles boostingcorrectsamemorybcelldefectinsarscov2mrnavaccinatedpatientswithinflammatoryboweldisease
AT stresemannsamuel boostingcorrectsamemorybcelldefectinsarscov2mrnavaccinatedpatientswithinflammatoryboweldisease
AT kabageamandaj boostingcorrectsamemorybcelldefectinsarscov2mrnavaccinatedpatientswithinflammatoryboweldisease
AT kozysadaria boostingcorrectsamemorybcelldefectinsarscov2mrnavaccinatedpatientswithinflammatoryboweldisease
AT evertclayton boostingcorrectsamemorybcelldefectinsarscov2mrnavaccinatedpatientswithinflammatoryboweldisease
AT matsonmichael boostingcorrectsamemorybcelldefectinsarscov2mrnavaccinatedpatientswithinflammatoryboweldisease
AT lopezsharon boostingcorrectsamemorybcelldefectinsarscov2mrnavaccinatedpatientswithinflammatoryboweldisease
AT kruegerpeterd boostingcorrectsamemorybcelldefectinsarscov2mrnavaccinatedpatientswithinflammatoryboweldisease
AT graizigercarolynt boostingcorrectsamemorybcelldefectinsarscov2mrnavaccinatedpatientswithinflammatoryboweldisease
AT vaughnbyronp boostingcorrectsamemorybcelldefectinsarscov2mrnavaccinatedpatientswithinflammatoryboweldisease
AT shmidteugenia boostingcorrectsamemorybcelldefectinsarscov2mrnavaccinatedpatientswithinflammatoryboweldisease
AT rheinjoshua boostingcorrectsamemorybcelldefectinsarscov2mrnavaccinatedpatientswithinflammatoryboweldisease
AT schackertimothyw boostingcorrectsamemorybcelldefectinsarscov2mrnavaccinatedpatientswithinflammatoryboweldisease
AT boldtylerd boostingcorrectsamemorybcelldefectinsarscov2mrnavaccinatedpatientswithinflammatoryboweldisease
AT langloisryana boostingcorrectsamemorybcelldefectinsarscov2mrnavaccinatedpatientswithinflammatoryboweldisease
AT khorutsalexander boostingcorrectsamemorybcelldefectinsarscov2mrnavaccinatedpatientswithinflammatoryboweldisease
AT jenkinsmarck boostingcorrectsamemorybcelldefectinsarscov2mrnavaccinatedpatientswithinflammatoryboweldisease